Immunophenotyping by Flow Cytometry CD14 Test
Short Name: CD14 Flow Cytometry
Also known as: CD14 Immunophenotyping, Monocyte Immunophenotyping by Flow Cytometry
Immunophenotyping by Flow Cytometry CD14 Test test available at DNA Labs India for ₹3,500. Uses Flow Cytometry on Whole blood, Bone marrow samples. Results in Sample received by 9 am; report available same day.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
To identify and quantify CD14-positive monocytes and macrophages for diagnosis and monitoring of blood cancers and immune disorders.
- Test Code
- 3490
- Price
- ₹3,500
- Sample Type
- Whole blood, Bone marrow
- Result Time
- Sample received by 9 am; report available same day.
- Fasting Required
- No
- Method
- Flow Cytometry
Sample Collection
Give brief clinical history. No fasting required. Specify time, date and clinical details on test request form.
Method: Venipuncture or bone marrow aspiration
Laboratory Analysis
Blood is collected from a vein in the arm using standard aseptic technique. Bone marrow aspiration is performed by a trained clinician if required.
Report Delivery
Apply pressure at puncture site to prevent hematoma. Sample must be shipped immediately at 18-22°C; do not refrigerate or freeze.
Timeline: Sample received by 9 am; report available same day.
Patient Instructions
About This Test
Who Should Get This Test
To identify and quantify CD14-positive monocytes and macrophages for diagnosis and monitoring of blood cancers and immune disorders.
How to Prepare
- Collect 3 mL whole blood in Lavender Top (EDTA) tube
- Collect 3 mL whole blood in Green Top (Sodium Heparin) tube
- Alternatively, collect 2 mL bone marrow in Green Top (Sodium Heparin) tube
- Mix gently by inversion; do not shake vigorously
- Ship immediately at 18-22°C. Do not refrigerate or freeze
- Specify time, date, and clinical details on the test request form
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"CD14 immunophenotyping is a valuable tool for identifying monocytic lineage involvement in hematological malignancies and for monitoring immune status in inflammatory conditions. Results should always be correlated with clinical and morphological findings."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Clotted specimen
- Hemolyzed sample
- Insufficient volume
- Frozen or refrigerated sample
- Improperly labelled or missing clinical information
Understanding Your Results
May indicate infection, inflammation, autoimmune disease, or monocytic leukemia. Requires clinical correlation.
May be seen in bone marrow suppression, aplastic anemia, or post-chemotherapy states.
Altered antigen density can be seen in myelodysplastic syndromes or leukemic transformation.
Consult your healthcare provider if you experience unexplained fever, weight loss, night sweats, persistent fatigue, recurrent infections, easy bruising, or swollen lymph nodes.
Limitations
- ⚠Flow cytometry results should be interpreted in conjunction with clinical findings, morphology, and other laboratory tests
- ⚠CD14 is not specific for malignancy; abnormal levels may occur in benign reactive conditions
- ⚠Reference ranges are laboratory-dependent and may vary based on gating strategy and instrument settings
- ⚠Bone marrow samples require specialized collection and may have limited stability
Risks & Considerations
- ●Mild pain or bruising at venipuncture site
- ●Hematoma formation
- ●Rarely, infection or excessive bleeding
- ●Bone marrow aspiration may cause transient discomfort
Interfering Factors
- ●Recent infections or inflammatory conditions may transiently alter monocyte counts
- ●Corticosteroid or immunosuppressive therapy can affect CD14 expression
- ●Chemotherapy or growth factors may influence leukocyte populations
- ●Improper sample handling, refrigeration, or freezing can lead to cell loss and inaccurate results
- ●Clotted or hemolyzed specimens may be unsuitable for analysis
Compare With Similar Tests
| Test | Immunophenotyping by Flow Cytometry CD14 Test | |||
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| Comparison | Immunophenotyping by Flow Cytometry CD14 Test |
Frequently Asked Questions
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