Immunophenotyping by Flow Cytometry CD20 Test
Short Name: CD20 Flow Cytometry
Also known as: CD20 Immunophenotyping, Flow Cytometry CD20, B-Cell CD20 Marker Test
Immunophenotyping by Flow Cytometry CD20 Test test available at DNA Labs India for ₹3,500. Uses Flow Cytometry on Whole blood or bone marrow samples. Results in Sample daily by 9 am; report same day.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
To identify and characterize CD20-positive B-cell populations in blood or bone marrow, aiding the diagnosis, classification, and monitoring of B-cell leukemias and lymphomas.
- Test Code
- 3493
- Price
- ₹3,500
- Sample Type
- Whole blood or bone marrow
- Result Time
- Sample daily by 9 am; report same day.
- Fasting Required
- No
- Method
- Flow Cytometry
Sample Collection
Provide brief clinical history, including any prior cancer treatment, especially anti-CD20 therapy. No fasting is required.
Method: Venipuncture for whole blood; bone marrow aspiration by a clinician
Laboratory Analysis
For whole blood, a standard venipuncture is performed. For bone marrow, a trained specialist collects the sample under aseptic conditions.
Report Delivery
Samples must be shipped immediately at 18–22°C. No specific post-collection restrictions for the patient.
Timeline: Sample daily by 9 am; report same day.
Patient Instructions
About This Test
Who Should Get This Test
To identify and characterize CD20-positive B-cell populations in blood or bone marrow, aiding the diagnosis, classification, and monitoring of B-cell leukemias and lymphomas.
How to Prepare
- Collect 3 mL whole blood in Lavender Top (EDTA) tube and 3 mL whole blood in Green Top (Sodium Heparin) tube, OR 2 mL bone marrow in Green Top (Sodium Heparin) tube.
- Gently invert tubes to mix; do not shake.
- Label with time, date and clinical details.
- Ship immediately at 18–22°C; do not refrigerate or freeze.
- Provide brief clinical history on test request form.
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"CD20 flow cytometry is essential for classifying mature B-cell neoplasms and guiding anti-CD20 therapy. Always interpret in combination with morphology, clinical findings, and other immunophenotypic markers."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Clotted sample
- Hemolyzed sample
- Refrigerated or frozen sample
- Insufficient volume
- Sample delayed beyond 24 hours at room temperature
Understanding Your Results
CD20-positive mature B-cell population
Confirms B-cell lineage; may support B-cell lymphoma/leukemia when clonal
Clonal B-cell population with CD20 expression
Supports diagnosis of B-cell non-Hodgkin lymphoma or leukemia
Reduced or absent CD20
May occur after anti-CD20 therapy or in certain plasma cell/plasmablastic neoplasms
Consult a hematologist or oncologist if you have unexplained fever, night sweats, weight loss, lymph node swelling, fatigue, or abnormal blood counts.
Limitations
- ⚠Flow cytometry requires viable, adequately transported cells
- ⚠Low cell counts may limit analysis
- ⚠Results should be interpreted with clinical and morphological findings
Risks & Considerations
- ●Mild pain or bruising at venipuncture site
- ●Bone marrow aspiration may cause transient pain or bleeding
Interfering Factors
- ●Prior anti-CD20 therapy (e.g., rituximab) may reduce or mask CD20 expression
- ●Hemolyzed or clotted samples
- ●Delayed transport beyond recommended stability
- ●Incorrect sample collection or storage (refrigeration/freezing)
Frequently Asked Questions
What is Immunophenotyping by Flow Cytometry CD20 test?
Why is the CD20 test performed?
What sample is required for the test?
Do I need to fast before the CD20 test?
How is the test performed?
What is CD20?
What cancers can this test help detect?
How much does the Immunophenotyping by Flow Cytometry CD20 Test cost?
How long will it take to get results?
Is home sample collection available?
What do positive CD20 results mean?
What symptoms may require this test?
Related Tests
Reference Laboratory Services
We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.
Your Data Privacy
Your medical data is protected under Indian law.
✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.
✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.
Book Your Test
Enter your details and we'll connect you within 15 minutes.