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DNA Labs India

Immunophenotyping by Flow Cytometry CD22 Test

DNA Labs India | ISO 9001:2015 Certified

Immunophenotyping by Flow Cytometry CD22 Test

Short Name: CD22 Immunophenotyping

Also known as: CD22 Immunophenotyping, Flow Cytometry CD22 Test, CD22 Flow Cytometry Panel

Immunophenotyping by Flow Cytometry CD22 Test test available at DNA Labs India for ₹3,500. Uses Flow Cytometry on Whole blood or bone marrow samples. Results in Sample received daily by 9 am; report is delivered the same day.. Free home collection in 300+ cities across India.

Oncologist, HematologistAll age groups🏠 Home Collection

🩺 Medically Reviewed By

Overview

The CD22 test identifies the type of cells present in blood or bone marrow to diagnose various blood cancers and hematological disorders. It is part of immunophenotyping by flow cytometry and helps classify B-cell malignancies.

Test Code
3492
Price
₹3,500
Sample Type
Whole blood or bone marrow
Result Time
Sample received daily by 9 am; report is delivered the same day.
Fasting Required
No
Method
Flow Cytometry
Step 1

Sample Collection

Give brief clinical history on the test request form. No fasting is required.

Method: Venipuncture or bone marrow aspiration

Step 2

Laboratory Analysis

Blood sample is collected by venipuncture. Bone marrow sample is collected by a trained clinician using aseptic technique.

Step 3

Report Delivery

Specimen must be kept at 18–22°C and shipped immediately. Do not refrigerate or freeze.

Timeline: Sample received daily by 9 am; report is delivered the same day.

Patient Instructions

1
Before the Test:Provide brief clinical history. No fasting is required.
2
During the Test:A blood or bone marrow sample is collected by a trained professional.
3
After the Test:Keep sample at 18–22°C and transport immediately. Do not refrigerate or freeze.

About This Test

Who Should Get This Test

The CD22 test identifies the type of cells present in blood or bone marrow to diagnose various blood cancers and hematological disorders. It is part of immunophenotyping by flow cytometry and helps classify B-cell malignancies.

How to Prepare

  • Collect 3 mL whole blood in Lavender Top (EDTA) and 3 mL whole blood in Green Top (Sodium Heparin); or 2 mL bone marrow in Green Top (Sodium Heparin).
  • Maintain sample at 18–22°C during transport.
  • Do not refrigerate or freeze the specimen.
  • Specify time, date, and clinical details on the test request form.

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"CD22 is a pan B-cell marker. Its expression should always be interpreted as part of a complete flow cytometry panel for accurate diagnosis of B-cell malignancies."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeWhole blood or bone marrow
Sample Volume3 mL whole blood in each of two tubes OR 2 mL bone marrow
ContainerLavender Top (EDTA) and Green Top (Sodium Heparin); bone marrow in Green Top (Sodium Heparin)
Collection MethodVenipuncture or bone marrow aspiration

Sample Stability

Room temperature: 24 hours
Refrigerated: Not applicable
Frozen: Not applicable
Sample Rejection Criteria:
  • Hemolysed or clotted sample
  • Improper temperature during transport
  • Insufficient sample volume
  • Unlabeled or mismatched sample

Understanding Your Results

CD22 is normally expressed on mature B lymphocytes. Aberrant, absent, or altered CD22 expression may be seen in B-cell malignancies and must be interpreted with the complete immunophenotypic panel.
CD22 positive B-cell population with typical markers suggests normal or reactive B cells.
Loss or aberrant CD22 expression may support diagnosis of B-cell leukemia or lymphoma.
Results should be integrated with morphology, cytogenetics, and clinical findings by the treating oncologist or hematologist.
⚠️ When to Consult a Doctor:

Consult an oncologist or hematologist if CD22 flow cytometry shows an abnormal cell population or if symptoms such as persistent fever, unexplained weight loss, night sweats, bone pain, or enlarged lymph nodes are present.

Limitations

  • CD22 alone is insufficient for diagnosis; a full flow cytometry panel is required
  • Interpretation requires an experienced pathologist
  • Sampling error may cause false-negative results

Risks & Considerations

  • Mild pain or bruising at venipuncture site
  • Bone marrow aspiration may cause temporary discomfort

Interfering Factors

  • Hemolysed or clotted sample
  • Inadequate sample volume
  • Delay in transport or improper temperature
  • Recent chemotherapy, immunotherapy, or G-CSF therapy
  • Hemodilution of bone marrow

Compare With Similar Tests

TestImmunophenotyping by Flow Cytometry CD22 Test
ComparisonImmunophenotyping by Flow Cytometry CD22 Test

Frequently Asked Questions

What is Immunophenotyping by Flow Cytometry CD22 Test?
It is a diagnostic test that uses flow cytometry to identify CD22 expression on cells in blood or bone marrow. It helps classify B-cell populations and diagnose blood cancers or hematological disorders.
Why is CD22 testing done?
CD22 testing is done to identify the type of cells present in the blood or bone marrow and to help diagnose and classify blood cancers such as B-cell leukemias and lymphomas.
What are the symptoms of blood cancers that may require this test?
Common symptoms include fever, fatigue, unexplained weight loss, night sweats, bone pain, enlarged lymph nodes, and easy bruising or bleeding.
How is the test performed?
A small sample of blood or bone marrow is collected and then analyzed in the laboratory by flow cytometry to determine the type of cells present.
Is fasting required for the CD22 flow cytometry test?
No fasting is required. You only need to give a brief clinical history before sample collection.
What sample type is required?
3 mL whole blood in a Lavender Top (EDTA) tube and 3 mL whole blood in a Green Top (Sodium Heparin) tube, or 2 mL bone marrow in a Green Top (Sodium Heparin) tube.
What is the cost of Immunophenotyping by Flow Cytometry CD22 Test at DNA Labs India?
The cost is INR 3500, and free home sample collection is available for online bookings across India.
How long does it take to get the report?
If the sample is received by 9 am, the report is delivered the same day.
Can I get home sample collection?
Yes, DNA Labs India offers free home sample collection for online bookings for this test.
What conditions can this test help diagnose?
It can help diagnose various blood cancers and hematological disorders, especially B-cell leukemias and lymphomas.
Is the CD22 flow cytometry test painful?
A blood draw may cause mild pain or bruising. Bone marrow aspiration may cause temporary discomfort but is performed by trained clinicians.
How should the sample be transported?
The sample should be shipped immediately at 18–22°C. Do not refrigerate or freeze the specimen.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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