Immunophenotyping by Flow Cytometry CD34 (Stem Cell) Enumeration Test
Short Name: CD34 Enumeration Test
Also known as: CD34+ Cell Quantification, Stem Cell Enumeration Test, Flow Cytometry CD34 Test
Immunophenotyping by Flow Cytometry CD34 (Stem Cell) Enumeration Test test available at DNA Labs India for ₹3,500. Uses Flow Cytometry on Whole blood, Bone marrow, Cord blood, Apheresis sample samples. Results in Same day. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
CD34+ cell quantification is necessary to determine the optimal timing and to confirm the adequacy of Peripheral Blood Stem Cell (PBSC) harvests. This assay also helps to determine when to perform Bone marrow stem cell transplants; whether to continue Apheresis and to assess bone marrow and Cord blood viability. CD34+ absolute count is a critical parameter for rapid and sustained engraftment.
- Test Code
- 1075
- Price
- ₹3,500
- Sample Type
- Whole blood, Bone marrow, Cord blood, Apheresis sample
- Result Time
- Same day
- Fasting Required
- No
- Method
- Flow Cytometry
Sample Collection
Provide brief clinical history and inform the laboratory of any relevant medications or conditions.
Method: Venipuncture for blood; Bone marrow aspiration; Cord blood collection
Laboratory Analysis
Sample collection via venipuncture for blood or aspiration for bone marrow. The procedure is performed by a trained phlebotomist.
Report Delivery
Apply pressure to the puncture site to prevent bleeding. Resume normal activities.
Timeline: Same day
Patient Instructions
About This Test
Who Should Get This Test
CD34+ cell quantification is necessary to determine the optimal timing and to confirm the adequacy of Peripheral Blood Stem Cell (PBSC) harvests. This assay also helps to determine when to perform Bone marrow stem cell transplants; whether to continue Apheresis and to assess bone marrow and Cord blood viability. CD34+ absolute count is a critical parameter for rapid and sustained engraftment.
How to Prepare
- For whole blood: Collect 3 mL in Lavender Top (EDTA) tube and 3 mL in Green Top (Sodium Heparin) tube
- For bone marrow: Collect 2 mL in Green Top (Sodium Heparin) tube
- For cord blood or apheresis: Collect 1 mL in a sterile screw capped container
- Ship immediately at specified temperatures: 18-22°C for blood/bone marrow, 2-8°C for cord blood/apheresis
- Do not freeze samples
- Specify time, date, and clinical details on test request form
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"This test is essential for accurate stem cell enumeration in transplantation and cancer care, ensuring optimal treatment timing and patient outcomes."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed or clotted samples
- Samples not stored at recommended temperatures
- Insufficient sample volume
- Improperly labeled samples
Understanding Your Results
Elevated CD34+ count
May indicate active stem cell mobilization, certain leukemias, or recovery after chemotherapy.
Low CD34+ count
Suggests inadequate stem cell harvest or bone marrow failure; may affect transplant success.
Normal CD34+ count
Within expected range for the clinical context; further evaluation may be needed based on symptoms.
If you experience symptoms such as persistent fatigue, unexplained weight loss, frequent infections, or abnormal bleeding, consult a doctor. Abnormal test results should be discussed with a hematologist or oncologist for proper management.
Limitations
- ⚠Requires specialized flow cytometry equipment and trained personnel
- ⚠Results should be interpreted in conjunction with clinical findings and other tests
Risks & Considerations
- ●Minor bruising or pain at the puncture site
- ●Rare risk of infection or excessive bleeding
- ●Dizziness during blood draw
Interfering Factors
- ●Sample hemolysis or clotted blood
- ●Improper sample storage or delay in processing
- ●Use of certain medications that affect stem cell mobilization
Frequently Asked Questions
What is the Immunophenotyping by Flow Cytometry CD34 (Stem Cell) Enumeration Test?
Why is this test recommended?
What samples are required for the test?
What do elevated CD34+ levels indicate?
What do low CD34+ levels mean?
How soon are results available?
Is fasting required before the test?
Can this test alone diagnose cancer?
How reliable is the CD34 enumeration test?
Are there any risks associated with the test?
What is the cost of the test?
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