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Immunophenotyping by Flow Cytometry: Leukemia Diagnostic Panel-AML Characterization Test

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Immunophenotyping by Flow Cytometry: Leukemia Diagnostic Panel-AML Characterization Test

Short Name: AML Immunophenotyping Panel

Also known as: AML Immunophenotyping, AML Flow Cytometry Panel, Leukemia Diagnostic Panel-AML Characterization

Immunophenotyping by Flow Cytometry: Leukemia Diagnostic Panel-AML Characterization Test test available at DNA Labs India for ₹14,500. Uses Flow Cytometry on Whole blood or bone marrow samples. Results in Same day if sample is received by 9 am. Free home collection in 300+ cities across India.

Flow CytometryAll age groups🏠 Home Collection

🩺 Medically Reviewed By

Overview

The test aids in diagnosis, classification, therapeutic monitoring and detection of relapse in acute myeloid leukemia.

Test Code
3503
CPT Code
88182
ICD Code
C92.0
Price
₹14,500
Sample Type
Whole blood or bone marrow
Result Time
Same day if sample is received by 9 am
Fasting Required
No
Method
Flow Cytometry
Step 1

Sample Collection

No fasting is required. Clinical history is mandatory.

Method: Peripheral venous blood draw or bone marrow aspiration by trained personnel

Step 2

Laboratory Analysis

For blood collection, a tourniquet is applied and a venipuncture is performed. For bone marrow, a trained clinician performs aspiration from the posterior iliac crest under aseptic conditions.

Step 3

Report Delivery

Apply pressure at the collection site. For bone marrow aspiration, keep the site clean and dry as instructed by the clinician.

Timeline: Same day if sample is received by 9 am

Patient Instructions

1
Before the Test:No fasting is required. Provide complete clinical history and relevant blood counts if available.
2
During the Test:Blood or bone marrow sample is collected by a healthcare professional. Flow cytometry analysis is performed in the laboratory.
3
After the Test:No specific aftercare is needed for a blood draw. Following bone marrow aspiration, follow the clinician's site care instructions.

About This Test

Who Should Get This Test

The test aids in diagnosis, classification, therapeutic monitoring and detection of relapse in acute myeloid leukemia.

How to Prepare

  • Collect 3 mL whole blood in 1 Lavender Top EDTA tube and 1 Green Top Sodium Heparin tube
  • Alternatively collect 2 mL bone marrow in 1 Green Top Sodium heparin tube along with aspirate smear
  • Ship immediately at 18 to 22 degrees Celsius
  • Do not refrigerate or freeze
  • Clinical history is mandatory

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"Flow cytometric immunophenotyping is essential for AML diagnosis and classification. Accurate gating and mandatory clinical history are critical to avoid misinterpretation of lineage markers."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeWhole blood or bone marrow
Sample Volume3 mL whole blood (2 mL min.) in EDTA and sodium heparin tubes OR 2 mL bone marrow (1 mL min.) in sodium heparin tube
Container1 Lavender Top (EDTA) tube AND 1 Green Top (Sodium Heparin) tube OR 1 Green Top (Sodium heparin) tube for bone marrow plus aspirate smear
Collection MethodPeripheral venous blood draw or bone marrow aspiration by trained personnel

Sample Stability

Room temperature: 24 hours
Refrigerator: Not applicable
Frozen: Not applicable
Sample Rejection Criteria:
  • Clotted sample
  • Sample received refrigerated or frozen
  • Inadequate sample volume
  • Missing clinical history
  • Improper container or tube

Understanding Your Results

Interpretation is based on the expression of surface and cytoplasmic markers on blasts. A positive myeloid lineage marker profile supports AML. Correlation with clinical history, morphology and molecular genetics is required.
📊

Supports acute myeloid leukemia with myeloid lineage

📊

Suggests monocytic differentiation

📊

May indicate mixed phenotype acute leukemia or aberrant expression

📊

Consider lymphoid or undifferentiated leukemia; further markers may be needed

⚠️ When to Consult a Doctor:

Consult your doctor if you have symptoms such as unexplained fatigue, fever, night sweats, easy bruising, frequent infections, or if blood counts are abnormal.

Limitations

  • Flow cytometry results must be interpreted with morphology, cytogenetics and molecular studies
  • A negative or inconclusive panel does not exclude leukemia
  • Sample viability and adequate cell count are required for reliable gating
  • Aberrant marker expression may overlap between AML and mixed phenotype acute leukemia

Risks & Considerations

  • Minor bruising or pain at blood draw site
  • Transient discomfort during bone marrow aspiration
  • Small bleeding risk at aspiration site
  • Rare infection at aspiration site

Interfering Factors

  • Hemodilution during bone marrow collection
  • Delayed transportation
  • Clotted sample
  • Refrigerated or frozen sample
  • Recent chemotherapy or growth factor administration

Compare With Similar Tests

TestImmunophenotyping by Flow Cytometry: Leukemia Diagnostic Panel-AML Characterization Test
ComparisonImmunophenotyping by Flow Cytometry: Leukemia Diagnostic Panel-AML Characterization Test

Frequently Asked Questions

What is the Immunophenotyping by Flow Cytometry AML Characterization Test?
It is a flow cytometry-based diagnostic test that uses fluorescent antibodies to detect cell surface and intracellular markers on leukemic blasts. It helps diagnose and classify acute myeloid leukemia.
How much does the test cost?
The test is priced at Rs 14500.0 at DNA Labs India. Online booking includes free home sample collection in select cities.
What sample is required?
3 mL whole blood in EDTA and sodium heparin tubes, or 2 mL bone marrow in sodium heparin with an aspirate smear. Sample must be shipped at 18 to 22 degrees Celsius.
Is fasting required?
No fasting is required. However, a mandatory clinical history is required before testing.
How is the test performed?
A blood or bone marrow sample is stained with fluorochrome-tagged antibodies. A flow cytometer analyzes the cells, identifying lineage and maturation markers.
When will I get the report?
Reports are usually available the same day if the sample is received by 9 am. Reports are delivered online, by email, and WhatsApp.
What conditions does this test diagnose?
It is primarily used for diagnosis and characterization of acute myeloid leukemia, and can help differentiate AML from other acute leukemias.
Are there any risks?
For blood draw, minor bruising or pain may occur. Bone marrow aspiration may cause transient discomfort and small bleeding risk.
Can the test detect minimal residual disease?
This panel is designed for diagnosis and characterization. MRD testing may use a separate, patient-specific flow panel.
What is the difference between AML and ALL immunophenotyping?
AML panels focus on myeloid markers like MPO, CD13, CD33, CD117, CD14 and CD64; ALL panels focus on B- or T-lymphoid markers such as CD19, CD10, CD3 and CD7.
Is the test covered by insurance?
Insurance coverage varies by provider and policy. Some private insurers may cover it if medically indicated; please check with your provider.
How do I book the test?
You can book online at DNA Labs India for the discounted price of Rs 14500. Free home collection is available in many Indian cities.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

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Your medical data is protected under Indian law.

Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

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