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DNA Labs India

DNA Genetic Methylation Test

DNA Labs India | ISO 9001:2015 Certified

DNA Genetic Methylation Test

Short Name: MGMT Methylation Test

Also known as: MGMT Methylation Assay, DNA Methylation Test, MGMT Promoter Methylation Test

DNA Genetic Methylation Test test available at DNA Labs India for ₹15,000. Uses MLPA (Methylation-Specific Multiplex Ligation-Dependent Probe Amplification), Bisulfite Conversion, Confirmatory Analysis on Blood samples. Results in 15-20 business days from sample receipt. Free home collection in 300+ cities across India.

Molecular Genetic Test🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr Pasupathy Arumugam

Dr Pasupathy Arumugam

Consultant Pathologist · Reg: 21521

Last reviewed: September 7, 2026

Overview

To determine the methylation status of the MGMT promoter gene for prognosis in glioblastoma and to assess overall methylation pathway efficiency for metabolic health, disease risk evaluation, and personalized treatment planning.

Test Code
3088
Price
₹15,000
Sample Type
Blood
Result Time
15-20 business days from sample receipt
Fasting Required
No
Method
MLPA (Methylation-Specific Multiplex Ligation-Dependent Probe Amplification), Bisulfite Conversion, Confirmatory Analysis
Step 1

Sample Collection

No fasting required. Inform the phlebotomist about any medications, supplements, or recent treatments. Ensure adequate hydration.

Method: Venipuncture

Step 2

Laboratory Analysis

Blood sample will be collected via venipuncture from a vein in the arm. The phlebotomist will use a sterile needle and collect into an EDTA tube, maintaining cold chain protocol.

Step 3

Report Delivery

Apply pressure to the puncture site to prevent bruising. The sample will be transported in a 2-8°C specialized container to the lab. Avoid strenuous activity for a few hours.

Timeline: 15-20 business days from sample receipt

Patient Instructions

1
Before the Test:No special preparation needed. Provide complete clinical history and list of medications to the healthcare provider.
2
During the Test:A blood sample will be drawn from your arm. The procedure takes a few minutes and is minimally invasive.
3
After the Test:You can resume normal activities immediately. Wait for the report, which will be delivered online or via email/WhatsApp. Genetic counselling is available post-results.

About This Test

Who Should Get This Test

To determine the methylation status of the MGMT promoter gene for prognosis in glioblastoma and to assess overall methylation pathway efficiency for metabolic health, disease risk evaluation, and personalized treatment planning.

How to Prepare

  • Use EDTA tube for blood collection
  • Maintain sample at 2-8°C during transport
  • Ensure transport to lab within 6 hours of collection
  • Label sample correctly with patient details

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"Samples are transported in 2–8°C specialized containers to maintain fragile DNA methylation patterns. Our internal 2025-26 logistics data confirms that 98% of samples from Bangalore and Hyderabad reach our central processing unit within 6 hours, ensuring peak enzyme stability for fragile epigenetic markers."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeBlood
Sample Volume5 mL
ContainerEDTA tube with cold chain container
Collection MethodVenipuncture

Sample Stability

Stable at 2-8°C for up to 24 hours
For long-term storage, keep at -20°C or below
Avoid repeated freeze-thaw cycles
Sample Rejection Criteria:
  • Hemolyzed or clotted sample
  • Incorrect container or insufficient volume
  • Sample not stored at required temperature
  • Missing or mismatched patient information

Understanding Your Results

Results indicate the methylation status of the MGMT promoter and other genes in the panel. Methylated MGMT promoter suggests favorable prognosis for temozolomide therapy in glioblastoma, while unmethylated status may require alternative treatments. Gene variants provide insights into methylation pathway efficiency and associated health risks.
📊

MGMT gene silenced, better response to alkylating chemotherapy like temozolomide

Status: Methylated MGMT

📊

MGMT gene active, DNA repair may reduce chemotherapy efficacy; consider alternative targeted therapies

Status: Unmethylated MGMT

📊

Downregulation may elevate homocysteine levels, increasing cardiovascular and neural tube defect risks

📊

Downregulation impairs neurotransmitter breakdown, potentially affecting mood and estrogen metabolism

⚠️ When to Consult a Doctor:

Consult an oncologist if MGMT is unmethylated for alternative therapy options. Consult a genetic counsellor or physician for abnormal gene variants to discuss personalized health management, lifestyle modifications, and monitoring plans.

Limitations

  • ⚠Does not detect all possible methylation variants or epigenetic changes
  • ⚠Results should be interpreted in conjunction with clinical history and other diagnostics
  • ⚠Not a standalone diagnostic for cancer; used for prognosis and guidance
  • ⚠Limited to the genes in the panel; does not cover all methylation-related genes

Risks & Considerations

  • ●Minor bruising or pain at the puncture site
  • ●Rare risk of infection or fainting
  • ●No significant long-term risks associated with the test

Interfering Factors

  • ●Sample contamination
  • ●Improper storage temperature (deviation from 2-8°C)
  • ●Degraded DNA due to delayed transport
  • ●Hemolyzed or lipemic samples

Compare With Similar Tests

TestDNA Genetic Methylation Test
ComparisonDNA Genetic Methylation Test

Frequently Asked Questions

Is the MGMT Methylation test covered under home collection in my city?
Yes, DNA Labs India offers free home sample collection across 300+ Tier-1 and Tier-2 Indian cities, including Mumbai, Delhi, Bengaluru, Hyderabad, Chennai, Kolkata, Pune, Ahmedabad, Lucknow, and more. Our phlebotomists follow cold chain 2-8°C protocol. Book via call or WhatsApp at 09395142800.
How does the 2026 DNA Labs India price compare to hospital rates?
Hospital and third-party lab rates for MGMT methylation (MLPA) typically range from ?22,000 to ?35,000. DNA Labs India offers the test at ?15,000 all-inclusive, with ISO certification, same methodology, and free genetic counselling. No hidden charges apply.
What is the significance of 'methylated' vs 'unmethylated' results?
Methylated MGMT promoter means the gene is silenced, leading to better response to alkylating chemotherapies like temozolomide in glioblastoma. Unmethylated means the gene is active, DNA repair may reduce chemotherapy efficacy, and alternative targeted therapies may be considered. Our counselling team explains these nuances.
Why is the turnaround time 15–20 days for the methylation assay?
The MLPA (methylation-specific multiplex ligation-dependent probe amplification) plus bisulfite conversion requires strict quality controls, duplicate runs, and validation by senior molecular pathologists. We prioritize accuracy over speed to ensure reliable results.
What genes are included in the MGMT Methylation Test?
The test examines a curated panel including MGMT, MTHFR (C677T & A1298C), COMT V158M, CBS C699T, MTR, MTRR, BHMT, GNMT, MAT1A, and SHMT1. These genes are critical for methylation pathways, detoxification, DNA repair, and metabolic health.
How is the sample collected and transported?
A blood sample is collected via venipuncture into an EDTA tube. It is then placed in specialized 2-8°C cold chain containers and transported to our lab within 6 hours to maintain DNA integrity, as confirmed by our logistics data.
Is genetic counselling included with the test?
Yes, complimentary genetic counselling is included with every MGMT Methylation Test. Our certified experts help interpret complex markers like MTHFR, COMT, and MGMT promoter status in relation to your clinical history.
What should I do if my results are abnormal?
If results show unmethylated MGMT or abnormal gene variants, consult an oncologist for cancer treatment options or a genetic counsellor for personalized health management. Our team provides guidance on next steps.
Can this test be used for cancer screening?
No, the MGMT Methylation Test is not a standalone cancer screening tool. It is used for prognosis in glioblastoma and to assess methylation pathway efficiency. For cancer screening, consult a healthcare provider for appropriate tests.
How accurate is the MGMT Methylation Test?
The test uses ISO 15189 certified MLPA methodology with bisulfite conversion and confirmatory analysis, ensuring high accuracy. Internal validation shows 98% sample integrity from key cities, but results should be interpreted clinically.
Are there any risks associated with the test?
Risks are minimal and similar to a standard blood draw, including minor bruising, pain at the puncture site, or rare infection. No significant long-term risks are associated with the test.
How do I book the MGMT Methylation Test?
You can book the test by calling or WhatsApping 09395142800 (available 8 AM – 8 PM IST). Free home collection is available across India, and our team will assist with scheduling and queries.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

LIMS Integration

Your Data Privacy

Your medical data is protected under Indian law.

✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

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