Vitamin B1 (Thiamine Pyrophosphate) Blood Test
Short Name: Vitamin B1 TPP Blood Test
Also known as: Vitamin B1 Test, Thiamine Test, Thiamine Pyrophosphate Test, TPP Blood Test
Vitamin B1 (Thiamine Pyrophosphate) Blood Test test available at DNA Labs India for ₹11,000. Uses High-Performance Liquid Chromatography (HPLC), Fluorescence Detection on Whole Blood samples. Results in The test is run by HPLC and typically reported within 3 days after the sample reaches the laboratory. For samples collected by Friday 9 AM, reports are available by Tuesday.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Ramarao Paidisetty
Consultant Biochemist · Reg: 21504
Last reviewed: September 7, 2026
Overview
To evaluate the level of thiamine pyrophosphate (TPP) in whole blood to diagnose vitamin B1 deficiency, assess high-risk conditions, and monitor the effectiveness of thiamine supplementation.
- Test Code
- 3748
- Price
- ₹11,000
- Sample Type
- Whole Blood
- Result Time
- The test is run by HPLC and typically reported within 3 days after the sample reaches the laboratory. For samples collected by Friday 9 AM, reports are available by Tuesday.
- Fasting Required
- No
- Method
- High-Performance Liquid Chromatography (HPLC), Fluorescence Detection
Sample Collection
No special preparation is needed. No fasting is required. Please inform your doctor about any supplements, vitamins, or medications you are taking.
Method: Venipuncture
Laboratory Analysis
A trained phlebotomist will draw a small volume of blood from a vein in your arm. The procedure is quick and generally painless.
Report Delivery
You can resume normal activities immediately. A small bruise may appear at the puncture site but subsides within a few days.
Timeline: The test is run by HPLC and typically reported within 3 days after the sample reaches the laboratory. For samples collected by Friday 9 AM, reports are available by Tuesday.
Patient Instructions
About This Test
Who Should Get This Test
To evaluate the level of thiamine pyrophosphate (TPP) in whole blood to diagnose vitamin B1 deficiency, assess high-risk conditions, and monitor the effectiveness of thiamine supplementation.
How to Prepare
- Collect blood in a lavender top (EDTA) tube.
- Fill the tube completely to the required volume (at least 2 mL).
- Immediately wrap the tube tightly with black paper or aluminium foil to protect from light.
- Transport the sample refrigerated or frozen to the laboratory.
Doctor's Notes
Reviewed by Dr Ramarao Paidisetty — MBBS, MD (Biochemistry) · Reg. No. 21504
"Vitamin B1 deficiency can present with non-specific symptoms. A TPP assay is a gold-standard test to assess total body thiamine status. However, clinical correlation is essential because low levels may not always correlate with symptoms."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Uncovered tubes exposed to light are unacceptable.
- Hemolyzed samples are rejected.
- Clotted samples are not suitable for analysis.
Understanding Your Results
Normal
Recommendation: No deficiency. Maintain a balanced diet with adequate thiamine intake.
Range: 70 - 180 nmol/L
Deficient
Recommendation: Vitamin B1 deficiency is likely. Consult a physician; consider thiamine supplementation.
Range: < 70 nmol/L
Elevated
Recommendation: Usually benign; may occur with recent thiamine supplementation.
Range: > 180 nmol/L
If you have symptoms suggestive of vitamin B1 deficiency such as persistent fatigue, weakness, memory loss, confusion, or numbness and tingling in your hands or feet, see a doctor. Patients with chronic alcohol use, malabsorption, or those undergoing long-term dialysis should also consult for routine screening.
Limitations
- ⚠Test measures TPP in whole blood, not free thiamine; results may not always correlate perfectly with clinical symptoms.
- ⚠The reference range may vary slightly between laboratories.
- ⚠Patients who took thiamine supplements before testing may show false-normal levels.
Risks & Considerations
- ●Slight pain or discomfort during blood draw
- ●Bruising or swelling at the puncture site
- ●Rarely, infection or excessive bleeding
Interfering Factors
- ●Exposure of blood sample to light can degrade thiamine compounds.
- ●Hemolysis may affect test accuracy.
- ●Prolonged storage or improper transport conditions can alter results.
Compare With Similar Tests
| Test | Vitamin B1 (Thiamine Pyrophosphate) Blood Test | Vitamin B12 Test | Folate Test | Homocysteine Test |
|---|---|---|---|---|
| Comparison | Vitamin B1 (Thiamine Pyrophosphate) Blood Test |
Frequently Asked Questions
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