Vitamin B2 Riboflavin Test
Short Name: Riboflavin Test
Also known as: Vitamin B2 Test, Riboflavin Blood Test, Riboflavin Assay
Vitamin B2 Riboflavin Test test available at DNA Labs India for ₹13,000. Uses LC-MS/MS on Plasma from Lavender Top (EDTA) tube samples. Results in Samples received by the 7th of the month; reports are issued after 2–3 weeks.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Ramarao Paidisetty
Consultant Biochemist · Reg: 21504
Last reviewed: September 7, 2026
Overview
To measure plasma riboflavin concentration and assist in diagnosing vitamin B2 deficiency in patients with mucocutaneous lesions, anaemia, eye changes, or other symptoms suggestive of nutritional deficiency.
- Test Code
- 3737
- Price
- ₹13,000
- Sample Type
- Plasma from Lavender Top (EDTA) tube
- Result Time
- Samples received by the 7th of the month; reports are issued after 2–3 weeks.
- Fasting Required
- Yes
- Method
- LC-MS/MS
Sample Collection
Overnight fasting is mandatory. Avoid vitamin supplements for 24 hours before sample collection. Complete and sign the Test Send Out Consent Form (Form 35). Inform your doctor about any medicines or supplements you are taking.
Method: Venipuncture
Laboratory Analysis
A trained phlebotomist will collect approximately 2 mL of blood from a vein in your arm into a lavender top EDTA tube. The sample must be protected from light and processed according to the given instructions.
Report Delivery
You may resume normal activities immediately. There are no specific post-test restrictions unless advised by your doctor.
Timeline: Samples received by the 7th of the month; reports are issued after 2–3 weeks.
Patient Instructions
About This Test
Who Should Get This Test
To measure plasma riboflavin concentration and assist in diagnosing vitamin B2 deficiency in patients with mucocutaneous lesions, anaemia, eye changes, or other symptoms suggestive of nutritional deficiency.
How to Prepare
- Overnight fasting is mandatory.
- Use a lavender top (EDTA) tube.
- Separate plasma from cells within 3 hours of collection.
- Freeze plasma immediately and ship frozen.
- Do not thaw the sample.
- Wrap the sample in aluminium foil to protect from light.
- Avoid vitamin supplements 24 hours prior to sample collection.
- Duly fill the Test Send Out Consent Form (Form 35).
Doctor's Notes
Reviewed by Dr Ramarao Paidisetty — MBBS, MD (Biochemistry) · Reg. No. 21504
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Plasma not separated within 3 hours of collection
- Sample exposed to light
- Sample received at room temperature or refrigerated
- Sample received thawed
- Incomplete or missing Test Send Out Consent Form
Understanding Your Results
If you have persistent mouth sores, sore throat, redness or itching of the eyes, skin rash, fatigue, or if you have malabsorption or malnutrition risk factors, consult a physician before testing.
Limitations
- ⚠Plasma riboflavin may not fully reflect tissue stores in all cases
- ⚠Functional biomarkers such as EGRAC may be needed to assess vitamin B2 status
- ⚠Results must be interpreted with clinical history and other laboratory findings
- ⚠Recent supplementation can elevate riboflavin and mask deficiency
Risks & Considerations
- ●Minimal pain or bruising at the needle site
- ●Slight bleeding
- ●Rare infection
- ●Dizziness or fainting
Interfering Factors
- ●Recent intake of vitamin B2 supplements within 24 hours
- ●Exposure of sample to light
- ●Delayed separation of plasma from cells
- ●Haemolysis
- ●Severe illness or catabolic stress
Frequently Asked Questions
What is Vitamin B2 Riboflavin Test?
How much does the Vitamin B2 Riboflavin Test cost in India?
Do I need to fast before the test?
What sample is needed for the test?
Why is the sample wrapped in aluminium foil?
Can I take vitamin supplements before the test?
Is a consent form required?
How long will my reports take?
Is home sample collection available?
What do low riboflavin levels mean?
Is this test suitable for diagnosing all nutritional deficiencies?
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