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DNA Labs India

Vitamin B2 Riboflavin Test

DNA Labs India | ISO 9001:2015 Certified

Vitamin B2 Riboflavin Test

Short Name: Riboflavin Test

Also known as: Vitamin B2 Test, Riboflavin Blood Test, Riboflavin Assay

Vitamin B2 Riboflavin Test test available at DNA Labs India for ₹13,000. Uses LC-MS/MS on Plasma from Lavender Top (EDTA) tube samples. Results in Samples received by the 7th of the month; reports are issued after 2–3 weeks.. Free home collection in 300+ cities across India.

Nutritional Disorder TestAll Ages🏠 Home Collection

🩺 Medically Reviewed By

Overview

To measure plasma riboflavin concentration and assist in diagnosing vitamin B2 deficiency in patients with mucocutaneous lesions, anaemia, eye changes, or other symptoms suggestive of nutritional deficiency.

Test Code
3737
Price
₹13,000
Sample Type
Plasma from Lavender Top (EDTA) tube
Result Time
Samples received by the 7th of the month; reports are issued after 2–3 weeks.
Fasting Required
Yes
Method
LC-MS/MS
Step 1

Sample Collection

Overnight fasting is mandatory. Avoid vitamin supplements for 24 hours before sample collection. Complete and sign the Test Send Out Consent Form (Form 35). Inform your doctor about any medicines or supplements you are taking.

Method: Venipuncture

Step 2

Laboratory Analysis

A trained phlebotomist will collect approximately 2 mL of blood from a vein in your arm into a lavender top EDTA tube. The sample must be protected from light and processed according to the given instructions.

Step 3

Report Delivery

You may resume normal activities immediately. There are no specific post-test restrictions unless advised by your doctor.

Timeline: Samples received by the 7th of the month; reports are issued after 2–3 weeks.

Patient Instructions

1
Before the Test:Overnight fasting is mandatory. Avoid vitamin supplements 24 hours before sample collection. Complete the required consent form.
2
During the Test:A blood sample is drawn from a vein. The sample must be protected from light and frozen after processing.
3
After the Test:No specific after-care is needed. You can resume normal activities and diet unless advised otherwise by your doctor.

About This Test

Who Should Get This Test

To measure plasma riboflavin concentration and assist in diagnosing vitamin B2 deficiency in patients with mucocutaneous lesions, anaemia, eye changes, or other symptoms suggestive of nutritional deficiency.

How to Prepare

  • Overnight fasting is mandatory.
  • Use a lavender top (EDTA) tube.
  • Separate plasma from cells within 3 hours of collection.
  • Freeze plasma immediately and ship frozen.
  • Do not thaw the sample.
  • Wrap the sample in aluminium foil to protect from light.
  • Avoid vitamin supplements 24 hours prior to sample collection.
  • Duly fill the Test Send Out Consent Form (Form 35).

Doctor's Notes

Reviewed by — MBBS, MD (Biochemistry) · Reg. No. 21504

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypePlasma from Lavender Top (EDTA) tube
Sample Volume2 mL (1 mL min.) plasma
ContainerLavender Top (EDTA) tube
Collection MethodVenipuncture

Sample Stability

Room Temperature
Refrigerator (2-8°C)
Frozen
Sample Rejection Criteria:
  • Plasma not separated within 3 hours of collection
  • Sample exposed to light
  • Sample received at room temperature or refrigerated
  • Sample received thawed
  • Incomplete or missing Test Send Out Consent Form

Understanding Your Results

The plasma riboflavin level should be interpreted by a qualified doctor along with the patient's symptoms, diet history, and other tests.
Low riboflavin concentration with consistent clinical findings may indicate vitamin B2 deficiency.
Normal riboflavin concentration does not completely exclude functional deficiency; the physician may order additional functional tests such as EGRAC.
Recent supplementation can elevate riboflavin levels and mask deficiency.
Test result alone is not diagnostic; clinical correlation and nutritional assessment are required.
⚠️ When to Consult a Doctor:

If you have persistent mouth sores, sore throat, redness or itching of the eyes, skin rash, fatigue, or if you have malabsorption or malnutrition risk factors, consult a physician before testing.

Limitations

  • Plasma riboflavin may not fully reflect tissue stores in all cases
  • Functional biomarkers such as EGRAC may be needed to assess vitamin B2 status
  • Results must be interpreted with clinical history and other laboratory findings
  • Recent supplementation can elevate riboflavin and mask deficiency

Risks & Considerations

  • Minimal pain or bruising at the needle site
  • Slight bleeding
  • Rare infection
  • Dizziness or fainting

Interfering Factors

  • Recent intake of vitamin B2 supplements within 24 hours
  • Exposure of sample to light
  • Delayed separation of plasma from cells
  • Haemolysis
  • Severe illness or catabolic stress

Frequently Asked Questions

What is Vitamin B2 Riboflavin Test?
It is a blood test that measures the level of riboflavin (vitamin B2) in plasma. It is used to help identify riboflavin deficiency in people with suggestive symptoms or malabsorption risk.
How much does the Vitamin B2 Riboflavin Test cost in India?
At DNA Labs India, the test costs Rs 13000. The price includes free home sample collection for online bookings across many cities in India.
Do I need to fast before the test?
Yes. Overnight fasting is mandatory before sample collection.
What sample is needed for the test?
A blood sample is collected from a vein into a lavender top (EDTA) tube. At least 1 mL plasma is required; the laboratory generally receives 2 mL.
Why is the sample wrapped in aluminium foil?
Riboflavin is sensitive to light. The sample must be protected from light to prevent degradation and ensure accurate results.
Can I take vitamin supplements before the test?
No. Avoid all vitamin supplements for 24 hours before sample collection, as they can raise riboflavin levels and mask a deficiency.
Is a consent form required?
Yes. A duly filled Test Send Out Consent Form (Form 35) is mandatory for this test.
How long will my reports take?
The sample must reach the reference laboratory by the 7th of the month. Reports are available after 2–3 weeks.
Is home sample collection available?
Yes. DNA Labs India offers free home sample collection for online bookings of this test in major cities across India.
What do low riboflavin levels mean?
Low plasma riboflavin can indicate vitamin B2 deficiency, but the result must be interpreted by a doctor along with clinical signs and other investigations.
Is this test suitable for diagnosing all nutritional deficiencies?
No. This test specifically measures vitamin B2. Other vitamins such as B1, B6, B12, and folate require separate tests.
Can I see a doctor before getting the test?
Yes. It is advisable to consult a physician if you have symptoms such as mouth sores, sore throat, eye irritation, skin changes, or persistent fatigue. The doctor will decide if this test is appropriate for you.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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