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DNA Labs India

Vitamin B1 Thiamine Test

DNA Labs India | ISO 9001:2015 Certified

Vitamin B1 Thiamine Test

Short Name: Vitamin B1 Thiamine

Also known as: Thiamine Test, Vitamin B1 Test, Thiamine Blood Test

Vitamin B1 Thiamine Test test available at DNA Labs India for ₹7,605. Uses LC-MS/MS on Plasma / Serum samples. Results in Samples are processed in a batch. The test is performed on samples received by the 7th of the month; reports are issued after 2-3 weeks.. Free home collection in 300+ cities across India.

Blood TestAll Age Groups🏠 Home Collection

🩺 Medically Reviewed By

Overview

This test is used to detect vitamin B1 (thiamine) deficiency, especially in individuals presenting with symptoms of fatigue, neuropathy, or cognitive impairment, and in those at high risk due to chronic alcoholism, malabsorption, or prolonged inadequate diet.

Test Code
3749
Price
₹7,605
Sample Type
Plasma / Serum
Result Time
Samples are processed in a batch. The test is performed on samples received by the 7th of the month; reports are issued after 2-3 weeks.
Fasting Required
Yes (8 hours)
Method
LC-MS/MS
Step 1

Sample Collection

Fasting for at least 8 hours is required; overnight fasting is strongly recommended. Inform your physician about any medications you are taking, as certain drugs may influence thiamine levels.

Method: Venipuncture

Step 2

Laboratory Analysis

A blood sample will be obtained from a vein using a sterile needle. The process usually takes less than 5 minutes.

Step 3

Report Delivery

No special precautions are required. You may resume normal activities and dietary intake unless otherwise instructed.

Timeline: Samples are processed in a batch. The test is performed on samples received by the 7th of the month; reports are issued after 2-3 weeks.

Patient Instructions

1
Before the Test:Overnight fasting is mandatory. Avoid alcohol for 24 hours prior to the test. Inform your doctor about all medications, as some may affect thiamine levels.
2
During the Test:A blood sample is drawn from a vein in your arm. It takes only a few minutes and may cause minor discomfort.
3
After the Test:You can resume normal activities immediately. No special aftercare is required.

About This Test

Who Should Get This Test

This test is used to detect vitamin B1 (thiamine) deficiency, especially in individuals presenting with symptoms of fatigue, neuropathy, or cognitive impairment, and in those at high risk due to chronic alcoholism, malabsorption, or prolonged inadequate diet.

How to Prepare

  • Overnight fasting is mandatory.
  • Wrap sample in aluminium foil to protect from light.
  • Separate plasma/serum within 4 hours of collection and freeze.
  • Ship frozen. DO NOT THAW.
  • Duly filled Test Send Out Consent Form (Form 35) is mandatory.

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"Early detection of Vitamin B1 deficiency is vital, especially for individuals with chronic alcoholism, persistent vomiting, or malabsorptive conditions. The symptoms are often subtle and can be easily overlooked, leading to irreversible neurological damage if untreated."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypePlasma / Serum
Sample Volume3 mL (2 mL min.) plasma from 1 Lavender Top (EDTA) tube OR 2 mL (1 mL min.) serum from 1 SST
ContainerLavender Top (EDTA) tube OR SST (Serum Separator Tube)
Collection MethodVenipuncture

Sample Stability

Room TemperatureNot Accepted
RefrigeratorNot Stable
Frozen30 days
Sample Rejection Criteria:
  • Unfasted patient sample.
  • Sample exposed to light during transport.
  • Plasma/serum not separated within 4 hours of collection.
  • Sample received thawed.
  • Missing Test Send Out Consent Form (Form 35).
  • Clotted sample.

Understanding Your Results

Circulating thiamine levels are directly related to dietary intake and metabolic utilization. Low levels confirm a state of vitamin B1 deficiency.
📊

Low thiamine level

Indicates vitamin B1 deficiency and may be associated with symptoms like fatigue, neuropathy, confusion, and beriberi if severe.

📊

Normal thiamine level

Generally excludes a significant deficiency state.

📊

Elevated thiamine level

Rarely of clinical concern; excess water-soluble vitamins are usually excreted in urine.

⚠️ When to Consult a Doctor:

If you have symptoms suggestive of vitamin B1 deficiency, or if you are at high risk (e.g., chronic alcoholism, persistent vomiting, malabsorption), consult your physician promptly for interpretation of this test and appropriate management.

Limitations

  • The test reflects circulating thiamine at the time of collection and may not fully indicate tissue or total body stores.
  • Functional assays such as erythrocyte transketolase activity may be used in conjunction for a more comprehensive assessment.
  • This test is not intended for the evaluation of other vitamin deficiencies.

Risks & Considerations

  • Minimal risk of bleeding, bruising, or infection at the puncture site.

Interfering Factors

  • Failure to protect the sample from light may cause photodegradation of thiamine, leading to falsely low results.
  • Delayed separation of plasma/serum (beyond 4 hours) can alter thiamine stability.
  • Sample thawing during transport may reduce measured thiamine levels.
  • Haemolyzed or lipemic samples may interfere with the LC-MS/MS assay.

Frequently Asked Questions

What is the Vitamin B1 Thiamine Test?
The Vitamin B1 Thiamine Test is a laboratory blood test that measures the level of thiamine (Vitamin B1) in your blood. It is used to detect a deficiency and help in the clinical diagnosis of conditions like beriberi or Wernicke-Korsakoff syndrome.
Why has my doctor recommended this test?
Your doctor may recommend this test if you have symptoms such as fatigue, weakness, numbness or tingling in the extremities, confusion, memory problems, unexplained weight loss, or chronic alcoholism. It may also be ordered for individuals with malabsorption disorders or those on certain medications.
What are the common symptoms of Vitamin B1 deficiency?
Symptoms include fatigue, weakness, muscle pain, poor memory and concentration, loss of appetite, weight loss, irritability, mood swings, difficulty sleeping, and peripheral neuropathy (numbness/tingling in hands/feet).
What is the cost of the Vitamin B1 Thiamine Test at DNA Labs India?
The test is available at a discounted price of Rs 7605, inclusive of free home sample collection in select cities across India. The standard cost may vary depending on location and laboratory.
Is home sample collection available for this test?
Yes, DNA Labs India offers free home sample collection for online bookings. This service is available in more than 50 cities across India, including Mumbai, Delhi, Bangalore, Hyderabad, Chennai, Kolkata, Pune, and many more.
What is the preparation required for the test?
Overnight fasting is mandatory. You should also ensure that the sample is protected from light during collection and transport. A duly filled Test Send Out Consent Form (Form 35) is necessary.
What type of sample is collected?
A blood sample is drawn from a vein. A minimum of 2 mL of plasma (EDTA) or 1 mL of serum (SST) is required. The sample must be separated within 4 hours of collection and frozen.
What is the normal range for Vitamin B1?
The reference range is typically 2.5–7.5 µg/dL in serum/plasma, but it may vary between laboratories. Your report will include the specific range used for interpretation.
How long will it take to receive my report?
Samples must be received by the 7th of the month. The report is delivered after 2–3 weeks from the date of sample receipt.
Are there any risks associated with the blood test?
The test is a routine blood draw and carries minimal risk, including slight pain, bruising, or infection at the puncture site.
What does a low thiamine level indicate?
A low thiamine level confirms vitamin B1 deficiency. If left untreated, it can lead to serious neurological and cardiovascular complications such as beriberi and Wernicke-Korsakoff syndrome.
Can I take any medications before the test?
You should inform your doctor about all medications and supplements you are taking. Some drugs may interfere with thiamine levels. However, do not stop any medication unless directed by your physician.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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