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ANCA (Antineutrophil Cytoplasmic Antibodies) IFA in Dilutions Test

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ANCA (Antineutrophil Cytoplasmic Antibodies) IFA in Dilutions Test

Short Name: ANCA IFA Dilutions Test

Also known as: Anti-Neutrophil Cytoplasmic Antibody Test, ANCA Antibody Test, ANCA IFA Test

ANCA (Antineutrophil Cytoplasmic Antibodies) IFA in Dilutions Test test available at DNA Labs India for ₹5,000. Uses Immunofluorescence Assay on Serum samples. Results in Next day. Free home collection in 300+ cities across India.

Blood Test🏠 Home Collection

✍️ Written By

Overview

To diagnose ANCA-associated vasculitis and other autoimmune disorders by detecting ANCA antibodies in the blood.

Test Code
117
Price
₹5,000
Sample Type
Serum
Result Time
Next day
Fasting Required
Yes (8 hours)
Method
Immunofluorescence Assay
Step 1

Sample Collection

Overnight fasting is preferred. Avoid medications that may interfere unless advised by a doctor.

Method: Venipuncture

Step 2

Laboratory Analysis

A blood sample is drawn from a vein in the arm using standard venipuncture technique.

Step 3

Report Delivery

Apply pressure to the puncture site to prevent bruising. The sample is processed and stored at recommended temperatures.

Timeline: Next day

Patient Instructions

1
Before the Test:Fast overnight for 8-12 hours. Inform your doctor about any medications or supplements.
2
During the Test:A small blood sample is taken from your arm. The process is quick and minimally invasive.
3
After the Test:You can resume normal activities. Results are typically available the next day.

About This Test

Who Should Get This Test

To diagnose ANCA-associated vasculitis and other autoimmune disorders by detecting ANCA antibodies in the blood.

How to Prepare

  • Collect 2 mL (1 mL min.) serum from 1 SST
  • Ship refrigerated or frozen
  • Label the sample with patient details

Doctor's Notes

"ANCA testing is crucial for diagnosing ANCA-associated vasculitis, which requires timely treatment to prevent organ damage, especially in the kidneys and lungs."

Test Parameters & Specifications

Sample TypeSerum
Sample Volume2 mL (1 mL min.)
ContainerSST (Serum Separator Tube)
Collection MethodVenipuncture

Sample Stability

Room Temperature: 6 hours
Refrigerator: 1 week
Frozen: 2 weeks
Sample Rejection Criteria:
  • Hemolyzed or clotted samples
  • Insufficient volume
  • Improper labeling or storage

Understanding Your Results

Results indicate the presence and pattern of ANCA antibodies. Positive results suggest ANCA-associated vasculitis, but correlation with clinical symptoms is essential.
📊

Negative

No detectable ANCA antibodies. Does not rule out all vasculitis cases.

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Positive c-ANCA

Suggestive of granulomatosis with polyangiitis (GPA).

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Positive p-ANCA

Suggestive of microscopic polyangiitis (MPA) or eosinophilic granulomatosis with polyangiitis (EGPA).

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Positive with high titer

May indicate active disease or severity; monitor and correlate clinically.

⚠️ When to Consult a Doctor:

If you experience symptoms like fever, weight loss, joint pain, shortness of breath, or blood in urine, or if your test results are positive or ambiguous.

Limitations

  • ⚠May not distinguish between specific vasculitis subtypes
  • ⚠False positives can occur in infections or other autoimmune diseases
  • ⚠Results should be interpreted alongside clinical symptoms and other tests

Risks & Considerations

  • ●Minor bruising or pain at the needle site
  • ●Rare cases of dizziness or fainting
  • ●Very low risk of infection

Interfering Factors

  • ●Hemolyzed or lipemic samples
  • ●Recent infections or vaccinations
  • ●Certain medications like antibiotics or immunosuppressants

Frequently Asked Questions

What is the ANCA IFA in Dilutions test?
It is a blood test that uses indirect immunofluorescence to detect ANCA antibodies, associated with autoimmune vasculitis.
Why is this test performed?
To diagnose ANCA-associated vasculitis and related autoimmune diseases, helping to guide treatment.
What are the symptoms of ANCA-associated vasculitis?
Symptoms include fever, fatigue, weight loss, joint pain, shortness of breath, and skin rashes.
How should I prepare for the test?
Overnight fasting is preferred. Inform your doctor about any medications you are taking.
What is the cost of the ANCA IFA in Dilutions test?
The test costs Rs 5000.0 in India, with free home collection available in many cities.
What do the results mean?
Positive results indicate the presence of ANCA antibodies, suggesting vasculitis, but must be interpreted with clinical symptoms.
Are there any risks or side effects?
The test involves minimal risks like bruising at the needle site. Serious complications are very rare.
How long does it take to get the results?
Results are typically available the next day after sample collection.
Can this test diagnose all types of vasculitis?
It is specific for ANCA-associated vasculitis; other types may require different tests.
What is the difference between c-ANCA and p-ANCA?
c-ANCA is linked to granulomatosis with polyangiitis, while p-ANCA is associated with microscopic polyangiitis and EGPA.
Is the test covered by insurance?
Coverage depends on your insurance plan. Check with your provider for details.
How accurate is the ANCA IFA test?
It is highly sensitive and specific for detecting ANCA antibodies, but false positives/negatives can occur; clinical correlation is essential.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

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