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Anti-ds DNA Antibody Crithidia IFA in Dilutions Test

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Anti-ds DNA Antibody Crithidia IFA in Dilutions Test

Short Name: Anti-dsDNA Crithidia IFA Test

Also known as: Crithidia IFA Test, Anti-dsDNA Antibody Test

Anti-ds DNA Antibody Crithidia IFA in Dilutions Test test available at DNA Labs India for ₹4,500. Uses Immunofluorescence Assay on Serum samples. Results in Report available next day after sample collection.. Free home collection in 300+ cities across India.

ImmunologyAdults🏠 Home Collection

Overview

The primary purpose of this test is to detect and quantify anti-dsDNA antibodies in the blood, aiding in the diagnosis and management of autoimmune diseases such as lupus. It helps clinicians identify active disease flares, differentiate between autoimmune conditions, and guide therapeutic interventions to prevent complications like kidney damage.

Test Code
132
Price
₹4,500
Sample Type
Serum
Result Time
Report available next day after sample collection.
Fasting Required
Yes (8 hours)
Method
Immunofluorescence Assay
Step 1

Sample Collection

Overnight fasting is preferred for accurate results. Inform your doctor about any medications or supplements you are taking.

Method: Venipuncture

Step 2

Laboratory Analysis

A blood sample will be drawn from a vein in your arm using a needle. The procedure typically takes a few minutes and may cause minimal discomfort.

Step 3

Report Delivery

Apply pressure to the puncture site to prevent bleeding. You can resume normal activities immediately.

Timeline: Report available next day after sample collection.

Patient Instructions

1
Before the Test:Ensure overnight fasting and inform your doctor about any health conditions or medications.
2
During the Test:A blood draw is performed at a lab or via home collection. It is quick and minimally invasive.
3
After the Test:Results are typically available next day. Follow up with your doctor for interpretation.

About This Test

Who Should Get This Test

The primary purpose of this test is to detect and quantify anti-dsDNA antibodies in the blood, aiding in the diagnosis and management of autoimmune diseases such as lupus. It helps clinicians identify active disease flares, differentiate between autoimmune conditions, and guide therapeutic interventions to prevent complications like kidney damage.

How to Prepare

  • Fast for 8-12 hours before the test, preferably overnight
  • Sample: 3 mL serum from SST tube
  • Ship refrigerated or frozen to maintain stability

Doctor's Notes

"This test is essential for early detection and monitoring of autoimmune conditions like lupus, helping guide treatment decisions and prevent organ damage."

Test Parameters & Specifications

Sample TypeSerum
Sample Volume3 mL (1.5 mL min.)
ContainerSST (Serum Separator Tube)
Collection MethodVenipuncture

Sample Stability

Room Temperature: 2 hours
Refrigerator: 72 hours
Frozen: 2 weeks
Sample Rejection Criteria:
  • Hemolyzed or lipemic samples
  • Insufficient sample volume (< 1.5 mL)
  • Improperly labeled or contaminated samples

Understanding Your Results

Results are interpreted based on the titer of anti-dsDNA antibodies detected. A negative result suggests low likelihood of autoimmune activity, while a positive result indicates potential autoimmune disease, requiring further clinical correlation.
📊

Negative (< 1:10 dilution)

No significant anti-dsDNA antibodies detected; may indicate absence of active autoimmune disease.

Action: Consult physician if symptoms persist; consider other diagnostic tests.

📊

Positive (≥ 1:10 dilution)

Elevated anti-dsDNA antibodies, suggestive of autoimmune conditions like lupus. Higher titers correlate with increased disease activity.

Action: Immediate consultation with a rheumatologist or immunologist for further evaluation and management.

⚠️ When to Consult a Doctor:

If you experience symptoms such as persistent fatigue, joint pain, skin rashes, or if your test results are positive, consult a healthcare provider promptly for a comprehensive assessment and personalized treatment plan.

Limitations

  • Not specific to lupus; can be positive in other autoimmune diseases like mixed connective tissue disease
  • Results may vary based on disease stage and treatment
  • Does not replace comprehensive clinical evaluation and other diagnostic tests

Risks & Considerations

  • Minor bruising or pain at the blood draw site
  • Rare risk of infection or fainting
  • No significant long-term risks associated with the test

Interfering Factors

  • Medications such as immunosuppressants or biologics may affect results
  • Other autoimmune conditions can cause cross-reactivity
  • Recent infections or vaccinations may temporarily elevate antibody levels

Compare With Similar Tests

TestAnti-ds DNA Antibody Crithidia IFA in Dilutions TestANA (Antinuclear Antibody) TestAnti-Smith Antibody TestCRP (C-Reactive Protein) Test
ComparisonAnti-ds DNA Antibody Crithidia IFA in Dilutions Test

Frequently Asked Questions

What is the Anti-dsDNA Antibody Crithidia IFA in Dilutions Test?
It is a blood test that detects anti-double-stranded DNA antibodies using immunofluorescence with Crithidia luciliae, helping diagnose autoimmune diseases like lupus.
Why is this test done?
To diagnose or monitor autoimmune conditions, particularly systemic lupus erythematosus, by measuring specific antibody levels that indicate disease activity.
How should I prepare for the test?
Overnight fasting for 8-12 hours is preferred. Avoid medications that might interfere, and inform your doctor about any health issues.
What does a positive result mean?
A positive result suggests elevated anti-dsDNA antibodies, which are commonly associated with autoimmune diseases like lupus, indicating possible active disease.
What does a negative result mean?
A negative result means anti-dsDNA antibodies were not detected at significant levels, but it does not entirely rule out autoimmune disease if symptoms persist.
Is the test painful?
The test involves a standard blood draw, which may cause slight discomfort or bruising, but it is generally quick and well-tolerated.
How long does it take to get results?
Results are typically available the next day after sample collection, accessible online, via email, or WhatsApp.
Is home sample collection available?
Yes, DNA Labs India offers free home sample collection for this test across numerous cities in India for online bookings.
What are the risks of this test?
Risks are minimal, including minor bruising at the blood draw site, rare infection, or fainting. No significant long-term risks.
Can this test be done for children?
This test is typically for adults. For children, consult a pediatric rheumatologist for appropriate testing.
How accurate is the Crithidia IFA test?
It is highly specific for anti-dsDNA antibodies and is a standard method for lupus diagnosis, but results should be interpreted alongside clinical findings.
What should I do if my results are abnormal?
Consult a rheumatologist or immunologist immediately for further evaluation, additional tests, and to discuss treatment options.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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