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DNA Labs India

Anti Nuclear Antibody / Factor (ANA / ANF) EIA Test

DNA Labs India | ISO 9001:2015 Certified

Anti Nuclear Antibody / Factor (ANA / ANF) EIA Test

Short Name: ANA/ANF EIA Test

Also known as: ANA Test, ANF Test, Antinuclear Antibody Test

Anti Nuclear Antibody / Factor (ANA / ANF) EIA Test test available at DNA Labs India for ₹819. Uses Enzyme Immunoassay (EIA) on Serum samples. Results in Same day. Free home collection in 300+ cities across India.

Enzyme Immunoassay🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr SHAILAJA RAGHUNATH MURDESHWAR

Dr SHAILAJA RAGHUNATH MURDESHWAR

Consultant Physician · Reg: 8052

Last reviewed: September 7, 2026

Overview

To detect antinuclear antibodies in the blood, aiding in the diagnosis of autoimmune disorders such as lupus, rheumatoid arthritis, and other systemic conditions. It helps identify the presence of immune system abnormalities that may require further investigation or treatment.

Test Code
146
Price
₹819
Sample Type
Serum
Result Time
Same day
Fasting Required
Yes
Method
Enzyme Immunoassay (EIA)
Step 1

Sample Collection

Overnight fasting is preferred. Inform your doctor about any medications or supplements you are taking.

Method: Venipuncture

Step 2

Laboratory Analysis

A blood sample will be drawn from a vein in your arm using a needle. The procedure is quick and minimally invasive.

Step 3

Report Delivery

Apply pressure to the puncture site with a bandage to prevent bleeding. Resume normal activities unless advised otherwise.

Timeline: Same day

Patient Instructions

1
Before the Test:Overnight fasting is preferred. Avoid strenuous activity before the test.
2
During the Test:A small blood sample will be taken from your arm vein. The process takes only a few minutes.
3
After the Test:You may resume normal activities. Apply pressure to the site if needed.

About This Test

Who Should Get This Test

To detect antinuclear antibodies in the blood, aiding in the diagnosis of autoimmune disorders such as lupus, rheumatoid arthritis, and other systemic conditions. It helps identify the presence of immune system abnormalities that may require further investigation or treatment.

How to Prepare

  • Fast overnight (8-12 hours) if possible for accurate results.
  • Wear loose clothing to allow easy access to your arm.
  • Stay hydrated by drinking water before the test.
  • Inform the phlebotomist of any allergies or medical conditions.

Doctor's Notes

Reviewed by — MBBS, MD (General Medicine) · Reg. No. 8052

"This test is crucial for early detection of autoimmune disorders, allowing for timely management and improved patient outcomes."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeSerum
Sample Volume2 mL (1 mL min.)
ContainerSST (Serum Separator Tube)
Collection MethodVenipuncture

Sample Stability

Room Temperature8 hours
Refrigerator (2-8°C)48 hours
Frozen (-20°C)2 weeks
Sample Rejection Criteria:
  • Hemolyzed or lipemic samples
  • Insufficient sample volume
  • Incorrect sample container or labeling
  • Sample collected without proper fasting (if required)

Understanding Your Results

A positive ANA test indicates the presence of antinuclear antibodies, which may be associated with autoimmune disorders. However, a positive result alone is not diagnostic and must be correlated with clinical symptoms and additional tests.
📊

Positive ANA

Further testing recommended to identify specific autoimmune disorder (e.g., anti-dsDNA for lupus). Low titers may be seen in healthy individuals.

📊

Negative ANA

Autoimmune disorder is less likely, but does not completely rule it out if symptoms persist. Consider other causes.

📊

Borderline or Equivocal

Repeat testing or additional clinical evaluation may be needed to clarify results.

⚠️ When to Consult a Doctor:

If you experience symptoms such as persistent fatigue, joint pain, skin rashes, or unexplained fever, consult a healthcare provider to discuss the need for ANA testing and further evaluation.

Limitations

  • ⚠A positive ANA test does not confirm a specific autoimmune disorder; additional tests are needed for diagnosis.
  • ⚠False positives can occur in healthy individuals, especially with low titers.
  • ⚠The test does not differentiate between active disease and past exposure.
  • ⚠Results may vary based on laboratory methods and cut-off values.

Risks & Considerations

  • ●Minor bruising or swelling at the needle site
  • ●Rarely, infection or hematoma
  • ●Fainting or dizziness in some individuals

Interfering Factors

  • ●Certain medications (e.g., hydralazine, procainamide, isoniazid)
  • ●Recent viral or bacterial infections
  • ●Presence of other autoimmune or inflammatory conditions
  • ●Age-related factors, as ANA may be positive in elderly individuals without disease

Frequently Asked Questions

What is the ANA/ANF EIA test?
The ANA/ANF EIA test is a blood test that detects antinuclear antibodies, which are proteins produced by the immune system that may indicate autoimmune disorders such as lupus or rheumatoid arthritis.
How much does the ANA test cost at DNA Labs India?
The cost of the ANA/ANF EIA test at DNA Labs India is INR 819, which includes free home sample collection across India.
Is fasting required for the ANA test?
Overnight fasting is preferred for optimal results, but it is not mandatory. You may be advised to fast for 8-12 hours before the test.
How is the ANA test performed?
The test is performed using a blood sample collected from a vein in your arm. The sample is analyzed using Enzyme Immunoassay (EIA) to detect antinuclear antibodies.
What do ANA test results mean?
A positive ANA result indicates the presence of antinuclear antibodies, which may suggest an autoimmune disorder. However, a positive test does not confirm a specific disease and requires further evaluation.
How long does it take to get ANA test results?
At DNA Labs India, ANA test results are typically available on the same day, with reports delivered via online portal, email, or WhatsApp.
Is home collection available for the ANA test?
Yes, DNA Labs India offers free home sample collection for the ANA/ANF EIA test across numerous cities in India when booked online.
What symptoms indicate the need for an ANA test?
Symptoms such as persistent fatigue, joint pain or stiffness, skin rashes, swollen glands, unexplained weight loss, or chronic fever may warrant an ANA test.
Can a positive ANA test confirm lupus?
No, a positive ANA test alone cannot confirm lupus. Additional tests like anti-dsDNA antibody and clinical evaluation are needed for a definitive diagnosis.
What other tests might be needed after a positive ANA test?
Further tests may include Complete Blood Count (CBC), C-Reactive Protein (CRP), Erythrocyte Sedimentation Rate (ESR), and specific antibody tests like anti-dsDNA to identify the specific autoimmune disorder.
Are there any risks associated with the ANA test?
The ANA test is a simple blood draw with minimal risks, such as minor bruising, fainting, or rare infection at the puncture site.
How accurate is the ANA/ANF EIA test?
The ANA/ANF EIA test is highly accurate for detecting antinuclear antibodies, but accuracy depends on the laboratory method and clinical context. It is a reliable screening tool for autoimmune disorders.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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