Anti Nuclear Antibody / Factor (ANA / ANF) IFA (HEP-2) Reflex to ENA Profile Test
Short Name: ANA/ANF IFA Reflex to ENA
Also known as: ANA, ANF, Anti-nuclear Antibody, Anti-nuclear Factor
Anti Nuclear Antibody / Factor (ANA / ANF) IFA (HEP-2) Reflex to ENA Profile Test test available at DNA Labs India for ₹4,500. Uses Immunofluorescence Assay (Hep-2 Substrate), Immunoblot on Serum samples. Results in Reports are typically available the next working day after sample collection.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr SHAILAJA RAGHUNATH MURDESHWAR
Consultant Physician · Reg: 8052
Last reviewed: September 7, 2026
Overview
To detect anti-nuclear antibodies (ANAs) in the blood and, if positive, reflex to an ENA profile to identify specific autoantibodies associated with autoimmune diseases such as lupus, scleroderma, and mixed connective tissue disease. This aids in diagnosis, monitoring disease activity, and guiding treatment decisions.
- Test Code
- 147
- Price
- ₹4,500
- Sample Type
- Serum
- Result Time
- Reports are typically available the next working day after sample collection.
- Fasting Required
- Yes (12 hours)
- Method
- Immunofluorescence Assay (Hep-2 Substrate), Immunoblot
Sample Collection
Overnight fasting for 12 hours is preferred. Avoid strenuous activity before sample collection.
Method: Venipuncture
Laboratory Analysis
A blood sample will be drawn from a vein in your arm by a trained phlebotomist. The process is quick and minimally invasive.
Report Delivery
Apply pressure to the puncture site to prevent bruising. Resume normal activities unless advised otherwise.
Timeline: Reports are typically available the next working day after sample collection.
Patient Instructions
About This Test
Who Should Get This Test
To detect anti-nuclear antibodies (ANAs) in the blood and, if positive, reflex to an ENA profile to identify specific autoantibodies associated with autoimmune diseases such as lupus, scleroderma, and mixed connective tissue disease. This aids in diagnosis, monitoring disease activity, and guiding treatment decisions.
How to Prepare
- Overnight fasting preferred
- Sample type: serum from SST tube
- Ship refrigerated or frozen
- Provide complete clinical history to physician
Doctor's Notes
Reviewed by Dr SHAILAJA RAGHUNATH MURDESHWAR — MBBS, MD (General Medicine) · Reg. No. 8052
"This test is essential for diagnosing autoimmune conditions and should be interpreted alongside clinical findings for accurate patient management."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed or lipemic samples
- Incorrect sample volume
- Improper labeling or storage
- Contaminated samples
Understanding Your Results
ANA Negative
No detectable anti-nuclear antibodies; less likely for autoimmune disease, but does not completely rule it out.
ANA Positive with specific ENA antibodies
Suggestive of specific autoimmune conditions; e.g., anti-dsDNA for lupus, anti-Sm for lupus, anti-SSA/SSB for Sjögren's syndrome.
ANA Positive without specific ENA antibodies
May indicate early autoimmune disease or other conditions; requires further evaluation and monitoring.
If you experience persistent symptoms such as unexplained fatigue, joint pain, skin rashes, or if your test results are positive, consult a rheumatologist or immunologist for further evaluation and management.
Limitations
- ⚠Not solely diagnostic; requires clinical correlation
- ⚠False positives can occur in healthy individuals, especially elderly
- ⚠Does not specify disease activity or severity
- ⚠May not detect all autoimmune conditions
- ⚠Results should be interpreted by a qualified physician
Risks & Considerations
- ●Minor bruising or bleeding at puncture site
- ●Rare infection risk
- ●Faintness or dizziness during blood draw
Interfering Factors
- ●Certain medications (e.g., hydralazine, isoniazid)
- ●Recent infections
- ●Vaccinations
- ●Other autoimmune conditions
- ●Sample hemolysis or lipemia
Compare With Similar Tests
| Test | Anti Nuclear Antibody / Factor (ANA / ANF) IFA (HEP-2) Reflex to ENA Profile Test | Rheumatoid Factor (RF) | Anti-dsDNA Antibody | Erythrocyte Sedimentation Rate (ESR) | C-Reactive Protein (CRP) | Complement Levels (C3, C4) |
|---|---|---|---|---|---|---|
| Comparison | Anti Nuclear Antibody / Factor (ANA / ANF) IFA (HEP-2) Reflex to ENA Profile Test |
Frequently Asked Questions
What is the ANA/ANF IFA test?
Why is the ENA profile reflexed if ANA is positive?
What symptoms may prompt this test?
Is fasting required for this test?
How is the sample collected?
What does a positive ANA result mean?
Can ANA be positive in healthy individuals?
How accurate is the ANA/ANF IFA test?
What are the limitations of this test?
How long does it take to get the results?
Is the test covered by insurance?
How can I prepare for the test?
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₹4,500Reference Laboratory Services
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