DHEA Stimulation by ACTH Test
Short Name: DHEA Stimulation Test
Also known as: ACTH Stimulation Test for DHEA, DHEA Response Test
DHEA Stimulation by ACTH Test test available at DNA Labs India for ₹7,000. Uses Enzyme Immunoassay on Serum samples. Results in Same day report delivery via online portal, email, or WhatsApp.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Ramarao Paidisetty
Consultant Biochemist · Reg: 21504
Last reviewed: September 7, 2026
Overview
To diagnose DHEA deficiency and adrenal gland disorders by evaluating DHEA response to ACTH stimulation.
- Test Code
- 489
- Price
- ₹7,000
- Sample Type
- Serum
- Result Time
- Same day report delivery via online portal, email, or WhatsApp.
- Fasting Required
- Yes (10 hours)
- Method
- Enzyme Immunoassay
Sample Collection
Overnight fasting is mandatory (10-12 hours). Avoid strenuous activity and certain medications as advised by your doctor.
Method: Venipuncture
Laboratory Analysis
A baseline blood sample is drawn, followed by intravenous administration of 250 µg synthetic ACTH (Synacthen). A second blood sample is drawn 90 minutes later.
Report Delivery
Apply pressure to the injection site to prevent bleeding. Resume normal activities unless otherwise directed.
Timeline: Same day report delivery via online portal, email, or WhatsApp.
Patient Instructions
About This Test
Who Should Get This Test
To diagnose DHEA deficiency and adrenal gland disorders by evaluating DHEA response to ACTH stimulation.
How to Prepare
- Draw fasting baseline sample
- Administer 250 µg of synthetic ACTH (Synacthen) IV
- Draw additional sample at 90 minutes
- Ship refrigerated or frozen
- Both tubes must be clearly marked with time drawn
Doctor's Notes
Reviewed by Dr Ramarao Paidisetty — MBBS, MD (Biochemistry) · Reg. No. 21504
"The DHEA Stimulation by ACTH Test is crucial for evaluating adrenal function and diagnosing conditions like adrenal insufficiency or hyperplasia. Timely testing can guide effective treatment plans."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed or lipemic samples
- Incorrect labeling or missing time marks
- Sample not stored at recommended temperature
Understanding Your Results
Normal DHEA stimulation
Adrenal glands produce sufficient DHEA in response to ACTH
Blunted DHEA response
Possible primary or secondary adrenal insufficiency
Exaggerated DHEA response
May indicate adrenal hyperplasia or enzyme deficiencies
Consult a doctor if you experience symptoms of DHEA deficiency, such as chronic fatigue, low libido, or muscle weakness, or if test results indicate abnormal DHEA levels.
Limitations
- ⚠Does not assess other adrenal hormones like cortisol directly
- ⚠Results may vary with individual adrenal reserve
- ⚠Not suitable for patients with known hypersensitivity to ACTH
Risks & Considerations
- ●Minor pain or bruising at the injection site
- ●Rare allergic reaction to synthetic ACTH
- ●Dizziness or lightheadedness
Interfering Factors
- ●Medications such as corticosteroids or hormone therapies
- ●Recent physical or emotional stress
- ●Hepatic or renal impairment
- ●Inaccurate sample timing
Compare With Similar Tests
| Test | DHEA Stimulation by ACTH Test | DHEA-S Test | Cortisol Stimulation Test |
|---|---|---|---|
| Comparison | DHEA Stimulation by ACTH Test | Measures DHEA sulfate, a more stable form, without ACTH stimulation | Assesses cortisol response to ACTH, focusing on different adrenal hormone |
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