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DNA Labs India

E2A (ALL) Test

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E2A (ALL) Test

Also known as: E2A gene rearrangement, TCF3-PBX1 FISH, t(1;19) FISH

E2A (ALL) Test test available at DNA Labs India for ₹5,250. Uses FISH (Fluorescence In Situ Hybridization) on Bone Marrow / Peripheral Blood samples. Results in Results are typically available within 3-4 days after the sample reaches the laboratory.. Free home collection in 300+ cities across India.

FISH🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr Pasupathy Arumugam

Dr Pasupathy Arumugam

Consultant Pathologist · Reg: 21521

Last reviewed: September 7, 2026

Overview

The purpose of the E2A (ALL) test is to detect the presence of the t(1;19) translocation involving the E2A (TCF3) and PBX1 genes, which is a recurrent chromosomal abnormality in B-cell acute lymphoblastic leukemia. This test aids in diagnosis, risk stratification, and treatment planning. Identifying this translocation helps oncologists determine the appropriate chemotherapy regimen, as patients with E2A-PBX1 often have a good prognosis with intensive treatment. Additionally, the test is used for monitoring residual disease after therapy and for early detection of relapse.

Test Code
6095
CPT Code
88271
ICD Code
C91.0
Price
₹5,250
Sample Type
Bone Marrow / Peripheral Blood
Result Time
Results are typically available within 3-4 days after the sample reaches the laboratory.
Fasting Required
No
Method
FISH (Fluorescence In Situ Hybridization)
Step 1

Sample Collection

No special preparation is required. A doctor's prescription is mandatory. Inform your physician about any ongoing medications or recent treatments.

Method: Venipuncture or Bone Marrow Aspiration

Step 2

Laboratory Analysis

The sample (blood or bone marrow) will be collected by a trained phlebotomist or clinician. For bone marrow, a local anesthetic may be applied. The procedure is generally safe and takes a few minutes.

Step 3

Report Delivery

You may resume normal activities immediately. If a bone marrow biopsy was performed, keep the site clean and dry. Mild soreness is normal; contact your doctor if you experience excessive bleeding or signs of infection.

Timeline: Results are typically available within 3-4 days after the sample reaches the laboratory.

Patient Instructions

1
Before the Test:No special preparation. Ensure you have a valid doctor's prescription. Inform the lab about any recent chemotherapy or blood transfusions.
2
During the Test:A blood sample will be drawn from a vein in your arm, or a bone marrow biopsy will be performed. The procedure is quick and may cause minor discomfort.
3
After the Test:You can resume normal activities. For bone marrow biopsy, follow post-procedure care instructions provided by your doctor.

About This Test

Who Should Get This Test

The purpose of the E2A (ALL) test is to detect the presence of the t(1;19) translocation involving the E2A (TCF3) and PBX1 genes, which is a recurrent chromosomal abnormality in B-cell acute lymphoblastic leukemia. This test aids in diagnosis, risk stratification, and treatment planning. Identifying this translocation helps oncologists determine the appropriate chemotherapy regimen, as patients with E2A-PBX1 often have a good prognosis with intensive treatment. Additionally, the test is used for monitoring residual disease after therapy and for early detection of relapse.

How to Prepare

  • Ensure the sample is collected in a Sodium Heparin Vacutainer.
  • For bone marrow, the sample should be collected by an experienced oncologist.
  • Label the sample with patient details and date/time of collection.
  • Transport the sample to the laboratory at ambient temperature (15-25°C) within 24 hours.

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"Early detection of E2A-PBX1 translocation is critical for risk stratification in ALL. This FISH test provides rapid and accurate results, guiding targeted therapy decisions."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeBone Marrow / Peripheral Blood
Sample Volume2 ml
ContainerSodium Heparin Vacutainer
Collection MethodVenipuncture or Bone Marrow Aspiration

Sample Stability

Whole blood: 24 hours at room temperature
Bone marrow: 24-48 hours at room temperature
Do not freeze the sample.
Sample Rejection Criteria:
  • Hemolyzed or clotted sample
  • Incorrect container (e.g., EDTA instead of heparin)
  • Sample received after 48 hours
  • Insufficient volume

Understanding Your Results

The E2A (ALL) FISH test detects the t(1;19) translocation. A positive result indicates the presence of the E2A-PBX1 fusion gene, which is associated with a favorable prognosis in pediatric ALL when treated with intensive chemotherapy. A negative result suggests the absence of this specific translocation, but other genetic abnormalities may still be present.
📊

Positive for E2A-PBX1

Confirms the presence of t(1;19) translocation. Associated with pre-B cell ALL. Prognosis is generally good with intensive chemotherapy. Further risk stratification and treatment planning required.

📊

Negative for E2A-PBX1

No t(1;19) translocation detected. Other genetic abnormalities may be present. Additional cytogenetic/molecular testing may be needed to guide therapy.

⚠️ When to Consult a Doctor:

If you experience persistent symptoms such as fever, fatigue, unexplained bruising, or bone pain, consult a physician or oncologist promptly. Early diagnosis and treatment significantly improve outcomes in ALL.

Limitations

  • ⚠FISH only detects specific known translocations; other genetic abnormalities may be missed.
  • ⚠Low-level disease (minimal residual disease) may be below the detection threshold.
  • ⚠Results should be interpreted in conjunction with morphology and other cytogenetic/molecular tests.
  • ⚠Bone marrow sample is preferred over peripheral blood for higher sensitivity.

Risks & Considerations

  • ●Minimal risk of bleeding or infection at the collection site
  • ●Bone marrow biopsy may cause temporary pain or soreness
  • ●Rare risk of nerve damage (very low)

Interfering Factors

  • ●Recent blood transfusion may dilute abnormal cells, affecting sensitivity.
  • ●Hemolyzed or clotted samples may yield suboptimal results.
  • ●Insufficient sample volume can lead to inconclusive results.
  • ●Prior chemotherapy may reduce leukemic cell burden, potentially causing false negatives.

Compare With Similar Tests

TestE2A (ALL)BCR-ABL FISHMLL (KMT2A) FISHTEL-AML1 FISH
ComparisonE2A (ALL)Detects t(9;22) (Philadelphia chromosome) in CML/ALL. E2A-PBX1 is specific for t(1;19) in ALL. Both are FISH-based but target different translocations.Detects 11q23 rearrangements in ALL/AML. E2A-PBX1 is a distinct translocation; both are used for risk stratification.Detects t(12;21) in pediatric ALL. E2A-PBX1 is another recurrent translocation; both have prognostic implications.

Frequently Asked Questions

What is the cost of the E2A (ALL) test at DNA Labs India?
The E2A (ALL) test costs INR 5,250. This includes home sample collection and report delivery via email/WhatsApp.
What sample is required for the E2A (ALL) test?
The test can be performed on bone marrow or peripheral blood. A 2 ml sample in a Sodium Heparin Vacutainer is required.
Is fasting required before the E2A (ALL) test?
No, fasting is not required for this test.
How long does it take to get the E2A (ALL) test results?
Results are typically available within 3-4 days after the sample is received by the laboratory.
Is a doctor's prescription necessary for the E2A (ALL) test?
Yes, a doctor's prescription is required. However, it is not applicable for surgery or pregnancy cases or for those planning to travel abroad.
What does the E2A (ALL) test detect?
It detects the t(1;19) translocation involving the E2A (TCF3) and PBX1 genes, which is associated with acute lymphoblastic leukemia.
Is home sample collection available for the E2A (ALL) test?
Yes, DNA Labs India offers free home sample collection for online bookings across major cities in India.
What is the significance of a positive E2A-PBX1 result?
A positive result indicates the presence of the E2A-PBX1 fusion gene, which is associated with a favorable prognosis in pediatric ALL when treated with intensive chemotherapy.
Can the E2A (ALL) test be done on children?
Yes, the test is commonly performed on children, as ALL is more prevalent in this age group.
What is the method used for the E2A (ALL) test?
The test uses Fluorescence In Situ Hybridization (FISH) to detect the specific chromosomal translocation.
Are there any risks associated with the bone marrow biopsy?
Bone marrow biopsy is generally safe but may cause temporary pain, bleeding, or infection at the site. Serious complications are rare.
How should I book the E2A (ALL) test?
You can book online through the DNA Labs India website or contact our customer care. A doctor's prescription is required.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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