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Epidermal Growth Factor Receptor Mutation Analysis (EGFR - Exon 18, 19, 20, 21) Test

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Epidermal Growth Factor Receptor Mutation Analysis (EGFR - Exon 18, 19, 20, 21) Test

Short Name: EGFR Mutation Analysis

Also known as: EGFR Mutation Panel, EGFR Exon 18-21 Analysis, EGFR Gene Mutation Test

Epidermal Growth Factor Receptor Mutation Analysis (EGFR - Exon 18, 19, 20, 21) Test test available at DNA Labs India for ₹10,500. Uses Sanger Sequencing on Tumor tissue (paraffin-embedded block) samples. Results in Reports are delivered within 3-4 days after the sample is received at the laboratory.. Free home collection in 300+ cities across India.

GeneticAdult🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr Pasupathy Arumugam

Dr Pasupathy Arumugam

Consultant Pathologist · Reg: 21521

Last reviewed: September 7, 2026

Overview

The purpose of EGFR mutation analysis is to identify specific genetic alterations in the EGFR gene that can guide treatment decisions in NSCLC. It helps determine whether a patient is likely to benefit from EGFR-targeted therapies, which are more effective and less toxic than conventional chemotherapy. Additionally, it aids in detecting resistance mutations that may emerge during treatment, allowing for timely adjustment of therapy.

Test Code
6100
CPT Code
81235
ICD Code
C34.90
Price
₹10,500
Sample Type
Tumor tissue (paraffin-embedded block)
Result Time
Reports are delivered within 3-4 days after the sample is received at the laboratory.
Fasting Required
No
Method
Sanger Sequencing
Step 1

Sample Collection

No special preparation is required. A doctor's prescription is necessary. Inform your doctor about any medications you are taking.

Method: Biopsy or surgical resection

Step 2

Laboratory Analysis

The sample is a tumor tissue block or slides; no blood draw is involved. The procedure is performed during biopsy or surgery.

Step 3

Report Delivery

No specific aftercare is needed. The tissue sample is sent to the laboratory for analysis.

Timeline: Reports are delivered within 3-4 days after the sample is received at the laboratory.

Patient Instructions

1
Before the Test:No special preparation is needed. Ensure you have a doctor's prescription and provide relevant clinical history.
2
During the Test:The test is performed on a tissue sample; no pain or discomfort is involved.
3
After the Test:You can resume normal activities immediately. Results are typically available in 3-4 days.

About This Test

Who Should Get This Test

The purpose of EGFR mutation analysis is to identify specific genetic alterations in the EGFR gene that can guide treatment decisions in NSCLC. It helps determine whether a patient is likely to benefit from EGFR-targeted therapies, which are more effective and less toxic than conventional chemotherapy. Additionally, it aids in detecting resistance mutations that may emerge during treatment, allowing for timely adjustment of therapy.

How to Prepare

  • Tissue sample must be fixed in 10% neutral buffered formalin for 6-48 hours
  • Paraffin-embedded tissue block or 5-10 unstained sections (4-5 µm thick) should be provided
  • Sample must be accompanied by a pathology report and clinical history
  • Ensure sample is properly labeled with patient identification

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"EGFR mutation testing is essential for guiding targeted therapy in NSCLC. Early detection of these mutations can significantly improve treatment outcomes."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeTumor tissue (paraffin-embedded block)
Sample VolumeNot applicable (tissue block)
ContainerParaffin block or unstained slides
Collection MethodBiopsy or surgical resection

Sample Stability

Paraffin-embedded tissue: stable at room temperature for years
Unstained slides: stable for up to 2 weeks at room temperature
DNA extracted: stable at -20°C for up to 1 year
Sample Rejection Criteria:
  • Inadequate tumor content (<20%)
  • Sample not fixed properly or over-fixed (>48 hours)
  • Decalcified bone specimens
  • Insufficient tissue quantity
  • Sample without proper labeling or requisition form

Understanding Your Results

The EGFR mutation analysis report indicates the presence or absence of mutations in exons 18, 19, 20, and 21. The interpretation should be done by an oncologist in the context of the patient's clinical condition.
📊

Sensitizing mutations; patient likely to benefit from EGFR TKIs (e.g., erlotinib, gefitinib, osimertinib)

📊

Resistance mutation; consider third-generation TKI (osimertinib) or alternative therapy

📊

Generally resistant to first-generation TKIs; may respond to newer agents or chemotherapy

📊

Rare sensitizing mutation; may respond to TKIs but with variable efficacy

📊

No actionable EGFR mutation; consider testing for other biomarkers (ALK, ROS1, BRAF, etc.)

⚠️ When to Consult a Doctor:

Consult your oncologist if you have been diagnosed with NSCLC and are considering targeted therapy. Also, if you experience new or worsening symptoms such as persistent cough, chest pain, shortness of breath, or unexplained weight loss, seek medical advice.

Limitations

  • ⚠Sanger sequencing has a sensitivity of approximately 20% mutant allele frequency; low-level mutations may be missed
  • ⚠Only detects mutations in exons 18-21; other rare EGFR mutations may not be covered
  • ⚠Requires adequate tumor tissue; not suitable for cytology samples without cell block
  • ⚠Cannot detect large deletions or rearrangements involving EGFR
  • ⚠Results should be interpreted in conjunction with clinical and pathological findings

Risks & Considerations

  • ●No direct risks from the test itself as it is performed on an existing tissue sample
  • ●Risks are associated with the biopsy procedure, which are discussed with your doctor

Interfering Factors

  • ●Insufficient tumor content in the sample (<20%) may lead to false-negative results
  • ●Poor DNA quality due to prolonged formalin fixation
  • ●Decalcification of bone specimens can degrade DNA
  • ●Concurrent use of EGFR inhibitors may affect mutation detection in circulating tumor DNA (if applicable)

Compare With Similar Tests

TestEpidermal Growth Factor Receptor Mutation Analysis (EGFR - Exon 18, 19, 20, 21)
ComparisonEpidermal Growth Factor Receptor Mutation Analysis (EGFR - Exon 18, 19, 20, 21)

Frequently Asked Questions

What is EGFR mutation analysis?
EGFR mutation analysis is a genetic test that detects mutations in the EGFR gene, commonly found in non-small cell lung cancer (NSCLC). It helps guide targeted therapy decisions.
Which exons are tested in this analysis?
The test covers exons 18, 19, 20, and 21 of the EGFR gene, which are the most common mutation hotspots.
What is the cost of the test?
The cost is INR 10500, which includes home sample collection and report delivery.
What sample is required?
Tumor tissue (paraffin-embedded block or unstained slides) obtained from biopsy or surgery.
Do I need to fast before the test?
No, fasting is not required for this test.
How long does it take to get results?
Reports are typically available within 3-4 days after the sample is received.
What do the results mean?
Positive results for sensitizing mutations (exon 19 deletion, L858R) indicate likely benefit from EGFR TKIs. Resistance mutations like T790M guide alternative therapy.
Is home sample collection available?
Yes, we offer free home sample collection for online bookings across major cities in India.
Can the test be done on blood?
This test is performed on tumor tissue. However, liquid biopsy (blood) for EGFR mutations is also available separately.
Who should get this test?
Patients diagnosed with NSCLC, especially adenocarcinoma, who are candidates for targeted therapy.
Is a doctor's prescription required?
Yes, a doctor's prescription is required for this test.
What is the sensitivity of Sanger sequencing?
Sanger sequencing has a sensitivity of approximately 20% mutant allele frequency, meaning it may miss low-level mutations.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

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✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

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