Epidermal (Pemphigus) Antibody IFA in Dilutions Test
Also known as: Pemphigus Antibody IFA Test, Epidermal Antibody Dilutions Test
Epidermal (Pemphigus) Antibody IFA in Dilutions Test test available at DNA Labs India for ₹5,500. Uses Immunofluorescence Assay (IFA) on Serum samples. Results in Reports are typically available the next day after sample collection.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
To detect and quantify autoantibodies against epidermal cells for the diagnosis and monitoring of pemphigus vulgaris, an autoimmune blistering skin disease.
- Test Code
- 518
- Price
- ₹5,500
- Sample Type
- Serum
- Result Time
- Reports are typically available the next day after sample collection.
- Fasting Required
- Yes
- Method
- Immunofluorescence Assay (IFA)
Sample Collection
Overnight fasting (8-12 hours) is preferred. Inform the healthcare provider about any medications or recent transfusions.
Method: Venipuncture
Laboratory Analysis
A blood sample will be drawn from a vein in your arm using standard venipuncture techniques. The process is quick and minimally invasive.
Report Delivery
Apply pressure to the puncture site to prevent bruising. Resume normal activities unless otherwise advised.
Timeline: Reports are typically available the next day after sample collection.
Patient Instructions
About This Test
Who Should Get This Test
To detect and quantify autoantibodies against epidermal cells for the diagnosis and monitoring of pemphigus vulgaris, an autoimmune blistering skin disease.
How to Prepare
- Fast for 8-12 hours before the test, preferably overnight
- Wear comfortable clothing with easy access to the arm
- Stay hydrated by drinking water, but avoid eating
- Bring identification and any relevant medical records
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"This test is crucial for diagnosing pemphigus vulgaris, an autoimmune blistering disorder. Early detection via antibody testing supports timely intervention and management by dermatologists or immunologists."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed, lipemic, or insufficient sample volume
- Improperly labeled or unlabeled specimens
- Samples collected in incorrect containers
- Specimens exceeding stability limits
Understanding Your Results
Positive (e.g., titer ≥1:10)
Indicates presence of antibodies consistent with pemphigus vulgaris. Further evaluation by a dermatologist or immunologist is recommended.
Negative
No detectable antibodies. Pemphigus vulgaris is less likely, but other conditions should be considered if symptoms persist.
Equivocal or low titer
May require repeat testing or additional diagnostic workup, such as biopsy, for confirmation.
Consult a dermatologist or immunologist if you experience persistent blistering, oral erosions, or if test results are abnormal or inconsistent with symptoms.
Limitations
- ⚠False positives can occur due to cross-reactivity with other autoimmune conditions
- ⚠False negatives may arise in early disease or with low antibody levels
- ⚠Test does not distinguish between pemphigus subtypes (e.g., vulgaris vs. foliaceus)
- ⚠Results should be correlated with clinical findings and other diagnostic tests like biopsy
Risks & Considerations
- ●Minor pain or bruising at the blood draw site
- ●Rarely, infection or fainting
- ●No significant risks associated with the test itself
Interfering Factors
- ●Hemolysis or lipemia in the serum sample
- ●Recent blood transfusions or immunotherapy
- ●Use of immunosuppressive medications
- ●Improper sample handling or storage
Compare With Similar Tests
| Test | Epidermal (Pemphigus) Antibody IFA in Dilutions Test | Direct Immunofluorescence (DIF) | Skin Biopsy | Anti-Desmoglein Antibodies ELISA | Indirect Immunofluorescence (IIF) |
|---|---|---|---|---|---|
| Comparison | Epidermal (Pemphigus) Antibody IFA in Dilutions Test |
Frequently Asked Questions
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