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Epidermal (Pemphigus) Antibody IFA Test

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Epidermal (Pemphigus) Antibody IFA Test

Short Name: Pemphigus Antibody IFA Test

Also known as: Epidermal Antibody Test, Pemphigus Antibody Test, IFA Test for Pemphigus

Epidermal (Pemphigus) Antibody IFA Test test available at DNA Labs India for ₹3,500. Uses Immunofluorescence Assay on Serum samples. Results in Reports are available on the same day if the sample is received by 11 am.. Free home collection in 300+ cities across India.

Laboratory Test🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr Pasupathy Arumugam

Dr Pasupathy Arumugam

Consultant Pathologist · Reg: 21521

Last reviewed: September 7, 2026

Overview

To detect autoantibodies against desmoglein proteins for the diagnosis of pemphigus vulgaris and pemphigus foliaceus, aiding in early management and treatment of autoimmune blistering diseases.

Test Code
527
Price
₹3,500
Sample Type
Serum
Result Time
Reports are available on the same day if the sample is received by 11 am.
Fasting Required
Yes
Method
Immunofluorescence Assay
Step 1

Sample Collection

Overnight fasting is preferred.

Method: Venipuncture

Step 2

Laboratory Analysis

Your sample is analyzed using Immunofluorescence Assay in our laboratory.

Step 3

Report Delivery

A certified pathologist reviews and signs your report. You receive it as a secure PDF via email and WhatsApp.

Timeline: Reports are available on the same day if the sample is received by 11 am.

Patient Instructions

1
Before the Test:Overnight fasting is preferred. Inform your doctor about any medications.
2
During the Test:A blood sample will be collected from a vein in your arm. The process is quick and minimally invasive.
3
After the Test:Apply pressure to the puncture site. You can resume normal activities immediately.

About This Test

Who Should Get This Test

To detect autoantibodies against desmoglein proteins for the diagnosis of pemphigus vulgaris and pemphigus foliaceus, aiding in early management and treatment of autoimmune blistering diseases.

How to Prepare

  • Collect 2 mL serum via venipuncture into a Serum Separator Tube (SST).
  • Ship the sample refrigerated or frozen.

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"This test is essential for diagnosing pemphigus, a condition that requires early intervention to prevent complications such as skin infections and nutritional deficiencies."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeSerum
Sample Volume2 mL (1 mL min.)
ContainerSST (Serum Separator Tube)
Collection MethodVenipuncture

Sample Stability

Room Temperature
Refrigerator
Frozen

Understanding Your Results

The Epidermal (Pemphigus) Antibody IFA test detects the presence of autoantibodies associated with pemphigus. Results are interpreted as positive or negative.
📊

Positive

Indicates the presence of autoantibodies against desmoglein, suggesting pemphigus vulgaris or pemphigus foliaceus. Clinical correlation is recommended.

📊

Negative

No detectable autoantibodies. Pemphigus is less likely, but if clinical suspicion remains, additional tests may be needed.

⚠️ When to Consult a Doctor:

If you experience symptoms such as blistering skin lesions, mucous membrane erosions, or difficulty eating, consult a dermatologist or immunologist promptly.

Risks & Considerations

  • ●Minor bruising at the needle site
  • ●Slight pain during blood draw
  • ●Rare chance of infection

Compare With Similar Tests

TestEpidermal (Pemphigus) Antibody IFA TestDirect Immunofluorescence (DIF) TestIndirect Immunofluorescence (IDIF) TestELISA for Desmoglein AntibodiesSkin Biopsy
ComparisonEpidermal (Pemphigus) Antibody IFA TestPerformed on a skin biopsy to detect antibody deposits directly in the skin.Similar to IFA but uses patient serum to detect circulating antibodies.Quantitative test to measure specific antibody levels.Histopathological examination to identify blister formation and inflammation.

Frequently Asked Questions

What is the Epidermal (Pemphigus) Antibody IFA Test?
This is a blood test that detects autoantibodies against desmoglein proteins, used to diagnose pemphigus vulgaris and pemphigus foliaceus.
What are the symptoms that indicate the need for this test?
Symptoms include blistering skin lesions, painful erosions, difficulty swallowing, fatigue, fever, and weight loss.
How is the test performed?
A blood sample is collected via venipuncture and analyzed using immunofluorescence assay to detect specific antibodies.
What is the cost of the test?
The cost is INR 3500, which includes free home sample collection across India.
Is fasting required for the test?
Overnight fasting is preferred for accurate results.
How long does it take to get the results?
Reports are typically available on the same day if the sample is received by 11 am.
What does a positive result mean?
A positive result indicates the presence of autoantibodies, suggesting pemphigus. Clinical correlation is necessary for confirmation.
Are there any risks associated with the test?
The test is safe with minimal risks such as minor bruising or discomfort at the needle site.
How accurate is the Epidermal (Pemphigus) Antibody IFA Test?
It is a reliable test for detecting pemphigus antibodies, but should be interpreted along with clinical findings.
Can the test be done at home?
Yes, DNA Labs India offers free home sample collection for this test.
What other tests are used to diagnose pemphigus?
Other tests include Direct Immunofluorescence (DIF), Indirect Immunofluorescence (IDIF), ELISA, and skin biopsy.
Where can I get this test done?
You can book the test at DNA Labs India with home collection available in numerous cities across India.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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