FISH - 17p (TP53) Deletion Test
Short Name: FISH 17p Deletion
Also known as: FISH 17p Deletion Test, TP53 Gene Deletion Test, Chromosome 17p Deletion FISH, TP53 FISH Analysis, 17p13.1 Deletion Test
FISH - 17p (TP53) Deletion Test test available at DNA Labs India for ₹6,500. Uses Fluorescence In Situ Hybridization (FISH) on Whole Blood or Bone Marrow Aspirate samples. Results in Sample accepted daily by 4:00 PM. Report available within 4 working days from the date of sample receipt at the laboratory.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
The primary purpose of the FISH 17p (TP53) Deletion Test is to detect the deletion of the TP53 tumour-suppressor gene located on chromosome 17p13.1. This information is critical for prognosis assessment, risk stratification, and treatment planning in patients with multiple myeloma and other haematological malignancies. It helps clinicians determine whether a patient carries a high-risk cytogenetic abnormality that may necessitate more aggressive therapy or alternative treatment approaches.
- Test Code
- 567
- CPT Code
- 88271
- ICD Code
- C90.0
- Price
- ₹6,500
- Sample Type
- Whole Blood or Bone Marrow Aspirate
- Result Time
- Sample accepted daily by 4:00 PM. Report available within 4 working days from the date of sample receipt at the laboratory.
- Fasting Required
- No
- Method
- Fluorescence In Situ Hybridization (FISH)
Sample Collection
No specific patient preparation such as fasting is required. Ensure the Chromosome & FISH analysis Requisition Form (Form 17) is duly filled and accompanies the sample. Inform the clinician about any anticoagulant therapy. The sample must be collected aseptically and shipped at 18-22°C without freezing.
Method: Venipuncture (blood) / Bone marrow aspiration (marrow)
Laboratory Analysis
For blood: Collect 5 mL (minimum 3 mL) of whole blood via standard venipuncture into 2 Green Top (Sodium Heparin) tubes. Invert gently 8-10 times to mix. For bone marrow: Aspirate 4 mL (minimum 2 mL) of bone marrow into Sodium Heparin tubes. Label tubes clearly with patient details. Complete Form 17 in full.
Report Delivery
Transport the sample at room temperature (18-22°C) to the laboratory within 48 hours. Do not refrigerate or freeze the sample. Results will be available within 4 working days from sample receipt. Reports can be accessed via the online portal, email, or WhatsApp.
Timeline: Sample accepted daily by 4:00 PM. Report available within 4 working days from the date of sample receipt at the laboratory.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of the FISH 17p (TP53) Deletion Test is to detect the deletion of the TP53 tumour-suppressor gene located on chromosome 17p13.1. This information is critical for prognosis assessment, risk stratification, and treatment planning in patients with multiple myeloma and other haematological malignancies. It helps clinicians determine whether a patient carries a high-risk cytogenetic abnormality that may necessitate more aggressive therapy or alternative treatment approaches.
How to Prepare
- Collect 5 mL whole blood or 4 mL bone marrow aspirate in Sodium Heparin (Green Top) tubes
- Invert tubes gently 8-10 times immediately after collection
- Ship at 18-22°C; DO NOT FREEZE
- Ensure sample reaches the laboratory within 48 hours of collection
- Duly filled Chromosome & FISH analysis Requisition Form (Form 17) is mandatory
- Free home sample collection is available for online bookings across India
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"Deletion of the 17p region encompassing the TP53 gene is one of the most important adverse prognostic markers in multiple myeloma. Patients harbouring this deletion typically demonstrate shorter progression-free survival and overall survival. FISH-based detection of 17p deletion guides risk-stratified treatment decisions, including the consideration of more aggressive therapeutic regimens and early referral for stem-cell transplant evaluation. I recommend this test for all newly diagnosed multiple myeloma patients as part of standard cytogenetic workup."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Sample received frozen or at incorrect temperature
- Sample collected in EDTA tube instead of Sodium Heparin tube
- Sample volume below minimum requirement
- Sample received after 48 hours of collection at room temperature
- Missing or incomplete Chromosome & FISH analysis Requisition Form (Form 17)
- Haemolysed, clotted, or contaminated sample
- Unlabelled or mislabelled tubes
Understanding Your Results
Normal (No Deletion Detected)
Two intact TP53 signals observed in ≥90% of cells examined. The 17p region is intact, and no TP53 deletion is detected by FISH. In the context of multiple myeloma, the absence of 17p deletion is associated with a standard-risk cytogenetic profile. However, this does not exclude point mutations or other TP53 abnormalities not detectable by this assay.
Deletion Detected
Loss of one TP53 signal observed in ≥10% of interphase nuclei scored, confirming 17p deletion. In multiple myeloma, this is classified as a high-risk cytogenetic abnormality by the IMWG, associated with aggressive disease, shorter progression-free survival, and inferior overall survival. This finding may prompt escalation of therapy, consideration of novel agents, or early transplant referral.
Borderline / Inconclusive
TP53 signal loss observed in <10% of cells. This may represent low-level mosaicism, technical artefact, or borderline clonal population. Repeat testing on a fresh sample, correlation with conventional cytogenetics, or additional molecular testing (e.g., TP53 mutation analysis by NGS) may be recommended.
Consult your haemato-oncologist or treating physician if the FISH test reveals 17p deletion, as this indicates high-risk disease requiring specialised treatment planning. Also seek medical advice if results are inconclusive or if you have a family history suggestive of Li-Fraumeni syndrome. Any new symptoms such as unexplained bone pain, recurrent infections, fatigue, or unexplained weight loss should prompt immediate medical evaluation.
Limitations
- ⚠FISH detects numerical deletions at the targeted locus only; point mutations or structural rearrangements of TP53 will not be identified
- ⚠Low-level mosaicism below the detection threshold (~5-10%) may be missed
- ⚠Results must be correlated with clinical findings, conventional cytogenetics, and other molecular tests
- ⚠This test does not assess the functional status of the remaining TP53 allele
- ⚠Bone marrow aspirate provides higher tumour cell yield than peripheral blood in leukaemic patients
Risks & Considerations
- ●For blood draw: Minor bruising, slight pain at the venipuncture site, or rare vasovagal reaction
- ●For bone marrow aspiration: Local pain at the aspiration site, minor bleeding, and very rare risk of infection at the procedure site
- ●Psychological impact of receiving a high-risk cytogenetic result; genetic counselling is advised
Interfering Factors
- ●Sample haemolysis or clotting may compromise cell integrity
- ●Improper storage temperature (sample must be shipped at 18-22°C; do not freeze)
- ●Insufficient sample volume below minimum requirements
- ●Prolonged transit time beyond 48 hours at room temperature
- ●Contamination or mix-up of sample tubes
- ●Failure to submit the mandatory Chromosome & FISH analysis Requisition Form (Form 17)
Compare With Similar Tests
| Test | FISH - 17p (TP53) Deletion Test | ||||
|---|---|---|---|---|---|
| Comparison | FISH - 17p (TP53) Deletion Test |
Frequently Asked Questions
What is the FISH 17p (TP53) Deletion Test?
Why is the FISH 17p TP53 Deletion Test important in multiple myeloma?
What sample is required for the FISH 17p TP53 Deletion Test?
How much does the FISH 17p TP53 Deletion Test cost at DNA Labs India?
Is fasting required before the FISH 17p TP53 Deletion Test?
How long does it take to get the FISH 17p TP53 Deletion Test results?
Is the FISH 17p TP53 Deletion Test painful?
What does a positive (deletion detected) result mean?
Can the FISH 17p TP53 Deletion Test detect all types of TP53 mutations?
Is home sample collection available for this test?
What is the difference between FISH and conventional karyotyping for detecting 17p deletion?
Who should get the FISH 17p TP53 Deletion Test?
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