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DNA Labs India

FISH - EGFR Gene Amplification Test

DNA Labs India | ISO 9001:2015 Certified

FISH - EGFR Gene Amplification Test

Short Name: EGFR FISH Test

Also known as: EGFR FISH Test, Fluorescence In Situ Hybridization for EGFR

FISH - EGFR Gene Amplification Test test available at DNA Labs India for ₹12,000. Uses FISH on Formalin fixed paraffin embedded tissue block samples. Results in Report in 4 working days after sample receipt.. Free home collection in 300+ cities across India.

DiagnosticAdults🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr SHAILAJA RAGHUNATH MURDESHWAR

Dr SHAILAJA RAGHUNATH MURDESHWAR

Consultant Physician · Reg: 8052

Last reviewed: September 7, 2026

Overview

To diagnose EGFR gene amplification in cancer, especially non-small cell lung cancer (NSCLC), for targeted therapy selection and treatment planning.

Test Code
575
Price
₹12,000
Sample Type
Formalin fixed paraffin embedded tissue block
Result Time
Report in 4 working days after sample receipt.
Fasting Required
No
Method
FISH
Step 1

Sample Collection

No specific preparation needed. Inform doctor about any medications. Ensure biopsy or surgery is scheduled.

Method: Biopsy or Surgery

Step 2

Laboratory Analysis

Sample collected via biopsy or surgery. Tissue is preserved in formalin for fixation.

Step 3

Report Delivery

Sample is sent to the laboratory for processing and analysis.

Timeline: Report in 4 working days after sample receipt.

Patient Instructions

1
Before the Test:No special preparation needed. Ensure your doctor provides the requisition form and that the tissue sample is properly fixed and submitted.
2
During the Test:Not applicable; test is performed on tissue sample in the laboratory.
3
After the Test:Wait for report delivery. Consult your doctor for interpretation and next steps.

About This Test

Who Should Get This Test

To diagnose EGFR gene amplification in cancer, especially non-small cell lung cancer (NSCLC), for targeted therapy selection and treatment planning.

How to Prepare

  • Submit formalin-fixed paraffin-embedded tissue block.
  • Ship at room temperature.
  • Ensure tissue block contains at least 10% tumor tissue.
  • Duly filled Chromosome & FISH analysis Requisition Form (Form 17) is mandatory.

Doctor's Notes

Reviewed by — MBBS, MD (General Medicine) · Reg. No. 8052

"This test is crucial for identifying EGFR amplification in NSCLC patients, guiding targeted therapy decisions to improve treatment outcomes."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeFormalin fixed paraffin embedded tissue block
ContainerFormalin fixed paraffin embedded tissue block
Collection MethodBiopsy or Surgery

Sample Stability

Room temperature: Stable for shipping
Long-term storage: As per laboratory protocols
Sample Rejection Criteria:
  • Insufficient tumor tissue (<10%)
  • Improper fixation or preservation
  • Missing or incomplete requisition form

Understanding Your Results

The FISH - EGFR Gene Amplification Test detects amplification of the EGFR gene in tumor samples, which is common in non-small cell lung cancer and other cancers, aiding in treatment decisions.
Negative Result: No EGFR gene amplification detected. Suggests normal gene copy number. Patient may not benefit from EGFR-targeted therapies.
Positive Result: EGFR gene amplification detected. Indicates potential responsiveness to EGFR inhibitors such as erlotinib or gefitinib.
Borderline/Indeterminate: Requires further testing or repeat analysis for confirmation.
⚠️ When to Consult a Doctor:

If you have symptoms of lung cancer, have been diagnosed with NSCLC, or your doctor recommends genetic testing for treatment planning, consult an oncologist for this test.

Limitations

  • ⚠Detects only gene amplification, not point mutations or other alterations
  • ⚠Requires adequate tumor tissue for accurate results
  • ⚠Limited to formalin-fixed paraffin-embedded samples

Risks & Considerations

  • ●Risk from biopsy or surgery: infection, bleeding, or discomfort at the collection site.

Interfering Factors

  • ●Poor tissue fixation
  • ●Insufficient tumor content (<10%)
  • ●Technical errors in FISH procedure

Compare With Similar Tests

TestFISH - EGFR Gene Amplification TestEGFR Mutation AnalysisALK FISH TestPD-L1 Immunohistochemistry
ComparisonFISH - EGFR Gene Amplification TestDetects mutations in EGFR gene, not amplification.Tests for ALK gene rearrangements in lung cancer.Assesses PD-L1 expression for immunotherapy eligibility.

Frequently Asked Questions

What is the FISH - EGFR Gene Amplification Test?
It is a molecular test that uses Fluorescence In Situ Hybridization to detect amplification of the EGFR gene in tumor tissues, commonly used in non-small cell lung cancer diagnosis.
Why is this test important?
This test helps identify patients who may benefit from EGFR-targeted therapies, enabling personalized treatment plans and improving outcomes.
What symptoms might indicate the need for this test?
Symptoms such as persistent cough, chest pain, shortness of breath, wheezing, weight loss, and fatigue in the context of suspected lung cancer.
How is the sample for this test collected?
A tissue sample is collected via biopsy or surgery and fixed in formalin to create a paraffin-embedded block for analysis.
What is the cost of the FISH - EGFR Gene Amplification Test?
The test costs INR 12,000 at DNA Labs India, with free home sample collection available.
Is home collection available for this test?
Yes, DNA Labs India offers free home sample collection for this test across India.
How long does it take to get the report?
The report is typically delivered within 4 working days after sample receipt.
What does a positive result mean?
A positive result indicates EGFR gene amplification, suggesting that the patient may respond well to EGFR-targeted therapies.
What is the reference range for EGFR amplification?
The reference range is based on the EGFR/CEP7 ratio: less than 2.0 is normal, and 2.0 or greater indicates amplification.
Can this test be used for cancers other than lung cancer?
While primarily for NSCLC, EGFR amplification can occur in other cancers, but the test is optimized for lung cancer tissues.
Are there any risks associated with the test?
The test itself has no direct risks, but the biopsy or surgery for sample collection may have associated risks like infection or bleeding.
How do I prepare for this test?
No special preparation is needed. Ensure your doctor provides the requisition form and that the tissue sample is properly fixed and submitted.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

LIMS Integration

Your Data Privacy

Your medical data is protected under Indian law.

✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

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