FISH - t(11;14) or LSI IGH/CCND1 Test
Short Name: FISH t(11;14)
Also known as: LSI IGH/CCND1 Test, t(11;14) FISH Assay, CCND1/IGH Fusion Test
FISH - t(11;14) or LSI IGH/CCND1 Test test available at DNA Labs India for ₹6,000. Uses Fluorescence In Situ Hybridization (FISH) on Whole Blood or Bone Marrow samples. Results in Reports are delivered within 4 working days from sample receipt. Same-day reports are not available for this test.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
The primary purpose of the FISH - t(11;14) test is to identify the t(11;14) translocation in suspected cases of mantle cell lymphoma or multiple myeloma. This genetic alteration leads to overexpression of the CCND1 gene, contributing to uncontrolled cell growth. By detecting this translocation, the test aids in confirming diagnoses, differentiating from other lymphomas, assessing prognosis, and guiding targeted therapy decisions, such as the use of CDK4/6 inhibitors.
- Test Code
- 604
- Price
- ₹6,000
- Sample Type
- Whole Blood or Bone Marrow
- Result Time
- Reports are delivered within 4 working days from sample receipt. Same-day reports are not available for this test.
- Fasting Required
- No
- Method
- Fluorescence In Situ Hybridization (FISH)
Sample Collection
No special preparation is required. Ensure the Chromosome & FISH analysis Requisition Form (Form 17) is duly filled. Inform the healthcare provider about any medications or conditions.
Method: Venipuncture or Bone Marrow Aspiration
Laboratory Analysis
For blood samples, a venipuncture will be performed. For bone marrow, aspiration from the hip bone under local anesthesia. The procedure takes 15-30 minutes with minimal discomfort.
Report Delivery
Apply pressure to the collection site to prevent bruising. Avoid strenuous activity for a few hours if bone marrow was aspirated. Resume normal activities unless advised otherwise.
Timeline: Reports are delivered within 4 working days from sample receipt. Same-day reports are not available for this test.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of the FISH - t(11;14) test is to identify the t(11;14) translocation in suspected cases of mantle cell lymphoma or multiple myeloma. This genetic alteration leads to overexpression of the CCND1 gene, contributing to uncontrolled cell growth. By detecting this translocation, the test aids in confirming diagnoses, differentiating from other lymphomas, assessing prognosis, and guiding targeted therapy decisions, such as the use of CDK4/6 inhibitors.
How to Prepare
- Collect 5 mL whole blood or 4 mL bone marrow in Sodium Heparin (Green Top) tubes.
- Ship samples at 18-22°C; do not freeze.
- Label tubes correctly with patient details and form number.
- Ensure the requisition form (Form 17) is submitted with the sample.
- Use aseptic technique to avoid contamination.
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"This FISH test is essential for diagnosing mantle cell lymphoma and certain cases of multiple myeloma, helping guide targeted treatment strategies for better patient outcomes."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Sample collected in wrong tube type (not Sodium Heparin)
- Hemolyzed or clotted sample
- Insufficient sample volume
- Missing or incomplete requisition form
- Sample stored at incorrect temperature (frozen or refrigerated)
Understanding Your Results
Negative
No t(11;14) translocation detected. This suggests absence of mantle cell lymphoma-related genetic alteration, but other diagnoses may still be considered.
Positive
t(11;14) translocation detected, supporting diagnosis of mantle cell lymphoma or certain multiple myeloma cases. Further clinical evaluation and treatment planning are recommended.
Consult an oncologist or hematologist if you experience symptoms like swollen lymph nodes, unexplained weight loss, or fatigue. After receiving test results, discuss them with your doctor for personalized management.
Limitations
- ⚠FISH detects only specific translocations; it may not identify all genetic abnormalities.
- ⚠Results are qualitative and require expert interpretation by a cytogeneticist.
- ⚠False negatives can occur if translocation is present in low proportion of cells.
- ⚠Test does not provide information on other mutations or disease stages.
Risks & Considerations
- ●Minimal risk of bruising or infection at the needle site
- ●Rare allergic reaction to local anesthesia (for bone marrow aspiration)
- ●Discomfort or soreness after bone marrow procedure
- ●False positives or negatives due to technical limitations
Interfering Factors
- ●Poor sample quality due to hemolysis or clotting
- ●Improper sample collection or storage (e.g., freezing)
- ●Contamination during processing
- ●Low cellularity in bone marrow samples
- ●Technical errors in probe hybridization
Compare With Similar Tests
| Test | FISH - t(11;14) or LSI IGH/CCND1 Test | Bone Marrow Biopsy | Flow Cytometry | PCR for IGH/CCND1 |
|---|---|---|---|---|
| Comparison | FISH - t(11;14) or LSI IGH/CCND1 Test | Biopsy provides histological assessment, while FISH identifies specific genetic changes. Both are complementary in lymphoma diagnosis. | Flow cytometry analyzes cell surface markers, whereas FISH detects chromosomal abnormalities. They are often used together for accurate classification. | PCR is more sensitive for detecting low-level translocations but requires DNA extraction. FISH preserves cellular context and is standard for initial screening. |
Frequently Asked Questions
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