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FISH - t(15;17) or LSI PML/RARA Test

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FISH - t(15;17) or LSI PML/RARA Test

Short Name: PML/RARA FISH Test

Also known as: PML/RARA FISH test, t(15;17) FISH test, FISH for PML-RARA

FISH - t(15;17) or LSI PML/RARA Test test available at DNA Labs India for ₹6,000. Uses FISH on Whole Blood or Bone Marrow samples. Results in 4 working days from sample receipt. Free home collection in 300+ cities across India.

Diagnostic🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr Pasupathy Arumugam

Dr Pasupathy Arumugam

Consultant Pathologist · Reg: 21521

Last reviewed: September 7, 2026

Overview

To diagnose Acute Promyelocytic Leukemia (APL) by detecting the PML/RARA gene fusion in bone marrow or blood cells, enabling appropriate treatment planning.

Test Code
606
Price
₹6,000
Sample Type
Whole Blood or Bone Marrow
Result Time
4 working days from sample receipt
Fasting Required
No
Method
FISH
Step 1

Sample Collection

Complete and submit the Chromosome & FISH analysis Requisition Form (Form 17) prior to sample collection.

Method: Venipuncture or Bone Marrow Aspiration

Step 2

Laboratory Analysis

Blood sample will be drawn via venipuncture; bone marrow sample will be collected via aspiration under local anesthesia.

Step 3

Report Delivery

Ensure the sample is stored at 18-22°C and shipped promptly. Do not freeze.

Timeline: 4 working days from sample receipt

Patient Instructions

1
Before the Test:Ensure all paperwork is complete and sample collection is scheduled.
2
During the Test:The sample is processed in the laboratory for FISH analysis.
3
After the Test:Results are compiled and sent via the chosen delivery method.

About This Test

Who Should Get This Test

To diagnose Acute Promyelocytic Leukemia (APL) by detecting the PML/RARA gene fusion in bone marrow or blood cells, enabling appropriate treatment planning.

How to Prepare

  • Collect 5 mL whole blood or 4 mL bone marrow
  • Use green top (sodium heparin) tubes
  • Maintain sample at room temperature (18-22°C)
  • Do not freeze the sample
  • Label the sample correctly with patient details

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"Detection of PML/RARA fusion is key for initiating all-transretinoic acid therapy in APL, leading to improved outcomes."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeWhole Blood or Bone Marrow
Sample Volume5 mL (3 mL min.) whole blood OR 4 mL (2 mL min.) Bone Marrow
ContainerGreen Top (Sodium Heparin) tubes
Collection MethodVenipuncture or Bone Marrow Aspiration

Sample Stability

Room Temperature
Refrigerator
Frozen
Sample Rejection Criteria:
  • Frozen samples
  • Samples in incorrect tubes (e.g., EDTA)
  • Insufficient volume or hemolyzed samples

Understanding Your Results

The test result indicates whether the PML/RARA gene fusion is present in the sample.
📊

Positive

Detection of PML/RARA fusion confirms Acute Promyelocytic Leukemia (APL). This finding guides treatment with all-transretinoic acid therapy.

📊

Negative

No PML/RARA fusion detected. APL is unlikely, but clinical correlation with other tests is recommended.

⚠️ When to Consult a Doctor:

Consult a hematologist or oncologist if you experience persistent unexplained bleeding, fatigue, fever, or other symptoms of leukemia.

Limitations

  • ⚠FISH may not detect cryptic or variant translocations
  • ⚠Requires specialized laboratory equipment and expertise

Risks & Considerations

  • ●Minor pain or bruising at the blood collection site
  • ●Rare risk of infection at the bone marrow aspiration site

Interfering Factors

  • ●Degraded or improperly collected sample
  • ●Low cellularity in bone marrow
  • ●Contamination during sample handling

Compare With Similar Tests

TestFISH - t(15;17) or LSI PML/RARA TestComplete Blood Count (CBC)Bone Marrow BiopsyFlow Cytometry
ComparisonFISH - t(15;17) or LSI PML/RARA TestCBC provides general blood cell counts but cannot detect specific gene fusions. FISH is confirmatory for APL.Biopsy assesses cellular morphology, while FISH identifies the exact genetic abnormality.Flow cytometry identifies cell surface markers; FISH detects chromosomal translocations.

Frequently Asked Questions

What is the FISH - t(15;17) or LSI PML/RARA test?
It is a molecular test using Fluorescence in situ hybridization to detect the PML/RARA gene fusion, which is indicative of Acute Promyelocytic Leukemia (APL).
Why is this test recommended?
This test is recommended to confirm diagnosis of APL, especially in patients with symptoms like bleeding disorders or unexplained fatigue, and to guide treatment decisions.
What are the common symptoms that may necessitate this test?
Symptoms include easy bruising, bleeding gums, petechiae (small red spots), fatigue, fever, and in severe cases, disseminated intravascular coagulation (DIC).
How is the sample collected for the test?
A blood sample (5 mL) or bone marrow sample (4 mL) is collected in sodium heparin tubes and shipped at room temperature.
What is the cost of the FISH t(15;17) PML/RARA test in India?
The test costs INR 6000 at DNA Labs India, which includes home sample collection in major cities.
How long does it take to get the test results?
Results are typically available within 4 working days from the date of sample collection.
What does a positive test result mean?
A positive result indicates the presence of PML/RARA gene fusion, confirming Acute Promyelocytic Leukemia (APL), which is treatable with specific therapies.
What does a negative test result mean?
A negative result means no PML/RARA fusion was detected, making APL unlikely, but other diagnostic tests may be needed.
Is home sample collection available for this test?
Yes, DNA Labs India offers free home sample collection for online bookings across numerous cities in India.
Do I need to fast before the test?
No, fasting is not required for the FISH t(15;17) PML/RARA test.
What is the turnaround time for the test?
The test has a turnaround time of 4 working days for report generation.
How accurate is the FISH test for detecting PML/RARA fusion?
FISH is a highly accurate and specific method for detecting the PML/RARA gene fusion, with sensitivity and specificity above 95% in experienced laboratories.
Can the FISH test detect all variants of the PML/RARA fusion?
While FISH is highly specific, it may not detect cryptic or variant translocations. In such cases, additional molecular tests like PCR may be recommended for confirmation.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

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