HLA - ABC (Class 1) Typing Test
Short Name: HLA Class I Typing
Also known as: HLA Class I Typing, HLA A B C Typing, MHC Class I Typing, Human Leukocyte Antigen Class I Test, HLA ABC Serology
HLA - ABC (Class 1) Typing Test test available at DNA Labs India for ₹12,051. Uses Reverse SSOP - Luminex on Whole Blood samples. Results in 10-12 days from sample collection. Free home collection in 300+ cities across India.
Overview
The primary purpose of the HLA - ABC (Class 1) Typing Test is to determine the HLA-A, HLA-B, and HLA-C allele profile of an individual. This information is critical for organ and tissue transplantation compatibility assessment, where matching HLA antigens between the donor and recipient significantly reduces the risk of graft rejection and improves transplant outcomes. The test is also used to assess disease susceptibility for autoimmune conditions, to support hematopoietic stem cell transplant (bone marrow) donor matching, and for forensic identification purposes. Identifying the HLA profile helps physicians make informed decisions about transplant eligibility and donor selection.
- Test Code
- 779
- CPT Code
- 81372
- ICD Code
- Z00.6
- Price
- ₹12,051
- Sample Type
- Whole Blood
- Result Time
- 10-12 days from sample collection
- Fasting Required
- No
- Method
- Reverse SSOP - Luminex
Sample Collection
A doctor's prescription on official letterhead with one photograph each of the recipient and donor, duly stamped, is mandatory. A signed Consent Form for HLA Typing for Solid Organ Transplant (Form 33) must accompany the prescription. No fasting is required. Inform the phlebotomist about any recent blood transfusions or stem cell transplants.
Method: Venipuncture
Laboratory Analysis
A trained phlebotomist will collect approximately 4 mL of whole blood via venipuncture into a Lavender Top (EDTA) tube or 6 mL into a Yellow Top (ACD) tube. The procedure typically takes less than 5 minutes.
Report Delivery
Apply gentle pressure on the venipuncture site with cotton wool for 3-5 minutes. Avoid strenuous activity with the arm used for collection. The blood sample will be shipped refrigerated to the laboratory. Do not freeze the sample.
Timeline: 10-12 days from sample collection
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of the HLA - ABC (Class 1) Typing Test is to determine the HLA-A, HLA-B, and HLA-C allele profile of an individual. This information is critical for organ and tissue transplantation compatibility assessment, where matching HLA antigens between the donor and recipient significantly reduces the risk of graft rejection and improves transplant outcomes. The test is also used to assess disease susceptibility for autoimmune conditions, to support hematopoietic stem cell transplant (bone marrow) donor matching, and for forensic identification purposes. Identifying the HLA profile helps physicians make informed decisions about transplant eligibility and donor selection.
How to Prepare
- Collect 4 mL (minimum 3 mL) whole blood in 1 Lavender Top (EDTA) tube OR 6 mL (minimum 3 mL) in 1 Yellow Top (ACD) tube
- Ship the sample refrigerated. DO NOT FREEZE the sample
- Attach the doctor's prescription on letterhead with stamped photographs of recipient and donor
- Include the signed Consent Form for HLA Typing for Solid Organ Transplant (Form 33)
- Label the sample correctly with patient name, date of birth, and sample collection date
- Ensure the sample tube is gently mixed 8-10 times after collection to prevent clotting
Doctor's Notes
"HLA ABC Class I typing is a cornerstone investigation in solid organ transplantation. A high-resolution match at HLA-A, HLA-B, and HLA-C loci between recipient and donor significantly improves graft survival and reduces the risk of acute and chronic rejection. This test should always be interpreted alongside crossmatch results and panel reactive antibody (PRA) levels for a comprehensive transplant workup. Early typing allows timely donor identification and better transplant outcomes."
Test Parameters & Specifications
Sample Stability
- Hemolyzed, clotted, or insufficient sample volume
- Missing doctor's prescription on letterhead with stamped photographs
- Missing or incomplete Consent Form for HLA Typing for Solid Organ Transplant (Form 33)
- Sample shipped frozen or at room temperature for extended periods
- Sample received more than 96 hours after collection
- Incorrect sample container used
Understanding Your Results
The donor and recipient share identical HLA-A, HLA-B, and HLA-C antigens. This is the ideal scenario for transplantation with the lowest risk of immune-mediated graft rejection.
The donor and recipient share most but not all HLA Class I antigens. Transplantation may still proceed but may require closer immunological monitoring and potentially more intensive immunosuppressive therapy.
Significant HLA mismatch increases the risk of acute and chronic graft rejection. Alternative donors should be considered if available. If no better match exists, enhanced immunosuppression protocols may be needed.
Certain HLA alleles are associated with increased susceptibility to specific autoimmune conditions. For example, HLA-B27 is strongly associated with ankylosing spondylitis. Results should be correlated with clinical findings and other laboratory data.
Consult your transplant physician, nephrologist, or hematologist to interpret HLA typing results in the context of your transplant workup. If disease-susceptibility HLA alleles are detected, consult a rheumatologist or immunologist for further evaluation. Always discuss results with your treating specialist before making any clinical decisions.
Limitations
- ⚠This test identifies Class I HLA antigens only (HLA-A, HLA-B, HLA-C); Class II typing (HLA-DR, HLA-DQ, HLA-DP) requires a separate test
- ⚠Molecular typing may report alleles at antigen-level or allele-level resolution depending on methodology
- ⚠Rare or novel alleles may not be identified by the probe set used and may require additional testing
- ⚠This test does not assess the functional status of the immune system
- ⚠Results should be interpreted alongside crossmatch and PRA testing for transplant workup
Risks & Considerations
- ●Minor bruising or discomfort at the venipuncture site
- ●Rare risk of fainting during blood draw
- ●Very rare risk of infection at the puncture site
- ●No significant long-term risks associated with the test itself
Interfering Factors
- ●Recent blood transfusion (within 3-6 months) may interfere with accurate HLA typing due to circulating donor DNA
- ●Bone marrow or stem cell transplant recipients may have mixed chimerism affecting results
- ●Sample contamination or improper handling can affect molecular assay accuracy
- ●Hemolyzed or clotted blood samples may yield unreliable results
- ●Improper storage temperature during sample transit can degrade DNA quality
Compare With Similar Tests
| Test | HLA - ABC (Class 1) Typing Test | HLA - DQ (Class 2) Typing Test | HLA - DR (Class 2) Typing Test | Crossmatch Test | Panel Reactive Antibody (PRA) Test |
|---|---|---|---|---|---|
| Comparison | HLA - ABC (Class 1) Typing Test | While HLA Class I typing covers HLA-A, B, C antigens expressed on all nucleated cells, HLA Class II DQ typing identifies HLA-DQ antigens primarily expressed on antigen-presenting cells. Both are needed for complete transplant compatibility assessment. | HLA-DR typing identifies Class II antigens that are crucial for transplant matching, particularly in kidney and bone marrow transplants. Combined with Class I typing, it provides a comprehensive HLA profile. | The crossmatch test determines whether the recipient has pre-formed antibodies against the specific donor's HLA antigens. It complements HLA typing by assessing the immunological compatibility in real-time before transplantation. | PRA testing measures the percentage of the population against which a recipient has pre-formed HLA antibodies. It indicates sensitization level and helps assess transplant difficulty. Unlike HLA typing which identifies the recipient's own antigens, PRA identifies antibodies against others' antigens. |
Frequently Asked Questions
What is the HLA - ABC (Class 1) Typing Test?
Why is the HLA - ABC (Class 1) Typing Test needed?
What is the cost of the HLA - ABC (Class 1) Typing Test at DNA Labs India?
What sample is required for the HLA - ABC (Class 1) Typing Test?
How long does it take to get the results of the HLA - ABC (Class 1) Typing Test?
Is fasting required before the HLA - ABC (Class 1) Typing Test?
What documents are required for the HLA - ABC (Class 1) Typing Test?
What are HLA Class I antigens and why are they important?
Who should get the HLA - ABC (Class 1) Typing Test?
Is the HLA - ABC (Class 1) Typing Test painful?
Can the HLA - ABC (Class 1) Typing Test diagnose autoimmune diseases?
What happens if my HLA does not match the donor's HLA?
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