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HLA - Panel Reactive IgG Antibodies (PRA) Class II Quantitative Test

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HLA - Panel Reactive IgG Antibodies (PRA) Class II Quantitative Test

Short Name: HLA PRA Class II Quantitative

Also known as: PRA Class II Quantitative, HLA Antibody Test

HLA - Panel Reactive IgG Antibodies (PRA) Class II Quantitative Test test available at DNA Labs India for ₹15,000. Uses Luminex X-Map technology on Serum samples. Results in 10-12 days. Free home collection in 300+ cities across India.

Immunology TestAdults🏠 Home Collection

Overview

To determine the level of pre-formed antibodies against HLA Class II antigens, which is critical for assessing transplant compatibility and predicting the risk of rejection.

Test Code
798
Price
₹15,000
Sample Type
Serum
Result Time
10-12 days
Fasting Required
No
Method
Luminex X-Map technology
Step 1

Sample Collection

Collect specimen preferably 48 hours after dialysis. Provide brief clinical history.

Method: Venipuncture

Step 2

Laboratory Analysis

Standard venipuncture procedure.

Step 3

Report Delivery

Apply pressure to the puncture site to stop bleeding.

Timeline: 10-12 days

Patient Instructions

1
Before the Test:No special preparation, but inform doctor about medications and medical history.
2
During the Test:A blood sample will be drawn from your arm.
3
After the Test:You may resume normal activities. Apply bandage if needed.

About This Test

Who Should Get This Test

To determine the level of pre-formed antibodies against HLA Class II antigens, which is critical for assessing transplant compatibility and predicting the risk of rejection.

How to Prepare

  • Fasting not required, but collect 48 hours after dialysis if applicable
  • Provide clinical history including transfusion and transplant history
  • Use SST tube and refrigerate sample

Doctor's Notes

"High PRA levels indicate a higher risk of organ rejection and require careful donor matching for successful transplantation."

Test Parameters & Specifications

Sample TypeSerum
Sample Volume3 mL (1.5 mL minimum)
ContainerSST (Serum Separator Tube)
Collection MethodVenipuncture

Sample Stability

Room Temperature: 2 hours
Refrigerator: 1 week
Frozen: 4 weeks
Sample Rejection Criteria:
  • Hemolyzed samples
  • Insufficient volume
  • Incorrect sample type

Understanding Your Results

The PRA Class II percentage indicates the proportion of the population that the patient's antibodies would react with. Higher values suggest greater sensitization and increased risk of rejection.
📊

Low sensitization; lower risk of rejection

Range: 0-10%

📊

Moderate sensitization; requires careful donor matching

Range: 11-80%

📊

High sensitization; significant barrier to transplantation

Range: >80%

⚠️ When to Consult a Doctor:

If PRA levels are high or if there are signs of rejection post-transplant.

Limitations

  • Does not specify specific antibodies
  • May not detect all HLA antibodies
  • Results can be affected by assay sensitivity

Risks & Considerations

  • Minor bruising or pain at puncture site
  • Rarely, infection or dizziness

Interfering Factors

  • Hemolyzed samples
  • Recent blood transfusions
  • Certain medications affecting immune response

Compare With Similar Tests

TestHLA - Panel Reactive IgG Antibodies (PRA) Class II Quantitative Test
ComparisonHLA - Panel Reactive IgG Antibodies (PRA) Class II Quantitative Test

Frequently Asked Questions

What is HLA - Panel Reactive IgG Antibodies (PRA) Class II Quantitative Test?
It is a blood test that measures the level of antibodies against HLA Class II antigens to assess sensitization for organ transplantation.
Why is this test performed?
To determine compatibility for organ transplantation and evaluate the risk of antibody-mediated rejection.
How is the test performed?
A blood sample is drawn from the arm and analyzed using Luminex X-Map technology to measure antibody levels.
What is the cost of the test in India?
The cost is INR 15000, with free home sample collection available across India.
What does a high PRA level mean?
A high PRA level indicates greater sensitization, which increases the risk of organ rejection and may require careful donor selection.
Is fasting required for this test?
Fasting is not required, but it is preferable to collect the specimen 48 hours after dialysis if applicable.
How long does it take to get the results?
Results are typically available within 10-12 days.
What are the risks of the test?
Risks are minimal and may include minor bruising or pain at the puncture site.
Who should undergo this test?
Patients awaiting organ transplantation, especially those with a history of transfusions or previous transplants.
How is this test different from HLA typing?
HLA typing identifies specific HLA antigens, while this test measures the overall level of antibodies against HLA antigens.
Can this test be done at home?
Yes, DNA Labs India offers free home sample collection for this test across numerous cities in India.
What should I do if my PRA levels are high?
Consult your healthcare provider for further evaluation and to discuss potential donor matching strategies.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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