PML RARA t(1517)(q22q12) Gene Rearrangement Qualitative PCR Test
Short Name: PML-RARA PCR
Also known as: PML-RARA translocation test, t(15;17) PCR, PML/RARA fusion gene test
PML RARA t(1517)(q22q12) Gene Rearrangement Qualitative PCR Test test available at DNA Labs India for ₹7,500. Uses Real Time PCR on Whole Blood samples. Results in Samples are accepted on Monday, Wednesday, and Friday by 9 am. Reports are issued on the following Tuesday, Thursday, and Saturday respectively (approximately 24 hours after acceptance).. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr SULOCHANA HEMCHANDRA HOLLA
Consultant Medical Geneticist · Reg: 8532
Last reviewed: September 7, 2026
Overview
To detect the PML-RARA gene rearrangement in patients with suspected acute promyelocytic leukemia (APL), confirm the diagnosis, and guide treatment decisions with all-trans retinoic acid (ATRA)-based therapy.
- Test Code
- 3558
- Price
- ₹7,500
- Sample Type
- Whole Blood
- Result Time
- Samples are accepted on Monday, Wednesday, and Friday by 9 am. Reports are issued on the following Tuesday, Thursday, and Saturday respectively (approximately 24 hours after acceptance).
- Fasting Required
- No
- Method
- Real Time PCR
Sample Collection
No fasting is required. A duly filled Genomics Clinical Information Requisition Form (Form 20) is mandatory. Inform your doctor about any current medications or recent blood transfusion.
Method: Venipuncture
Laboratory Analysis
A blood sample will be collected from a vein in the arm using a sterile needle. The procedure takes only a few minutes.
Report Delivery
You may immediately resume daily activities. Keep the pressure bandage on for a few minutes and avoid heavy lifting for an hour.
Timeline: Samples are accepted on Monday, Wednesday, and Friday by 9 am. Reports are issued on the following Tuesday, Thursday, and Saturday respectively (approximately 24 hours after acceptance).
Patient Instructions
About This Test
Who Should Get This Test
To detect the PML-RARA gene rearrangement in patients with suspected acute promyelocytic leukemia (APL), confirm the diagnosis, and guide treatment decisions with all-trans retinoic acid (ATRA)-based therapy.
How to Prepare
- Ensure the Genomics Clinical Information Requisition Form (Form 20) is completely filled and signed.
- Collect blood in two lavender top (EDTA) tubes with a minimum volume of 3 mL each (total 5 mL preferred).
- Label the tubes with patient identifiers and the date/time of collection.
- Ship the sample refrigerated (2-8°C) using a cold pack. Do not freeze.
- Transport the sample to the laboratory within 72 hours of collection.
Doctor's Notes
Reviewed by Dr SULOCHANA HEMCHANDRA HOLLA — MBBS, MD (Medical Genetics) · Reg. No. 8532
"Early detection of PML-RARA fusion via PCR is critical—APL is a medical emergency where timely ATRA therapy can change the course of the disease."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Clotted specimen
- Hemolyzed specimen
- Frozen specimen
- Incorrect tube type
- Improper labeling
- Missing requisition form
- Sample received after 72 hours
Understanding Your Results
Negative
No PML-RARA fusion detected. APL is less likely, though clinical correlation is needed.
Positive
PML-RARA fusion is detected, supporting a diagnosis of APL. ATRA-based therapy is indicated.
If you have symptoms suggestive of APL or if the test result is positive, seek urgent evaluation by an oncologist. Even a negative result does not rule out other blood conditions; follow your doctor's advice.
Limitations
- ⚠Qualitative test does not quantify fusion transcript levels; for monitoring residual disease, quantitative PCR may be required.
- ⚠Breakpoint variants outside the commonly tested regions may lead to false negative results.
- ⚠This test does not detect other gene rearrangements associated with APL.
- ⚠Results should be interpreted in conjunction with clinical findings and other laboratory tests.
Risks & Considerations
- ●Minimal risk of slight bruising or bleeding at the puncture site
- ●Rare risk of infection at the needle insertion site
Interfering Factors
- ●Degraded RNA due to delayed sample processing
- ●Sample collected in EDTA tube stored for more than 72 hours
- ●Clotted or insufficient sample
- ●Presence of PCR inhibitors
Compare With Similar Tests
| Test | PML RARA t(1517)(q22q12) Gene Rearrangement Qualitative PCR Test | Cytogenetic Analysis (Karyotyping) | Fluorescence In Situ Hybridization (FISH) |
|---|---|---|---|
| Comparison | PML RARA t(1517)(q22q12) Gene Rearrangement Qualitative PCR Test |
Frequently Asked Questions
What is the PML RARA t(15;17) qualitative PCR test?
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We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.
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