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PML RARA t(1517)(q22q12) Gene Rearrangement Quantitative PCR Test

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PML RARA t(1517)(q22q12) Gene Rearrangement Quantitative PCR Test

Short Name: PML RARA PCR

Also known as: PML-RARA t(15;17) Quantitative PCR, APL Fusion Gene Test, t(15;17) Gene Rearrangement PCR

PML RARA t(1517)(q22q12) Gene Rearrangement Quantitative PCR Test test available at DNA Labs India for ₹7,500. Uses Real Time PCR on Whole blood samples. Results in Reports are issued on Tuesday, Thursday, and Saturday for samples accepted on Monday, Wednesday, and Friday before 9 am.. Free home collection in 300+ cities across India.

Real Time PCR🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr SULOCHANA HEMCHANDRA HOLLA

Dr SULOCHANA HEMCHANDRA HOLLA

Consultant Medical Geneticist · Reg: 8532

Last reviewed: September 7, 2026

Overview

To detect and quantify PML RARA fusion transcripts in peripheral blood for diagnosis and monitoring of Acute Promyelocytic Leukemia (APL).

Test Code
3562
Price
₹7,500
Sample Type
Whole blood
Result Time
Reports are issued on Tuesday, Thursday, and Saturday for samples accepted on Monday, Wednesday, and Friday before 9 am.
Fasting Required
No
Method
Real Time PCR
Step 1

Sample Collection

No fasting required. Ensure the Genomics Clinical Information Requisition Form (Form 20) is completed and signed. Inform the lab about any recent blood transfusions or treatment.

Method: Venipuncture

Step 2

Laboratory Analysis

Whole blood is collected by venipuncture.

Step 3

Report Delivery

Refrigerate the sample at 2-8°C. Do not freeze. Transport the sample to the laboratory within 72 hours.

Timeline: Reports are issued on Tuesday, Thursday, and Saturday for samples accepted on Monday, Wednesday, and Friday before 9 am.

Patient Instructions

1
Before the Test:Complete the requisition form; inform clinician of all medications; no special preparation required.
2
During the Test:A technician will collect blood samples from your arm.
3
After the Test:You may resume normal activities immediately.

About This Test

Who Should Get This Test

To detect and quantify PML RARA fusion transcripts in peripheral blood for diagnosis and monitoring of Acute Promyelocytic Leukemia (APL).

How to Prepare

  • Fill 2 lavender top (EDTA) tubes with 5 mL and 3 mL minimum blood respectively.
  • Label each tube with patient name and unique ID.
  • Store at 2-8°C after collection.

Doctor's Notes

Reviewed by — MBBS, MD (Medical Genetics) · Reg. No. 8532

"Early detection of PML-RARA is key to successful treatment of APL; this test enables precise molecular monitoring."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeWhole blood
Sample Volume5 mL (3 mL min.)
Container2 Lavender Top (EDTA) tubes
Collection MethodVenipuncture

Sample Stability

Room temperature (20-25°C): 6 hours
Refrigerated (2-8°C): 72 hours
Frozen (≤-20°C): Not acceptable
Sample Rejection Criteria:
  • Sample received after 6 hours at room temperature
  • Frozen or hemolyzed sample
  • Sample in a tube other than EDTA
  • Missing/incomplete requisition form

Understanding Your Results

The PML RARA quantitative PCR test is interpreted based on the presence and level of PML RARA fusion transcripts normalized to an internal control gene (ABL).
Negative: No PML RARA fusion transcript detected. This suggests absence of t(15;17) or a complete molecular remission.
Positive: PML RARA transcript detected. Supports the diagnosis of APL and requires urgent clinical action.
Rising transcript levels (from a previously undetectable level) indicate molecular relapse and need for further evaluation.
⚠️ When to Consult a Doctor:

Seek immediate advice from an oncologist if you experience easy bruising, bleeding, persistent fever, pallor, shortness of breath, or unexplained weight loss.

Limitations

  • ⚠Test may not detect rare breakpoint variants
  • ⚠Qualitative result does not provide the exact translocation breakpoint
  • ⚠Interpretation requires correlation with clinical and cytogenetic findings

Risks & Considerations

  • ●Pain or discomfort at the venipuncture site
  • ●Minor bruising
  • ●Rarely, bleeding or infection

Interfering Factors

  • ●RNA degradation due to delayed sample processing or freeze-thaw
  • ●Heparin contamination from heparinized samples
  • ●PCR inhibitors from hemolysis or high bilirubin
  • ●Improper requisition form

Compare With Similar Tests

TestPML RARA t(1517)(q22q12) Gene Rearrangement Quantitative PCR Test
ComparisonPML RARA t(1517)(q22q12) Gene Rearrangement Quantitative PCR Test

Frequently Asked Questions

What is a PML RARA t(15;17)(q22;q12) gene rearrangement?
It is a genetic abnormality in which a fusion of the PML gene on chromosome 15 and RARA gene on chromosome 17 occurs. This is commonly found in acute promyelocytic leukemia.
Why is this quantitative PCR test ordered?
It is used to diagnose APL, monitor minimal residual disease, and assess response to treatment.
What is the cost of the test at DNA Labs India?
The test costs Rs 7500 at DNA Labs India, with free home sample collection for online bookings.
What sample is required?
5 mL (3 mL minimum) of whole blood is collected in 2 lavender top (EDTA) tubes.
Is fasting required before the test?
No, fasting is not required.
How long will it take to get results?
Reports are delivered on Tuesday, Thursday, and Saturday for samples accepted on Monday, Wednesday, and Friday by 9 am.
What does a negative result mean?
A negative result means the PML RARA fusion transcript was not detected, which may indicate absence of the translocation or a favorable response to treatment.
What does a positive result mean?
A positive result indicates the presence of PML RARA fusion transcript, which supports a diagnosis of APL and requires immediate clinical correlation.
How is the test performed?
The test uses real-time reverse transcription PCR (RT-qPCR) to detect PML RARA fusion transcripts in the RNA obtained from white blood cells in whole blood.
Does DNA Labs India offer home sample collection for this test?
Yes, free home sample collection is available for online bookings in many cities across India.
In which cities is the test available?
The test is available in Mumbai, Delhi, Bangalore, Hyderabad, Ahmedabad, Chennai, Kolkata, and many other locations. Please contact support for a full list of cities.
Is a doctor's prescription mandatory?
The test is ideally requested by an oncologist, but patients can book with a duly filled clinical requisition form.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

LIMS Integration

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Your medical data is protected under Indian law.

✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

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