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DNA Labs India

Pregnenolone Test

DNA Labs India | ISO 9001:2015 Certified

Pregnenolone Test

Short Name: PGS

Also known as: Preimplantation Genetic Testing for Aneuploidy (PGT-A), Comprehensive Chromosome Screening (CCS), Embryo Genetic Screening, IVF Genetic Screening, Embryo Aneuploidy Testing

Pregnenolone Test test available at DNA Labs India for ₹16,380. Uses Next Generation Sequencing (NGS), Comprehensive Chromosome Screening on Embryo Cells (Biopsied Trophectoderm or Blastomere) samples. Results in Sample received by Tuesday 9:00 AM — Report delivered by the following Tuesday (approximately 7–10 working days).. Free home collection in 300+ cities across India.

Genetic ScreeningBoth (Embryo)Reproductive Age Adults🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr SULOCHANA HEMCHANDRA HOLLA

Dr SULOCHANA HEMCHANDRA HOLLA

Consultant Medical Geneticist · Reg: 8532

Last reviewed: September 7, 2026

Overview

The primary purpose of Preimplantation Genetic Screening (PGS) is to evaluate embryos created through IVF for chromosomal abnormalities before they are transferred to the uterus. By identifying euploid (chromosomally normal) embryos, PGS aims to improve implantation success rates, reduce the risk of miscarriage, decrease the chance of conceiving a child with a chromosomal disorder, and minimize the need for multiple IVF cycles. PGS empowers couples and their fertility specialists to make evidence-based decisions about embryo selection, ultimately increasing the likelihood of a healthy live birth.

Test Code
1398
CPT Code
81228
ICD Code
Z31.49
Price
₹16,380
Sample Type
Embryo Cells (Biopsied Trophectoderm or Blastomere)
Result Time
Sample received by Tuesday 9:00 AM — Report delivered by the following Tuesday (approximately 7–10 working days).
Fasting Required
No
Method
Next Generation Sequencing (NGS), Comprehensive Chromosome Screening
Step 1

Sample Collection

A duly filled Preimplantation Genetic Screening Consent form (Form 23) is mandatory before sample submission. The fertility clinic must coordinate with DNA Labs India to schedule sample collection and dispatch. Embryo biopsy is performed by a trained embryologist during the IVF cycle, typically at the blastocyst stage (day 5 or 6). Couples are advised to undergo genetic counseling before the procedure to understand the implications, benefits, and limitations of PGS.

Method: Embryo biopsy during IVF cycle (performed by embryologist)

Step 2

Laboratory Analysis

The embryologist performs a trophectoderm biopsy, removing 5–10 cells from the outer layer of the blastocyst-stage embryo. Biopsied cells are placed into a unique transport tube available from LPL. For each embryo being tested, a separate tube and line submission is required. The embryos may be vitrified (frozen) while results are awaited.

Step 3

Report Delivery

After biopsy, the embryo is typically vitrified and stored until results are available. Biopsied samples are shipped frozen in special transport containers provided by LPL. PGS results are generally available within 7–10 working days. Genetic counseling is provided upon delivery of results to help interpret findings and plan next steps for embryo transfer.

Timeline: Sample received by Tuesday 9:00 AM — Report delivered by the following Tuesday (approximately 7–10 working days).

Patient Instructions

1
Before the Test:Prior to PGS, couples should undergo a comprehensive genetic counseling session to understand the benefits, risks, and limitations of the test. A duly filled Preimplantation Genetic Screening Consent form (Form 23) must be completed and submitted. The IVF cycle must be underway, and embryo development to the blastocyst stage (day 5–6) is required for biopsy. No fasting is required from the patient; however, the embryo biopsy must be scheduled in coordination with the IVF clinic and DNA Labs India.
2
During the Test:PGS involves a minimally invasive embryo biopsy performed by a skilled embryologist in the IVF laboratory. Using micromanipulation techniques under a microscope, 5–10 cells are carefully removed from the trophectoderm (outer cell layer) of a blastocyst-stage embryo. The biopsied cells are placed in a labeled transport tube, and the embryo is vitrified (frozen) pending results. The procedure does not typically damage the embryo when performed by experienced professionals.
3
After the Test:After biopsy, the embryo is stored frozen. The biopsied sample is shipped frozen to DNA Labs India's laboratory for analysis via Next Generation Sequencing. Results are typically available within 7–10 working days. Upon result availability, a genetic counseling session is conducted to explain findings and assist in planning the embryo transfer cycle. Euploid embryos are prioritized for frozen embryo transfer (FET).

About This Test

Who Should Get This Test

The primary purpose of Preimplantation Genetic Screening (PGS) is to evaluate embryos created through IVF for chromosomal abnormalities before they are transferred to the uterus. By identifying euploid (chromosomally normal) embryos, PGS aims to improve implantation success rates, reduce the risk of miscarriage, decrease the chance of conceiving a child with a chromosomal disorder, and minimize the need for multiple IVF cycles. PGS empowers couples and their fertility specialists to make evidence-based decisions about embryo selection, ultimately increasing the likelihood of a healthy live birth.

How to Prepare

  • Submit biopsied embryo cells in a unique tube available from LPL
  • In case of multiple embryos being tested, submit a separate line and tube for each embryo source
  • Ship frozen in special transport containers available from LPL
  • A duly filled Preimplantation Genetic Screening Consent form (Form 23) is mandatory
  • Sample to be collected and dispatched with prior appointment only
  • Ensure proper labeling with patient identification and embryo number on each tube
  • Maintain cold chain during transport to preserve sample integrity

Doctor's Notes

Reviewed by — MBBS, MD (Medical Genetics) · Reg. No. 8532

"Preimplantation Genetic Screening is a critical advancement in assisted reproductive technology. By screening embryos for chromosomal aneuploidy prior to uterine transfer, PGS significantly improves implantation rates and reduces the likelihood of miscarriage. I recommend PGS for couples with a history of recurrent pregnancy loss, advanced maternal age, or previous failed IVF cycles. It empowers patients to make informed decisions about embryo selection and increases the probability of a healthy live birth. Always discuss with your reproductive medicine specialist whether PGS is appropriate for your individual clinical scenario."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeEmbryo Cells (Biopsied Trophectoderm or Blastomere)
Sample Volume5–10 cells per embryo
ContainerUnique transport tube available from LPL
Collection MethodEmbryo biopsy during IVF cycle (performed by embryologist)

Sample Stability

Frozen (-196°C in liquid nitrogen)
Room Temperature
Refrigerated (2–8°C)
Sample Rejection Criteria:
  • Missing or incomplete Preimplantation Genetic Screening Consent form (Form 23)
  • Sample not shipped in designated LPL transport containers
  • Sample received at room temperature without frozen cold chain
  • Insufficient number of biopsied cells for analysis
  • Mislabeled or unlabeled sample tubes
  • Sample without prior appointment confirmation

Understanding Your Results

PGS results classify each tested embryo as euploid (chromosomally normal), aneuploid (chromosomally abnormal), or mosaic (containing a mixture of normal and abnormal cells). Your reproductive medicine specialist and genetic counselor will discuss what the results mean for your IVF treatment plan. Only euploid embryos are recommended for uterine transfer to maximize the chance of a successful, healthy pregnancy.
📊

Euploid (Normal)

The embryo has the correct number of chromosomes (46,XX or 46,XY). This embryo is recommended for transfer as it has the highest probability of resulting in a healthy pregnancy.

Clinical action: Proceed with frozen embryo transfer (FET) cycle as guided by your fertility specialist.

📊

Aneuploid (Abnormal)

The embryo has an abnormal number of chromosomes (e.g., trisomy or monosomy). Transferring an aneuploid embryo carries a high risk of implantation failure, miscarriage, or a child with a chromosomal disorder.

Clinical action: This embryo is generally not recommended for transfer. Discuss options with your fertility specialist.

📊

Mosaic

The embryo contains a mixture of chromosomally normal and abnormal cells. The clinical outcome depends on the type and level of mosaicism detected.

Clinical action: Transfer of mosaic embryos may be considered in select cases when no euploid embryos are available, with thorough genetic counseling and informed consent.

📊

No Result / Inconclusive

The analysis could not produce a clear result due to insufficient DNA, sample degradation, or technical issues.

Clinical action: Re-biopsy of the embryo (if still available) or re-testing may be recommended. Discuss with your fertility team.

⚠️ When to Consult a Doctor:

Consult your reproductive medicine specialist or genetic counselor if you are planning IVF and have a history of recurrent miscarriages, are of advanced maternal age, have a family history of chromosomal abnormalities or genetic disorders, or have experienced repeated IVF failures. A genetic counselor should also be consulted to discuss PGS results before making decisions about embryo transfer.

Limitations

  • ⚠PGS screens for chromosomal number abnormalities but does not detect all single gene disorders unless specifically ordered as PGT-M
  • ⚠Mosaicism within an embryo may result in discordance between the biopsy result and the actual chromosomal status of the embryo
  • ⚠A euploid result does not guarantee a successful pregnancy or a healthy live birth
  • ⚠The test cannot detect structural birth defects, epigenetic disorders, or non-genetic causes of pregnancy loss
  • ⚠Biopsy of the embryo carries a small risk of damage to the embryo, though modern techniques minimize this risk

Risks & Considerations

  • ●Small risk of embryo damage during biopsy, though modern trophectoderm biopsy techniques have significantly reduced this risk
  • ●False positives or false negatives may occur due to mosaicism or technical limitations
  • ●Emotional stress associated with receiving abnormal results or inconclusive findings
  • ●The biopsy does not alter the embryo's genetic makeup but removes a small number of cells
  • ●A normal PGS result does not eliminate all risks of genetic disease or pregnancy complications

Interfering Factors

  • ●Embryo quality: Poor-quality embryos may not yield sufficient cells for accurate analysis
  • ●Mosaicism: Mixed populations of normal and abnormal cells within an embryo can lead to ambiguous results
  • ●Sample handling: Improper storage, shipping, or transport conditions may affect DNA integrity
  • ●Contamination: External DNA contamination during biopsy or processing may compromise results
  • ●Technical limitations: Very low-level mosaicism may not be detected by current NGS methods

Compare With Similar Tests

TestPregnenolone TestPreimplantation Genetic Diagnosis (PGD/PGT-M)Non-Invasive Prenatal Testing (NIPT)AmniocentesisChorionic Villus Sampling (CVS)
ComparisonPregnenolone TestPGD tests for specific known single-gene disorders (e.g., cystic fibrosis, sickle cell disease) in embryos, while PGS screens broadly for chromosomal number abnormalities.NIPT screens for chromosomal abnormalities in a fetus during pregnancy using maternal blood, whereas PGS screens embryos before implantation during IVF.Amniocentesis is a prenatal diagnostic test performed during pregnancy (15–20 weeks) to detect chromosomal and genetic abnormalities. PGS is performed before pregnancy, during the IVF process.CVS is a first-trimester prenatal diagnostic test. Unlike PGS, it is performed after implantation and pregnancy has been established, typically around 10–13 weeks of gestation.

Frequently Asked Questions

What is Preimplantation Genetic Screening (PGS)?
Preimplantation Genetic Screening (PGS), also known as PGT-A (Preimplantation Genetic Testing for Aneuploidy), is a genetic test performed on embryos created through IVF. It screens all 23 pairs of chromosomes to identify chromosomal abnormalities before the embryo is transferred to the uterus. This helps select the most viable embryo for implantation, increasing the chances of a healthy pregnancy.
Who should consider PGS testing?
PGS is recommended for couples undergoing IVF who have a history of recurrent miscarriages, advanced maternal age (35 years or older), repeated failed IVF cycles, severe male factor infertility, or a family history of chromosomal abnormalities. Your fertility specialist can help determine whether PGS is appropriate for your situation.
How is the PGS test performed?
During the IVF process, when embryos reach the blastocyst stage (day 5 or 6), an embryologist performs a trophectoderm biopsy, removing 5–10 cells from the outer layer of the embryo. These cells are sent to the DNA Labs India laboratory, where they are analyzed using Next Generation Sequencing (NGS) to check for chromosomal abnormalities.
What is the cost of the PGS test at DNA Labs India?
The PGS test at DNA Labs India costs INR 16380. This price includes the genetic analysis using Next Generation Sequencing and a genetic counseling session to help you understand your results and make informed decisions about your IVF treatment.
Does PGS guarantee a successful pregnancy?
No, PGS does not guarantee a successful pregnancy. While selecting a euploid (chromosomally normal) embryo significantly improves implantation rates and reduces the risk of miscarriage, many other factors—including uterine receptivity, hormonal balance, and overall health—also influence pregnancy outcomes.
Is the embryo biopsy procedure safe? Does it harm the embryo?
Modern trophectoderm biopsy techniques are considered safe and are performed by highly skilled embryologists. The biopsy removes a small number of cells from the outer layer of the blastocyst, which does not contribute to the fetus itself. While there is a very small risk of embryo damage, the overall risk is minimal when performed by experienced professionals.
How long does it take to get PGS results?
PGS results at DNA Labs India are typically available within 7–10 working days from the date the sample is received at the laboratory. Your fertility clinic and genetic counselor will discuss the results with you once they are available.
Can PGS detect all genetic disorders?
PGS screens for chromosomal number abnormalities (aneuploidies) such as Down syndrome, Turner syndrome, and Klinefelter syndrome. It does not detect single-gene disorders (like cystic fibrosis or sickle cell disease) unless a separate test called PGD (Preimplantation Genetic Diagnosis / PGT-M) is specifically ordered. Your genetic counselor can advise on the appropriate testing for your needs.
What happens if all my embryos are found to be aneuploid?
If all tested embryos are aneuploid, your fertility specialist will discuss your options, which may include undergoing another IVF cycle to create new embryos. In some cases, mosaic embryos (with a mix of normal and abnormal cells) may be considered for transfer after thorough genetic counseling and informed consent, depending on the type and level of mosaicism.
Do I need genetic counseling before PGS testing?
Yes, genetic counseling before PGS testing is strongly recommended and is included as part of the PGS service at DNA Labs India. A genetic counselor will explain the benefits, limitations, and potential outcomes of the test, ensuring you can make fully informed decisions about your embryos and treatment plan.
Is PGS the same as PGD (Preimplantation Genetic Diagnosis)?
No. PGS (PGT-A) screens embryos broadly for chromosomal number abnormalities without targeting a specific genetic condition. PGD (PGT-M) tests embryos for a specific known single-gene disorder or chromosomal structural rearrangement that runs in the family. Your fertility specialist will recommend the appropriate test based on your medical and family history.
How do I submit my embryo sample for PGS at DNA Labs India?
The embryo biopsy is performed at your IVF clinic by a trained embryologist. The biopsied cells must be submitted in a unique LPL transport tube, shipped frozen in special transport containers provided by LPL. A completed Preimplantation Genetic Screening Consent form (Form 23) is mandatory. For multiple embryos, a separate tube and submission line is required for each embryo. Sample collection and dispatch must be arranged by prior appointment with DNA Labs India.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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