Vitamin D 25 - Hydroxy Test
Short Name: Vitamin D 25-Hydroxy Test
Also known as: 25-Hydroxy Vitamin D Test, Vitamin D Total, 25(OH)D Test, Calcidiol Test
Vitamin D 25 - Hydroxy Test test available at DNA Labs India for ₹1,814. Uses Chemiluminescence on Serum samples. Results in Reports are generally available on the same day if the sample is collected in the morning; otherwise by the next working day. Exact time may vary by city and lab location.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Ramarao Paidisetty
Consultant Biochemist · Reg: 21504
Last reviewed: September 7, 2026
Overview
The main purpose of measuring 25-hydroxy vitamin D is to assess an individual's vitamin D status. It is widely used to diagnose deficiency, identify causes of bone disease, and monitor the effectiveness of vitamin D replacement therapy.
- Test Code
- 3740
- CPT Code
- 82306
- ICD Code
- E55.9
- Price
- ₹1,814
- Sample Type
- Serum
- Result Time
- Reports are generally available on the same day if the sample is collected in the morning; otherwise by the next working day. Exact time may vary by city and lab location.
- Fasting Required
- No
- Method
- Chemiluminescence
Sample Collection
No special preparation is required. Continue your usual diet and hydration. Tell your doctor about any supplements, especially vitamin D, calcium, and biotin.
Method: Venipuncture
Laboratory Analysis
A trained phlebotomist will draw a blood sample from a vein in your arm. The procedure takes about 5 minutes and is generally well tolerated.
Report Delivery
You can resume normal activities immediately. Mild bruising or soreness at the puncture site may occur and usually resolves quickly.
Timeline: Reports are generally available on the same day if the sample is collected in the morning; otherwise by the next working day. Exact time may vary by city and lab location.
Patient Instructions
About This Test
Who Should Get This Test
The main purpose of measuring 25-hydroxy vitamin D is to assess an individual's vitamin D status. It is widely used to diagnose deficiency, identify causes of bone disease, and monitor the effectiveness of vitamin D replacement therapy.
How to Prepare
- No fasting required
- Inform your doctor if you are taking biotin supplements
- Wear clothing with easy access to your arm
- Keep the sample refrigerated if testing is delayed
Doctor's Notes
Reviewed by Dr Ramarao Paidisetty — MBBS, MD (Biochemistry) · Reg. No. 21504
"In clinical practice, I often see patients with non-specific fatigue, bone pain, and muscle weakness. A 25-hydroxy vitamin D test helps identify whether vitamin D deficiency is contributing to these symptoms. I recommend interpreting the result together with serum calcium, phosphorus, and PTH, especially before starting high-dose supplementation."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed, grossly lipemic, or icteric samples
- Unlabeled or mislabeled sample
- Sample stored at room temperature for more than 8 hours
- Sample received without proper cold transport if delay is expected
Understanding Your Results
Indicates vitamin D deficiency. Supplementation may be required after physician assessment.
Suboptimal vitamin D stores; address risk factors and consider supplementation.
Adequate vitamin D status for bone and general health in most individuals.
Elevated level may indicate hypervitaminosis D; seek medical evaluation.
Consult your doctor if the test report is abnormal, or if you have symptoms like persistent fatigue, bone pain, muscle weakness, or mood changes.
Limitations
- ⚠This test measures total 25(OH)D (D2 + D3), not free or bioavailable vitamin D
- ⚠It does not directly measure the active hormone calcitriol
- ⚠Results must be interpreted alongside clinical findings and other laboratory values
Risks & Considerations
- ●Mild pain or bruising at the needle site
- ●Rare localized infection
- ●Dizziness or lightheadedness during blood draw
Interfering Factors
- ●Biotin supplementation may interfere with immunoassay methods
- ●Very high calcium or phosphate levels may complicate interpretation
- ●Hemolyzed or lipemic samples may affect accuracy
- ●Season, sun exposure, and recent diet affect vitamin D levels
Compare With Similar Tests
| Test | Vitamin D 25 - Hydroxy Test | ||
|---|---|---|---|
| Comparison | Vitamin D 25 - Hydroxy Test |
Frequently Asked Questions
What is the Vitamin D 25-Hydroxy test?
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