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DNA Labs India

Vitamin D Screen Test

DNA Labs India | ISO 9001:2015 Certified

Vitamin D Screen Test

Short Name: Vitamin D Screen

Also known as: 25-Hydroxy Vitamin D Test, 25(OH)D Test, Calcidiol Test, Vitamin D Total Test

Vitamin D Screen Test test available at DNA Labs India for ₹3,000. Uses CLIA, Spectrophotometry on Serum samples. Results in Reports are typically available within 24 hours after the sample reaches the laboratory.. Free home collection in 300+ cities across India.

Hormone PanelAll Ages🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr Ramarao Paidisetty

Dr Ramarao Paidisetty

Consultant Biochemist · Reg: 21504

Last reviewed: September 7, 2026

Overview

To screen and diagnose Vitamin D deficiency or insufficiency, assess calcium metabolism, and guide supplementation or further endocrine evaluation.

Test Code
3739
CPT Code
82306
ICD Code
E55.9
Price
₹3,000
Sample Type
Serum
Result Time
Reports are typically available within 24 hours after the sample reaches the laboratory.
Fasting Required
No
Method
CLIA, Spectrophotometry
Step 1

Sample Collection

No special preparation required. Continue your regular diet and medications unless advised by your doctor. Please inform your doctor about any supplements you are taking, especially biotin or vitamin D supplements.

Method: Venipuncture

Step 2

Laboratory Analysis

A blood sample will be drawn from a vein in your arm. The procedure takes about 5 minutes. You may feel a brief pinch when the needle is inserted.

Step 3

Report Delivery

You may resume normal activities immediately. Apply pressure to the collection site if needed. Stay hydrated and eat normally.

Timeline: Reports are typically available within 24 hours after the sample reaches the laboratory.

Patient Instructions

1
Before the Test:No special preparation required. Continue your regular diet and medications unless advised by your doctor. Inform your doctor about any supplements you are taking, especially biotin or vitamin D supplements.
2
During the Test:A blood sample will be drawn from a vein in your arm. The procedure takes about 5 minutes. You may feel a brief pinch when the needle is inserted.
3
After the Test:You may resume normal activities immediately. Apply pressure to the collection site if needed. Stay hydrated and eat normally.

About This Test

Who Should Get This Test

To screen and diagnose Vitamin D deficiency or insufficiency, assess calcium metabolism, and guide supplementation or further endocrine evaluation.

How to Prepare

  • No fasting required.
  • Inform lab about biotin supplements.
  • Sample should be collected in SST tube.
  • Keep sample refrigerated if not sent immediately.

Doctor's Notes

Reviewed by — MBBS, MD (Biochemistry) · Reg. No. 21504

"Vitamin D deficiency is often silent. Early testing and correction can prevent long-term skeletal and metabolic complications."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeSerum
Sample Volume3 mL (2 mL min.)
ContainerSST (Serum Separator Tube)
Collection MethodVenipuncture

Sample Stability

Room temperature: 8 hours
Refrigerated: 72 hours
Frozen: 2 weeks
Sample Rejection Criteria:
  • Hemolyzed, lipemic, or icteric specimens
  • Improper labeling
  • Sample not transported within stability window

Understanding Your Results

The result report will clearly indicate your measured levels and classify them as deficient, insufficient, sufficient or toxic.
📊

Deficient – associated with bone disease, supplementation often recommended.

Level: <20 ng/mL

📊

Insufficient – suboptimal for bone health, consider Vitamin D supplementation.

Level: 20–29 ng/mL

📊

Sufficient – normal range for most individuals.

Level: 30–100 ng/mL

📊

Excessive/toxic – may cause hypercalcemia and requires medical evaluation.

Level: >100 ng/mL

⚠️ When to Consult a Doctor:

If your Vitamin D level is below 20 ng/mL or above 100 ng/mL, or if you have persistent bone pain, fatigue, or muscle weakness, consult an endocrinologist or general physician for further assessment.

Limitations

  • ⚠The test measures total 25(OH)D, not the active 1,25-dihydroxyvitamin D.
  • ⚠Seasonal and geographic variations affect normal reference ranges.
  • ⚠Different assay types may not be directly comparable.

Risks & Considerations

  • ●Mild pain or bruising at injection site
  • ●Dizziness or lightheadedness
  • ●Excessive bleeding (rare)
  • ●Infection (rare)

Interfering Factors

  • ●Hemolyzed specimens
  • ●Lipemic serum
  • ●Drugs like anticonvulsants, glucocorticoids, rifampin
  • ●Pregnancy alters vitamin D binding protein

Compare With Similar Tests

TestVitamin D Screen Test
ComparisonVitamin D Screen Test

Frequently Asked Questions

What is a Vitamin D Screen Test?
A Vitamin D Screen Test is a blood test that measures your 25-hydroxyvitamin D and calcium levels. It helps determine if you have a deficiency, insufficiency, or excess of Vitamin D, which is important for bone health and immunity.
Why is Vitamin D test done?
This test is done to evaluate symptoms like fatigue, bone pain, muscle weakness, and depression, or to assess fracture risk. It is also used to monitor response to Vitamin D supplementation and to work up conditions like malabsorption, renal disease, and disorders of calcium metabolism.
Do I need to fast for the Vitamin D Screen Test?
No, fasting is not required for the Vitamin D Screen Test. You can eat and drink normally before the test. However, remember to inform your doctor about any biotin supplements you take, as they can interfere with the assay.
What is the cost of the Vitamin D Screen Test at DNA Labs India?
The Vitamin D Screen Test at DNA Labs India costs INR 3000. This special discounted price includes free home sample collection across India, making it an affordable and convenient option.
How is the Vitamin D Screen Test performed?
A trained phlebotomist draws a venous blood sample from your arm into a serum separator tube. The sample is then processed using CLIA and spectrophotometry methods to measure 25-hydroxyvitamin D and calcium levels.
What is the normal range of Vitamin D 25 Hydroxy?
For most adults, a level of 30-100 ng/mL is considered sufficient. Levels below 20 ng/mL indicate deficiency, and 20-29 ng/mL indicate insufficiency. Levels above 100 ng/mL are considered potentially toxic and require medical evaluation.
What are the symptoms of Vitamin D deficiency?
Common symptoms include fatigue, bone pain, muscle weakness, depression, hair loss, and slow wound healing. Many people experience no symptoms initially, which is why testing is important for those at risk.
Who should get this test done?
People with symptoms suggestive of deficiency, those with risk factors like malabsorption, kidney or liver disease, obesity, older adults, individuals on anti-seizure or glucocorticoid medications, and those planning pregnancy or with a previous diagnosis of osteopenia or osteoporosis should consider this test.
When will I get my reports?
Reports are generally delivered within 24 hours after the sample reaches the laboratory. You will receive them through the online portal, email, or WhatsApp. Same-day reporting is available for samples received in the morning at our central lab.
Is home sample collection available for this test?
Yes, DNA Labs India provides free home sample collection for online bookings of the Vitamin D Screen Test. The service is available in Mumbai, Delhi, Bangalore, Hyderabad, Chennai, Kolkata, and more than 500 cities across India.
What if my Vitamin D levels are very low?
If your levels are below 20 ng/mL, your doctor may prescribe high-dose oral Vitamin D supplements, along with calcium if needed. Regular follow-up testing is recommended to monitor improvement. Always consult a physician before starting supplementation.
Are there any risks or complications of this test?
The test is generally safe. Minimal risks include mild pain, bruising, or bleeding at the injection site. Rarely, infection or excessive bleeding can occur. You can resume normal activities immediately after sample collection.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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