Complement-Dependent Cytotoxicity (CDC) Crossmatch Test
Short Name: CDC Crossmatch
Also known as: CDC Crossmatch, Complement-Dependent Cytotoxicity Crossmatch, T-cell Crossmatch, B-cell Crossmatch
Complement-Dependent Cytotoxicity (CDC) Crossmatch Test test available at DNA Labs India for ₹10,000. Uses Complement-Dependent Cytotoxicity (CDC) assay on Blood samples. Results in Results are typically available within 24-48 hours after the sample reaches the laboratory.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
The primary purpose of the CDC Crossmatch is to prevent hyperacute rejection, a rapid and irreversible immune response that can occur within minutes to hours after transplantation. By detecting donor-specific antibodies in the recipient's serum, the test helps transplant teams select compatible donor-recipient pairs, thereby improving graft survival and patient outcomes. It is also used to monitor sensitized patients, such as those who have had previous transplants, blood transfusions, or pregnancies, who may have developed antibodies against HLA antigens. Additionally, the test can be performed post-transplant to evaluate suspected antibody-mediated rejection.
- Test Code
- 6295
- CPT Code
- 86816
- ICD Code
- Z52.9
- Price
- ₹10,000
- Sample Type
- Blood
- Result Time
- Results are typically available within 24-48 hours after the sample reaches the laboratory.
- Fasting Required
- No
- Method
- Complement-Dependent Cytotoxicity (CDC) assay
Sample Collection
No special preparation is required. Inform your doctor about any medications you are taking, especially immunosuppressants or recent blood transfusions.
Method: Venipuncture
Laboratory Analysis
A blood sample will be drawn from a vein in your arm. The procedure is quick and causes minimal discomfort.
Report Delivery
You may resume normal activities immediately. There are no restrictions after the test.
Timeline: Results are typically available within 24-48 hours after the sample reaches the laboratory.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of the CDC Crossmatch is to prevent hyperacute rejection, a rapid and irreversible immune response that can occur within minutes to hours after transplantation. By detecting donor-specific antibodies in the recipient's serum, the test helps transplant teams select compatible donor-recipient pairs, thereby improving graft survival and patient outcomes. It is also used to monitor sensitized patients, such as those who have had previous transplants, blood transfusions, or pregnancies, who may have developed antibodies against HLA antigens. Additionally, the test can be performed post-transplant to evaluate suspected antibody-mediated rejection.
How to Prepare
- No fasting required
- Avoid blood transfusion for at least 72 hours before the test, if possible
- Inform the lab if you have had a recent transplant, transfusion, or pregnancy
- Ensure the sample is collected in a plain red-top tube and transported to the lab within 24 hours
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"The CDC crossmatch is a critical pre-transplant test that detects preformed donor-specific antibodies. A positive result indicates a high risk of hyperacute rejection, and the transplant is typically contraindicated. This test is essential for ensuring patient safety and improving graft survival."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed or lipemic sample
- Clotted sample
- Sample received after 24 hours of collection
- Improperly labeled sample
Understanding Your Results
Compatible; low risk of hyperacute rejection. Transplant can proceed.
High risk of hyperacute rejection; transplant is contraindicated.
May be due to anti-HLA class II antibodies or autoantibodies; requires further evaluation. Some centers may proceed with desensitization or consider the result as a relative contraindication.
If you are scheduled for a transplant and your CDC crossmatch result is positive, your transplant team will discuss the implications and possible alternatives, such as desensitization therapy or finding another donor. If you experience symptoms of rejection after a transplant (fever, pain, swelling, decreased organ function), contact your transplant coordinator immediately.
Limitations
- ⚠CDC crossmatch is less sensitive than flow cytometry or solid-phase assays; low-level antibodies may be missed
- ⚠Positive B-cell crossmatch may not always be clinically significant, especially if due to autoantibodies
- ⚠The test requires viable donor lymphocytes; if donor cells are not available, the test cannot be performed
- ⚠Results are qualitative and do not quantify antibody strength
- ⚠Interpretation requires correlation with clinical history and other tests
Risks & Considerations
- ●Minimal risk of bruising or bleeding at the puncture site
- ●Rare risk of infection or hematoma
Interfering Factors
- ●Recent blood transfusion (within 72 hours) may cause false positive due to alloantibodies
- ●Hemolyzed or lipemic serum samples may interfere with the assay
- ●Immunosuppressive drugs (e.g., rituximab) may affect B-cell crossmatch results
- ●Improper sample handling or storage may reduce cell viability
- ●High background cytotoxicity due to non-specific factors
Compare With Similar Tests
| Test | Complement-Dependent Cytotoxicity (CDC) Crossmatch | Flow Cytometry Crossmatch |
|---|---|---|
| Comparison | Complement-Dependent Cytotoxicity (CDC) Crossmatch | Flow cytometry is more sensitive than CDC and can detect lower levels of antibodies. It also provides quantitative data. However, CDC is more specific for complement-activating antibodies, which are most relevant for hyperacute rejection. Many centers use both tests for comprehensive evaluation. |
Frequently Asked Questions
What is the cost of CDC Crossmatch test in India?
What is the CDC Crossmatch test used for?
How is the CDC Crossmatch test performed?
What does a positive CDC Crossmatch mean?
Is fasting required for the CDC Crossmatch test?
What sample is needed for the CDC Crossmatch test?
How long does it take to get CDC Crossmatch results?
Can I get a home sample collection for CDC Crossmatch?
What is the difference between T-cell and B-cell crossmatch?
Are there any risks associated with the CDC Crossmatch test?
Can medications affect the CDC Crossmatch result?
What should I do if my CDC Crossmatch is positive?
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