Factor IX Functional Test
Also known as: Factor IX Activity Assay, Hemophilia B Diagnostic Test, Factor IX Clotting Test
Factor IX Functional Test test available at DNA Labs India for ₹3,000. Uses Photo-optical Clot Detection on Whole Blood samples. Results in Sample submitted on Wednesday or Saturday by 9 am; report available on Friday or Tuesday.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Gottipati Vemkateswarlu
Consultant Hematologist · Reg: AMC4946
Last reviewed: September 7, 2026
Overview
To diagnose hemophilia B, measure factor IX activity levels, assess disease severity, and guide treatment decisions such as replacement therapy.
- Test Code
- 537
- Price
- ₹3,000
- Sample Type
- Whole Blood
- Result Time
- Sample submitted on Wednesday or Saturday by 9 am; report available on Friday or Tuesday.
- Fasting Required
- Yes (8 hours)
- Method
- Photo-optical Clot Detection
Sample Collection
Overnight fasting is preferred. Discontinue Heparin for 1 day and Oral Anticoagulants for 7 days prior to sampling, with physician consent. Duly filled Coagulation Requisition Form (Form 15) is mandatory.
Method: Venipuncture
Laboratory Analysis
Blood sample collected via venipuncture into a sodium citrate tube, mixed by inversion to prevent clotting.
Report Delivery
Transport sample to lab within 4 hours. If delayed, prepare platelet-poor plasma (PPP) by centrifuging and freeze immediately. Do not thaw.
Timeline: Sample submitted on Wednesday or Saturday by 9 am; report available on Friday or Tuesday.
Patient Instructions
About This Test
Who Should Get This Test
To diagnose hemophilia B, measure factor IX activity levels, assess disease severity, and guide treatment decisions such as replacement therapy.
How to Prepare
- Collect 3 mL whole blood in 1 Blue Top (Sodium Citrate) tube
- Mix thoroughly by inversion
- Transport to lab within 4 hours of collection
- If transport delayed, prepare PPP within 1 hour and freeze immediately
Doctor's Notes
Reviewed by Dr Gottipati Vemkateswarlu — MBBS, MD (Haematology) · Reg. No. AMC4946
"This test is essential for diagnosing hemophilia B, determining severity, and guiding treatment to prevent bleeding complications."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Incorrect sample type or container
- Hemolyzed or clotted sample
- Insufficient sample volume
- Expired sample or improper storage
- Missing Coagulation Requisition Form
Understanding Your Results
If experiencing unexplained bleeding, easy bruising, or abnormal test results, consult a hematologist immediately for further evaluation and management.
Limitations
- ⚠May not detect mild or carrier states in some cases
- ⚠Results can be affected by pre-analytical errors
- ⚠Not a standalone test; may require additional coagulation tests
Risks & Considerations
- ●Minor bruising or hematoma at puncture site
- ●Rare risk of infection
- ●Dizziness or fainting (very rare)
Interfering Factors
- ●Heparin therapy (discontinue for 1 day prior)
- ●Oral anticoagulant therapy (discontinue for 7 days prior)
- ●Recent blood transfusions
- ●Improper sample handling or delayed processing
Compare With Similar Tests
| Test | Factor IX Functional Test | Factor VIII Functional Test | PT Test | APTT Test | Bleeding Time Test |
|---|---|---|---|---|---|
| Comparison | Factor IX Functional Test | Diagnoses hemophilia A vs. hemophilia B | Evaluates extrinsic pathway; Factor IX affects intrinsic pathway | Prolonged in hemophilia B; used for screening | Assesses platelet function; not specific for factor deficiencies |
Frequently Asked Questions
What is the Factor IX Functional Test?
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