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DNA Labs India

HLA Cadaveric Crossmatch Test

DNA Labs India | ISO 9001:2015 Certified

HLA Cadaveric Crossmatch Test

Short Name: HLA Crossmatch

Also known as: HLA Crossmatch, Cadaveric Crossmatch, Complement Dependent Cytotoxicity Crossmatch

HLA Cadaveric Crossmatch Test test available at DNA Labs India for ₹2,750. Uses Complement Dependent Cytotoxicity with DTT treatment on Whole blood and serum samples. Results in Sample collection to report: 10-12 days.. Free home collection in 300+ cities across India.

Nephrologist, Gastroenterologist🏠 Home Collection

✍️ Written By

Overview

To detect preformed donor-specific antibodies in recipient serum against cadaveric donor lymphocytes using complement-dependent cytotoxicity with DTT treatment, reducing the risk of hyperacute rejection.

Test Code
3479
Price
₹2,750
Sample Type
Whole blood and serum
Result Time
Sample collection to report: 10-12 days.
Fasting Required
No
Method
Complement Dependent Cytotoxicity with DTT treatment
Step 1

Sample Collection

Collect specimen preferably 48 hours after dialysis. Doctor's prescription on letterhead with stamp and photograph of recipient is mandatory.

Method: Venipuncture

Step 2

Laboratory Analysis

Standard venipuncture should be performed. Label samples accurately and maintain cold chain during transport.

Step 3

Report Delivery

Ship refrigerated. DO NOT FREEZE.

Timeline: Sample collection to report: 10-12 days.

Patient Instructions

1
Before the Test:Collect specimen preferably 48 hours after dialysis. Doctor's prescription on letterhead with stamp and photograph of recipient is mandatory.
2
During the Test:Standard venipuncture is performed. Label samples accurately and maintain cold chain.
3
After the Test:Ship refrigerated. DO NOT FREEZE.

About This Test

Who Should Get This Test

To detect preformed donor-specific antibodies in recipient serum against cadaveric donor lymphocytes using complement-dependent cytotoxicity with DTT treatment, reducing the risk of hyperacute rejection.

How to Prepare

  • Collect recipient sample preferably 48 hours after dialysis.
  • Use 6 mL whole blood in one Yellow Top ACD tube and 3 mL serum from one SST for recipient.
  • Use 12 mL whole blood in two Yellow Top ACD tubes for cadaveric donor.
  • Ship refrigerated; do not freeze.
  • Ensure doctor's prescription on letterhead with stamp and photograph of recipient is attached.

Doctor's Notes

"HLA crossmatch is essential before cadaveric organ transplantation to detect preformed donor-specific antibodies. A positive crossmatch is a strong contraindication to proceeding with that donor unless desensitisation protocols are planned. Always interpret with the transplant team."

Test Parameters & Specifications

Sample TypeWhole blood and serum
Sample VolumeRecipient: 6 mL whole blood + 3 mL serum; Cadaveric donor: 12 mL whole blood
ContainerYellow Top (ACD) tubes and SST
Collection MethodVenipuncture

Sample Stability

Room temperature: NA
Refrigerator: 48 hours
Frozen: NA
Sample Rejection Criteria:
  • Frozen samples
  • Hemolyzed samples
  • Insufficient sample volume
  • Unlabelled or mislabelled samples
  • Missing mandatory prescription or recipient photograph

Understanding Your Results

A negative crossmatch suggests no preformed donor-specific antibodies were detected and the donor may be considered compatible from a CDC crossmatch perspective. A positive crossmatch indicates antibodies that may cause hyperacute or accelerated rejection.
Negative T-cell and B-cell crossmatch: Lower risk of CDC-defined incompatibility.
Positive T-cell crossmatch: High risk of antibody-mediated rejection; usually contraindicated.
Positive B-cell crossmatch only: May be due to autoantibodies or non-HLA antibodies; correlate clinically.
DTT treatment helps remove IgM; persistent reactivity after DTT suggests IgG antibodies.
⚠️ When to Consult a Doctor:

If the crossmatch is positive or if the transplant team needs further risk assessment; all transplant decisions must be made by the treating nephrologist or gastroenterologist.

Limitations

  • ⚠Does not detect non-HLA antibodies or low-level antibodies below CDC sensitivity
  • ⚠Requires viable donor lymphocytes; cadaveric donor cells must be properly collected
  • ⚠DTT treatment may not eliminate IgM unless protocol is validated
  • ⚠Negative crossmatch does not guarantee no rejection
  • ⚠Must be interpreted with HLA typing and clinical risk assessment

Risks & Considerations

  • ●Standard venipuncture risks include mild pain, bruising, or hematoma.
  • ●No additional risk from the test itself.

Interfering Factors

  • ●Hemolysis, lipemia, or bacterial contamination of samples
  • ●Insufficient sample volume
  • ●Freezing of specimens may reduce lymphocyte viability
  • ●Recent dialysis timing can affect results; collect preferably 48 hours after dialysis
  • ●Improper labelling or missing mandatory prescription and recipient photograph

Compare With Similar Tests

TestHLA Cadaveric Crossmatch Test
ComparisonHLA Cadaveric Crossmatch Test

Frequently Asked Questions

What is HLA cadaveric crossmatch test?
HLA cadaveric crossmatch is a laboratory test that checks whether a deceased donor's organ is compatible with the recipient's immune system before transplant surgery.
Why is this test done before organ transplant?
It is done to detect preformed donor-specific antibodies in the recipient that could attack the transplanted organ and cause hyperacute or accelerated rejection.
What type of sample is required?
The recipient sample includes 6 mL whole blood in an ACD tube and 3 mL serum from an SST. The cadaveric donor sample includes 12 mL whole blood in two ACD tubes.
How is the test performed?
The test mixes recipient serum with donor lymphocytes using complement-dependent cytotoxicity with DTT treatment to detect cell death caused by preformed antibodies.
What does a positive crossmatch mean?
A positive crossmatch means the recipient has antibodies against donor HLA antigens, which increases the risk of organ rejection. It is usually a contraindication to transplant with that donor.
What does a negative crossmatch mean?
A negative crossmatch means no significant preformed donor-specific antibodies were detected by this method, suggesting lower risk of CDC-defined incompatibility.
What is DTT treatment?
DTT treatment is used in the crossmatch procedure to remove or reduce IgM antibodies, helping to distinguish clinically significant IgG antibodies from less relevant IgM reactivity.
How long does it take to get reports?
Reports are usually available within 10-12 days after sample collection and laboratory processing.
Do I need to fast before this test?
No fasting is required. However, specimen collection should preferably be done 48 hours after dialysis as per the pre-test instruction.
Is home sample collection available?
Yes, DNA Labs India offers free home sample collection for online bookings for the HLA Cadaveric Crossmatch Test across India.
What is the cost of HLA cadaveric crossmatch test?
The cost of the HLA Cadaveric Crossmatch Test at DNA Labs India is Rs 2750.0.
Can this test be done for living donors too?
This protocol is specifically for cadaveric donors. Living donor crossmatch may require separate collection requirements and should be discussed with the transplant team.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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