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HLA Single Antigen Bead Assay for Class I & II IgG Antibodies DSA-SAB Class I & II Test

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HLA Single Antigen Bead Assay for Class I & II IgG Antibodies DSA-SAB Class I & II Test

Short Name: HLA SAB Class I & II Test

Also known as: DSA-SAB Test, HLA Antibody Test, Donor-Specific Antibody Assay, Luminex HLA Antibody Test

HLA Single Antigen Bead Assay for Class I & II IgG Antibodies DSA-SAB Class I & II Test test available at DNA Labs India for ₹50,000. Uses Luminex X-Map technology on Serum samples. Results in 10-12 days. Free home collection in 300+ cities across India.

ImmunoassayAdults🏠 Home Collection

✍️ Written By

Overview

The primary purpose of the HLA Single Antigen Bead Assay is to detect the presence of IgG antibodies specific to HLA antigens, which indicate an immune response that could lead to transplant rejection. It is used to evaluate sensitization risk before transplantation and monitor for donor-specific antibodies post-transplant, enabling early intervention to prevent graft failure.

Test Code
823
Price
₹50,000
Sample Type
Serum
Result Time
10-12 days
Fasting Required
No
Method
Luminex X-Map technology
Step 1

Sample Collection

Provide a brief clinical history, including transplant details and current medications. No specific fasting is required, but inform about any recent transfusions.

Method: Venipuncture

Step 2

Laboratory Analysis

A standard blood draw from a vein in the arm, typically taking 5-10 minutes.

Step 3

Report Delivery

Apply pressure to the site to prevent bleeding. Resume normal activities unless otherwise advised.

Timeline: 10-12 days

Patient Instructions

1
Before the Test:Ensure clinical history is provided. No special preparation is needed, but avoid strenuous activity before the blood draw.
2
During the Test:A blood sample is drawn from a vein. The process is similar to routine blood tests.
3
After the Test:Apply pressure to the puncture site. Results are available online after 10-12 days.

About This Test

Who Should Get This Test

The primary purpose of the HLA Single Antigen Bead Assay is to detect the presence of IgG antibodies specific to HLA antigens, which indicate an immune response that could lead to transplant rejection. It is used to evaluate sensitization risk before transplantation and monitor for donor-specific antibodies post-transplant, enabling early intervention to prevent graft failure.

How to Prepare

  • Collect 2 mL serum in an SST tube
  • Ship sample refrigerated (2-8°C) or frozen (-20°C)
  • Label with patient details and clinical history

Doctor's Notes

"This test is crucial for transplant patients to detect donor-specific antibodies early, enabling proactive management to prevent rejection and ensure long-term graft survival."

Test Parameters & Specifications

Sample TypeSerum
Sample Volume2 mL (minimum 1 mL)
ContainerSST (Serum Separator Tube)
Collection MethodVenipuncture

Sample Stability

Room Temperature: Up to 6 hours
Refrigerator (2-8°C): Up to 72 hours
Frozen (-20°C): Up to 1 week
Sample Rejection Criteria:
  • Insufficient sample volume (less than 1 mL)
  • Hemolyzed or contaminated sample
  • Improper labeling or missing clinical history

Understanding Your Results

Results indicate the presence and specificity of HLA antibodies. Positive results suggest sensitization, which may require immunosuppressive adjustment or further testing.
📊

Negative for all HLA antigens

Low risk of antibody-mediated rejection. Continue routine monitoring.

📊

Positive for donor-specific antibodies

High risk of rejection. Consider therapy adjustment and closer follow-up.

📊

Positive for non-donor-specific antibodies

May indicate general sensitization. Monitor and assess clinical significance.

📊

High MFI values

Strong antibody response, associated with higher rejection risk.

⚠️ When to Consult a Doctor:

If results show positive HLA antibodies, especially donor-specific, or if there are symptoms of rejection such as fever, fatigue, decreased organ function, or pain at the transplant site.

Limitations

  • ⚠May not detect all antibody subtypes (e.g., IgM or IgA)
  • ⚠Results require correlation with clinical history and other tests
  • ⚠False positives can occur due to cross-reactivity
  • ⚠Does not provide information on antibody function or affinity

Risks & Considerations

  • ●Minor bruising or pain at the blood draw site
  • ●Rare risk of infection or hematoma
  • ●No significant systemic risks

Interfering Factors

  • ●Hemolyzed or lipemic serum samples
  • ●Presence of aggregated proteins or immune complexes
  • ●Recent transfusions or immunizations
  • ●Use of certain immunosuppressive medications

Frequently Asked Questions

What is the HLA Single Antigen Bead Assay?
It is a test that detects IgG antibodies against HLA antigens in the blood, used to assess transplant rejection risk.
Why is this test important for transplant patients?
It helps identify donor-specific antibodies that can cause organ rejection, allowing for timely medical intervention.
What sample is required for this test?
A blood sample of 2 mL serum collected in an SST tube.
Is fasting required before the test?
No, fasting is not required, but provide your clinical history.
How much does the test cost?
The test costs INR 50,000 in India, with home collection available.
How long does it take to get results?
Results are typically available in 10-12 days.
What does a positive result mean?
It indicates the presence of HLA antibodies, which may increase rejection risk; consult your doctor.
Are there any risks associated with the test?
The test involves a standard blood draw with minimal risks like bruising.
Can this test be done at home?
Yes, free home sample collection is available across India for online bookings.
What HLA antigens does the test detect?
It detects antibodies against HLA-A, B, C, DR, DQA1, DQB1, DPA1, and DPB1 antigens.
How should the sample be stored and transported?
The serum sample should be shipped refrigerated or frozen as per instructions.
What if my results are abnormal?
Consult your nephrologist or transplant specialist for further evaluation and management options.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

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