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HLA T & B Cell Flow Cytometry Crossmatch Test

DNA Labs India | ISO 9001:2015 Certified

HLA T & B Cell Flow Cytometry Crossmatch Test

Short Name: HLA Crossmatch Test

Also known as: Flow Cytometry Crossmatch, HLA Crossmatch Test, T&B Cell Crossmatch

HLA T & B Cell Flow Cytometry Crossmatch Test test available at DNA Labs India for ₹10,000. Uses Flow Cytometry on Serum and Whole Blood samples. Results in 10-12 days. Free home collection in 300+ cities across India.

Immunological Test🏠 Home Collection

✍️ Written By

Overview

To determine the compatibility of donor organ with the recipient by detecting anti-donor antibodies that could cause organ rejection.

Test Code
825
Price
₹10,000
Sample Type
Serum and Whole Blood
Result Time
10-12 days
Fasting Required
No
Method
Flow Cytometry
Step 1

Sample Collection

Ensure doctor's prescription on letterhead with stamp, photographs of recipient and donor, and Solid Organ Transplant Consent form (Form 33) are prepared. Collect specimen preferably 48 hours after dialysis if applicable.

Method: Venipuncture

Step 2

Laboratory Analysis

Blood samples are collected from recipient and donor using standard venipuncture techniques into specified containers (SST and sodium heparin tubes).

Step 3

Report Delivery

Label samples clearly and ship immediately at 18-22°C. Do not freeze. Ensure proper documentation accompanies the specimens.

Timeline: 10-12 days

Patient Instructions

1
Before the Test:Follow pre-collection instructions, including timing relative to dialysis and document preparation.
2
During the Test:Blood samples are processed in the laboratory using flow cytometry to analyze antibody binding to donor cells.
3
After the Test:Results are reviewed and reported by laboratory specialists after thorough analysis.

About This Test

Who Should Get This Test

To determine the compatibility of donor organ with the recipient by detecting anti-donor antibodies that could cause organ rejection.

How to Prepare

  • Collect recipient serum (2 mL) and whole blood (4 mL) as specified.
  • Collect donor whole blood (3 mL) in sodium heparin tubes.
  • Ship samples immediately at room temperature (18-22°C).
  • Include doctor's prescription with stamp and photographs of recipient and donor.
  • Submit completed Solid Organ Transplant Consent form (Form 33).

Doctor's Notes

"This test is critical for evaluating donor-recipient compatibility in organ transplants, helping to minimize the risk of rejection and improve outcomes."

Test Parameters & Specifications

Sample TypeSerum and Whole Blood
Sample VolumeRecipient: 2 mL serum + 4 mL whole blood; Donor: 3 mL whole blood
ContainerSST tube and Green Top (Sodium Heparin) tubes
Collection MethodVenipuncture

Sample Stability

Room Temperature
Refrigerator
Frozen
Sample Rejection Criteria:
  • Insufficient sample volume
  • Incorrect sample container used
  • Missing or incomplete documentation
  • Hemolyzed or clotted samples
  • Samples not shipped at specified temperature

Understanding Your Results

The HLA T & B Cell Flow Cytometry Crossmatch Test results indicate the presence or absence of anti-donor antibodies, which are critical for assessing transplant compatibility.
📊

Negative

No detectable anti-donor antibodies against T or B cells; suggests compatibility for transplantation with lower rejection risk.

📊

Positive

Anti-donor antibodies detected; indicates a higher risk of organ rejection, requiring further evaluation and potential desensitization strategies.

⚠️ When to Consult a Doctor:

If you experience symptoms of organ rejection post-transplant, such as fever, chills, shortness of breath, pain over the transplant site, swelling, decreased urine output, increased blood pressure, or weight gain, contact your doctor or transplant team immediately.

Limitations

  • ⚠Cannot detect all antibody subclasses
  • ⚠Requires specialized flow cytometry equipment
  • ⚠May have false positives/negatives in certain conditions
  • ⚠Time-consuming process with longer turnaround

Risks & Considerations

  • ●Minor bruising or pain at the blood draw site
  • ●Rare risk of infection
  • ●Potential for fainting or dizziness

Interfering Factors

  • ●Recent blood transfusions or plasma exchange
  • ●Presence of infections or autoimmune disorders
  • ●Use of immunosuppressive medications
  • ●Sample hemolysis or improper handling

Frequently Asked Questions

What is the HLA T & B Cell Flow Cytometry Crossmatch Test?
It is a laboratory test that uses flow cytometry to detect antibodies in a recipient's blood that may react against a donor's HLA antigens, assessing compatibility for organ transplantation.
Why is this test necessary for organ transplantation?
It helps identify potential antibody-mediated rejection risks, ensuring donor-recipient compatibility to improve transplant success rates.
How is the test performed?
Blood samples from the recipient and donor are mixed and analyzed using flow cytometry to detect antibodies that bind to donor T and B cells.
What is the cost of the HLA T & B Cell Flow Cytometry Crossmatch Test in India?
The test costs INR 10000 at DNA Labs India, with free home collection available across many cities.
Is home sample collection available for this test?
Yes, DNA Labs India offers free home sample collection for online bookings in numerous cities across India.
How long does it take to get the results?
Results are typically available within 10-12 days after sample collection.
What are the symptoms of organ rejection?
Symptoms include fever, chills, shortness of breath, pain over the transplanted organ, swelling, decreased urine output, increased blood pressure, and weight gain.
Can the test be done after dialysis?
Yes, it is recommended to collect the specimen preferably 48 hours after dialysis for accurate results.
What documents are required for the test?
Doctor's prescription on letterhead with stamp, photographs of recipient and donor, and Solid Organ Transplant Consent form (Form 33) are mandatory.
Is fasting required before the test?
No, fasting is not required, but timing relative to dialysis should be considered.
What happens if the test is positive?
A positive result indicates anti-donor antibodies, which may require further evaluation, desensitization therapy, or reconsideration of the donor.
How accurate is the HLA T & B Cell Flow Cytometry Crossmatch Test?
The test is highly sensitive and specific, capable of detecting antibodies at low levels, making it a reliable tool for transplant compatibility assessment.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

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✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

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